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Study · In vitro (cells) · mouse · 2014

6 results

Mechano-growth factor peptide, the COOH terminus of unprocessed insulin-like growth factor 1, has no apparent effect on myoblasts or primary muscle stem cells.

Fornaro M, Hinken AC, Needle S, Hu E, Trendelenburg AU, Mayer A, Rosenstiel A, Chang C, Meier V, Billin AN, Becherer JD, Brace AD, Evans WJ, Glass DJ, Russell AJ

American journal of physiology. Endocrinology and metabolism19 Nov 2013Cited by 38 (Europe PMC)

PMID24253050 (opens in a new tab)DOI10.1152/ajpendo.00408.2013 (opens in a new tab)PepBankPUB-24253050
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In C2C12 cells, MGF peptide at concentrations up to 500 ng/ml failed to increase proliferation.

    In plain wordsIn cells, the MGF peptide did not make C2C12 muscle cells multiply, even at the highest amount tested.

    Peptide
    MGF / PEG-MGF
    Outcome
    No significant changeC2C12 cell proliferation
    Study

    n: not reported in source

    C2C12 cells
    Outcome group
    Cell proliferation and differentiation · Musculoskeletal
    Studied dose
    up to 500 ng/mlAs given in this study.

    Source sentences

    Summary
    Concentrations of peptide up to 500 ng/ml failed to increase the proliferation of C2C12 cells or primary human skeletal muscle myoblasts.
    1 context sentence
    Summary · context
    Thus, at our two pharmaceutical companies, we attempted to reproduce the claimed effect of MGF peptides on human and mouse muscle myoblast proliferation and differentiation in vitro.
  2. 02LabIn vitro (cells)Efficacy outcome

    In primary human skeletal muscle myoblasts, MGF peptide at concentrations up to 500 ng/ml failed to increase proliferation.

    In plain wordsIn cells, the MGF peptide did not make human muscle cells multiply, even at the highest amount tested.

    Peptide
    MGF / PEG-MGF
    Outcome
    No significant changePrimary human skeletal muscle myoblast proliferation
    Study

    humann: not reported in source

    primary human skeletal muscle myoblasts
    Outcome group
    Cell proliferation and differentiation · Musculoskeletal
    Studied dose
    up to 500 ng/mlAs given in this study (human).

    Source sentences

    Summary
    Concentrations of peptide up to 500 ng/ml failed to increase the proliferation of C2C12 cells or primary human skeletal muscle myoblasts.
    1 context sentence
    Summary · context
    Thus, at our two pharmaceutical companies, we attempted to reproduce the claimed effect of MGF peptides on human and mouse muscle myoblast proliferation and differentiation in vitro.
  3. 03LabIn vitro (cells)Efficacy outcome

    In muscle myoblasts, MGF failed to inhibit differentiation of myoblasts into myotubes.

    In plain wordsIn cells, MGF did not stop muscle cells from maturing into muscle fibres.

    Peptide
    MGF / PEG-MGF
    Outcome
    No significant changeMyoblast differentiation into myotubes
    Study

    n: not reported in source

    muscle myoblasts
    Outcome group
    Muscle injury and repair · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    MGF also failed to inhibit the differentiation of myoblasts into myotubes.
    1 context sentence
    Summary · context
    Thus, at our two pharmaceutical companies, we attempted to reproduce the claimed effect of MGF peptides on human and mouse muscle myoblast proliferation and differentiation in vitro.
  4. 04LabIn vitro (cells)Efficacy outcome

    In primary mouse skeletal muscle stem cells, MGF failed to show a significant effect on proliferation.

    In plain wordsIn mouse cells, MGF did not have a clear effect on how fast muscle stem cells multiplied.

    Peptide
    MGF / PEG-MGF
    Outcome
    No significant changePrimary mouse skeletal muscle stem cell proliferation
    Study

    mousen: not reported in source

    primary mouse skeletal muscle stem cells
    Outcome group
    Cell proliferation and differentiation · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    To address whether the response to MGF was lost in these tissue culture lines, we measured proliferation and differentiation of primary mouse skeletal muscle stem cells exposed to MGF. This, too, failed to demonstrate a significant effect.
  5. 05LabIn vitro (cells)Efficacy outcome

    In primary mouse skeletal muscle stem cells, MGF failed to show a significant effect on differentiation.

    In plain wordsIn mouse cells, MGF did not have a clear effect on how muscle stem cells matured.

    Peptide
    MGF / PEG-MGF
    Outcome
    No significant changePrimary mouse skeletal muscle stem cell differentiation
    Study

    mousen: not reported in source

    primary mouse skeletal muscle stem cells
    Outcome group
    Muscle injury and repair · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    To address whether the response to MGF was lost in these tissue culture lines, we measured proliferation and differentiation of primary mouse skeletal muscle stem cells exposed to MGF. This, too, failed to demonstrate a significant effect.
  6. 06LabIn vitro (cells)Mechanism

    In cardiac myocytes, native or stabilized MGF peptide showed no activating response in p-ERK.

    In plain wordsIn cells, neither natural nor stabilized MGF peptide switched on the ERK signal in heart muscle cells.

    Peptide
    MGF / PEG-MGF
    Outcome
    No significant changep-ERK activation in cardiac myocytes
    Study

    n: not reported in source

    cardiac myocytes
    Outcome group
    Receptor activity and mechanism · Cardiovascular
    Studied dose
    Dose not stated

    Source sentences

    Summary
    there were no demonstrated activating responses from the native or a stabilized MGF peptide.
    1 context sentence
    Summary · context
    Finally, we tested whether MGF could alter a separate documented in vitro effect of the peptide, activation of p-ERK, but not p-Akt, in cardiac myocytes.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

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Terms as the source lists them.

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