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Study · Not a test of the peptide alone · 2014

4 results

Analytics of the therapeutic peptide aviptadil by sheathless CE-MS and comparison with nanoRP-HPLC-MS.

Gross PC, Burkart SC, Müller R

Journal of pharmaceutical and biomedical analysis5 Oct 2013Cited by 6 (Europe PMC)

PMID24176753 (opens in a new tab)DOI10.1016/j.jpba.2013.09.024 (opens in a new tab)PepBankPUB-24176753
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone4

In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).

  1. 01LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cell-free)Other

    In an analytical comparison, CE-MS detected 15 impurities in aviptadil, and nanoRP-HPLC-MS detected two.

    In plain wordsIn lab tests, one analysis method found 15 impurities in aviptadil and another found only two.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableImpurities detected in aviptadil by CE-MS and nanoRP-HPLC-MS
    Study

    n: not reported in source

    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    Impurities detected
    15 impurities
    CE-MS

    Source sentences

    Summary
    The basic therapeutic peptide aviptadil served as a model substance to study the impurity profiles revealing 15 detectable impurities using CE-MS, two were detected by an appropriate nanoRP-HPLC-MS method.
    1 context sentence
    Summary · context
    As usage of an orthogonal technique is highly advisable for quality assurance, capillary electrophoresis (CE) employing a coated capillary coupled via a sheathless interface to a mass spectrometer was applied in parallel.

    In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).

  2. 02LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cell-free)Other

    None of the aviptadil impurities detected by CE-MS were observed by LC, and the reverse also held.

    In plain wordsIn lab tests, the two methods found different impurities in aviptadil, with no overlap.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableOverlap of impurities between CE-MS and LC
    Study

    n: not reported in source

    Outcome group
    Analytical methods and detection · Pharmacology

    Source sentences

    Summary
    None of the impurities detected by CE were observed in LC and vice versa.
    1 context sentence
    Summary · context
    As usage of an orthogonal technique is highly advisable for quality assurance, capillary electrophoresis (CE) employing a coated capillary coupled via a sheathless interface to a mass spectrometer was applied in parallel.

    In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).

  3. 03LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cell-free)Other

    The limit of detection for aviptadil was about 1 fmol in CE-MS, 2500 times lower than the 3 pmol in nanoRP-HPLC-MS.

    In plain wordsIn lab tests, one method detected much smaller amounts of aviptadil than the other.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableLimit of detection of aviptadil
    Study

    n: not reported in source

    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    LOD
    ≈ 1 fmol
    CE-MS
    LOD
    3 pmol
    nanoRP-HPLC-MS

    Source sentences

    Summary
    The LOD in CE-MS was determined in the base peak electropherogram at ∼1fmol, a value 2500 times smaller than the LOD found in nanoRP-HPLC-MS (3pmol).
    1 context sentence
    Summary · context
    As usage of an orthogonal technique is highly advisable for quality assurance, capillary electrophoresis (CE) employing a coated capillary coupled via a sheathless interface to a mass spectrometer was applied in parallel.

    In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).

  4. 04LabNot a test of Vasoactive intestinal peptide (VIP, aviptadil) aloneIn vitro (cell-free)Other

    In nanoRP-HPLC-MS, only 0.2% of the extrapolated CE-MS signal was observed for a 25 ng aviptadil load.

    In plain wordsIn lab tests, the HPLC method saw only a tiny part of the aviptadil signal that the other method saw.

    Peptide
    Vasoactive intestinal peptide (VIP, aviptadil)
    Outcome
    Not applicableAviptadil signal recovery in nanoRP-HPLC-MS
    Study

    n: not reported in source

    Outcome group
    Analytical methods and detection · Pharmacology

    Measurements

    Signal relative to CE-MS
    0.2%
    25 ng aviptadil, nanoRP-HPLC-MS

    Source sentences

    Summary
    In nanoRP-HPLC-MS only 0.2% of the extrapolated CE-MS signal for a 25ng aviptadil load was observed.
    1 context sentence
    Summary · context
    As usage of an orthogonal technique is highly advisable for quality assurance, capillary electrophoresis (CE) employing a coated capillary coupled via a sheathless interface to a mass spectrometer was applied in parallel.

    In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    12 Oct 2026, 12:58 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 12:40 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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