A synthetic mechano-growth factor E peptide promotes rat tenocyte migration by lessening cell stiffness and increasing F-actin formation via the FAK-ERK1/2 signaling pathway.

Zhang B, Luo Q, Mao X, Xu B, Yang L, Ju Y, Song G

Experimental cell research13 Jan 2014Cited by 27 (Europe PMC)

PMID24434354DOI10.1016/j.yexcr.2014.01.005PepBankPUB-24434354

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study9

6 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Activity measurement
In a scratch wound assay with rat tenocytes, MGF-C25E promoted cell migration.
In plain wordsIn rat tendon cells in a lab dish, a synthetic MGF peptide made the cells move into a scratch faster.
Peptide
MGF / PEG-MGF
Outcome
IncreaseTenocyte migration
Study
ratn: not reported in source
rat tenocytes
Outcome group
Tendon and ligament healing · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
We used a scratch wound assay in this study to demonstrate that MGF-C25E (a synthetic mechano-growth factor E peptide) promotes the migration of rat tenocytes
Report a problemFND-2443435401
02LabIn vitro (cells)Mechanism
In rat tenocytes, MGF-C25E was accompanied by higher expression of focal adhesion kinase (FAK).
In plain wordsIn rat tendon cells in a lab dish, a synthetic MGF peptide was linked to more of the signal protein FAK.
Peptide
MGF / PEG-MGF
Outcome
IncreaseFAK expression
Study
ratn: not reported in source
rat tenocytes
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
an elevation in the expression of the following signaling molecules: focal adhesion kinase (FAK)
Report a problemFND-2443435402
03LabIn vitro (cells)Mechanism
In rat tenocytes, MGF-C25E was accompanied by higher expression of ERK1/2.
In plain wordsIn rat tendon cells in a lab dish, a synthetic MGF peptide was linked to more of the signal protein ERK1/2.
Peptide
MGF / PEG-MGF
Outcome
IncreaseERK1/2 expression
Study
ratn: not reported in source
rat tenocytes
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
an elevation in the expression of the following signaling molecules: focal adhesion kinase (FAK) and extracellular signal regulated kinase1/2 (ERK1/2)
Report a problemFND-2443435403
04LabIn vitro (cells)Mechanism
In rat tenocytes, MGF-C25E treatment decreased cell stiffness measured as Young's modulus.
In plain wordsIn rat tendon cells in a lab dish, a synthetic MGF peptide made the cells softer.
Peptide
MGF / PEG-MGF
Outcome
DecreaseTenocyte Young's modulus (cell stiffness)
Study
ratn: not reported in source
rat tenocytes
Outcome group
Tendon and ligament healing · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
modulus of tenocytes was decreased through treatment of MGF-C25E, and an obvious formation of pseudopodia and F-actin was observed in MGF-C25E-treated tenocytes.
Report a problemFND-2443435405
05LabIn vitro (cells)Mechanism
In rat tenocytes, MGF-C25E treatment increased the formation of pseudopodia.
In plain wordsIn rat tendon cells in a lab dish, a synthetic MGF peptide caused more cell protrusions to form.
Peptide
MGF / PEG-MGF
Outcome
IncreasePseudopodia formation
Study
ratn: not reported in source
rat tenocytes
Outcome group
Tendon and ligament healing · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
an obvious formation of pseudopodia and F-actin was observed in MGF-C25E-treated tenocytes.
Report a problemFND-2443435406
06LabIn vitro (cells)Mechanism
In rat tenocytes, MGF-C25E treatment increased the formation of F-actin.
In plain wordsIn rat tendon cells in a lab dish, a synthetic MGF peptide caused more F-actin to form.
Peptide
MGF / PEG-MGF
Outcome
IncreaseF-actin formation
Study
ratn: not reported in source
rat tenocytes
Outcome group
Tendon and ligament healing · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
an obvious formation of pseudopodia and F-actin was observed in MGF-C25E-treated tenocytes.
Report a problemFND-2443435407

Studies that did not test the peptide alone3

In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking MGF / PEG-MGF.

07LabNot a test of MGF / PEG-MGF aloneIn vitro (cells)Mechanism
In rat tenocytes, FAK and ERK1/2 pathway inhibitors blocked the migration induced by MGF-C25E.
In plain wordsIn rat tendon cells in a lab dish, blocking FAK or ERK1/2 stopped the extra movement caused by a synthetic MGF peptide.
Peptide
MGF / PEG-MGF
Outcome
DecreaseMigration after FAK or ERK1/2 inhibition
Study
ratn: not reported in source
rat tenocytes
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
Inhibitors of the FAK and ERK1/2 pathways inhibited the MGF-C25E-induced tenocyte migration, indicating that MGF-C25E promotes tenocyte migration through the FAK-ERK1/2 signaling pathway.
In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.
Report a problemFND-2443435404
08LabNot a test of MGF / PEG-MGF aloneIn vitro (cells)Mechanism
In rat tenocytes, inhibiting FAK or ERK1/2 restored the decrease in Young's modulus caused by MGF-C25E.
In plain wordsIn rat tendon cells in a lab dish, blocking FAK or ERK1/2 undid the softening caused by a synthetic MGF peptide.
Peptide
MGF / PEG-MGF
Outcome
IncreaseYoung's modulus after FAK or ERK1/2 inhibition
Study
ratn: not reported in source
rat tenocytes
Outcome group
Tendon and ligament healing · Musculoskeletal
Source sentences
Summary
The inhibition of the FAK or ERK1/2 signals restored the decrease in Young's modulus and inhibited the formation of pseudopodia and F-actin.
In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.
Report a problemFND-2443435408
09LabNot a test of MGF / PEG-MGF aloneIn vitro (cells)Mechanism
In rat tenocytes, inhibiting FAK or ERK1/2 reduced the formation of pseudopodia and F-actin seen with MGF-C25E.
In plain wordsIn rat tendon cells in a lab dish, blocking FAK or ERK1/2 reduced the protrusions and F-actin caused by a synthetic MGF peptide.
Peptide
MGF / PEG-MGF
Outcome
DecreasePseudopodia and F-actin after FAK or ERK1/2 inhibition
Study
ratn: not reported in source
rat tenocytes
Outcome group
Tendon and ligament healing · Musculoskeletal
Source sentences
Summary
The inhibition of the FAK or ERK1/2 signals restored the decrease in Young's modulus and inhibited the formation of pseudopodia and F-actin.
In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.
Report a problemFND-2443435409

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

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