The effect of human chorionic gonadotropin-based hormonal therapy on intratesticular testosterone levels and spermatogonial DNA synthesis in men with non-obstructive azoospermia.

Shinjo E, Shiraishi K, Matsuyama H

Andrology2 Oct 2013Cited by 54 (Europe PMC)

PMID24123916DOI10.1111/j.2047-2927.2013.00141.xPepBankPUB-24123916

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone8

In this study, hCG (human chorionic gonadotropin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking hCG (human chorionic gonadotropin).

01HumanNot a test of hCG (human chorionic gonadotropin) aloneNon-randomized trialEfficacy outcome
In men with non-obstructive azoospermia, hCG-based hormonal therapy preceded successful sperm retrieval in three men (15%).
In plain wordsIn men with non-obstructive azoospermia, sperm retrieval succeeded in 15% after hCG-based hormonal therapy.
Peptide
hCG (human chorionic gonadotropin)
Outcome
Not applicableSperm retrieval
Study
n = 20
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
Men with successful sperm retrieval
3 men
Second micro-TESE after hCG-based hormonal therapy
Successful sperm retrieval
15%
Second micro-TESE after hCG-based hormonal therapy
Source sentences
Summary
During the second micro-TESE, spermatozoa were successfully retrieved from three (15%) of the men who had been treated with hormonal therapy.
1 context sentence(design, population, supporting numbers)
Summary · context
Twenty patients who failed sperm retrieval procedures using microdissection testicular sperm extraction (micro-TESE) were enrolled in hCG-based hormonal therapy prior to a second micro-TESE.
In this study, hCG (human chorionic gonadotropin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking hCG (human chorionic gonadotropin).
Report a problemFND-2412391601
02HumanNot a test of hCG (human chorionic gonadotropin) aloneNon-randomized trialEfficacy outcome
In men with non-obstructive azoospermia, PCNA-LI increased significantly after hCG-based hormonal therapy.
In plain wordsIn men with non-obstructive azoospermia, hCG-based hormonal therapy increased a marker of sperm-cell DNA synthesis.
Peptide
hCG (human chorionic gonadotropin)
Outcome
IncreasePCNA labelling index
Study
n = 20
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
PCNA-positive cells were predominantly in the spermatogonia and primary spermatocytes, and PCNA-LI was significantly increased after the hormonal therapy.
1 context sentence(design, population, supporting numbers)
Summary · context
Twenty patients who failed sperm retrieval procedures using microdissection testicular sperm extraction (micro-TESE) were enrolled in hCG-based hormonal therapy prior to a second micro-TESE.
In this study, hCG (human chorionic gonadotropin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking hCG (human chorionic gonadotropin).
Report a problemFND-2412391602
03HumanNot a test of hCG (human chorionic gonadotropin) aloneNon-randomized trialEfficacy outcome
In men with non-obstructive azoospermia, hCG-based hormonal therapy increased ITT from 273.6 ± 134.4 to 1348.1 ± 505.4 ng/mL.
In plain wordsIn men with non-obstructive azoospermia, hCG-based hormonal therapy increased testosterone inside the testes.
Peptide
hCG (human chorionic gonadotropin)
Outcome
IncreaseIntratesticular testosterone
Study
n = 20
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
Intratesticular testosterone
273.6 ng/mL
Before hCG-based hormonal therapy
Reported plus/minus uncertainty of intratesticular testosterone, type unspecified
134.4 ng/mL
Before hCG-based hormonal therapy
Intratesticular testosterone
1348.1 ng/mL
After hCG-based hormonal therapy
Reported plus/minus uncertainty of intratesticular testosterone, type unspecified
505.4 ng/mL
After hCG-based hormonal therapy
p value for intratesticular testosterone before versus after hormonal therapy
< 0.0001 p value
Before versus after hCG-based hormonal therapy
Source sentences
Summary
A significant increase in the ITT levels before and after the hormonal therapy (p < 0.0001, 273.6 ± 134.4 and 1348.1 ± 505.4 ng/mL respectively).
1 context sentence(design, population, supporting numbers)
Summary · context
Twenty patients who failed sperm retrieval procedures using microdissection testicular sperm extraction (micro-TESE) were enrolled in hCG-based hormonal therapy prior to a second micro-TESE.
In this study, hCG (human chorionic gonadotropin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking hCG (human chorionic gonadotropin).
Report a problemFND-2412391603
04HumanNot a test of hCG (human chorionic gonadotropin) aloneNon-randomized trialOther
In men with non-obstructive azoospermia, the sperm-positive group had lower basal ITT than the sperm-negative group.
In plain wordsIn men with azoospermia given hCG-based therapy, testicular testosterone before therapy was lower in men whose sperm retrieval succeeded.
Peptide
hCG (human chorionic gonadotropin)
Outcome
DecreaseBasal ITT by sperm retrieval status
Study
n = 20
Comparator
Sperm-negative group
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
p value for basal ITT by sperm retrieval status
< 0.05 p value
Sperm-positive versus sperm-negative group
Source sentences
Summary
The sperm-positive group showed a significantly lower basal ITT level compared with the sperm-negative group (p < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Twenty patients who failed sperm retrieval procedures using microdissection testicular sperm extraction (micro-TESE) were enrolled in hCG-based hormonal therapy prior to a second micro-TESE.
In this study, hCG (human chorionic gonadotropin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking hCG (human chorionic gonadotropin).
Report a problemFND-2412391604
05HumanNot a test of hCG (human chorionic gonadotropin) aloneNon-randomized trialOther
In men receiving hCG-based therapy for azoospermia, PCNA-LI increases were negatively associated with basal ITT.
In plain wordsIn men receiving hCG-based therapy for azoospermia, larger DNA synthesis marker increases were linked to lower baseline testicular testosterone.
Peptide
hCG (human chorionic gonadotropin)
Outcome
DecreasePCNA-LI increase association with basal ITT
Study
n = 20
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
p value for PCNA-LI increase association with basal ITT
< 0.05 p value
PCNA-LI increase versus basal ITT at initial micro-TESE
Source sentences
Summary
In addition, there was a significant negative association between the increase in PCNA-LI and basal ITT levels at the initial micro-TESE (p < 0.05), but not the stimulated ITT level at the second micro-TESE.
1 context sentence(design, population, supporting numbers)
Summary · context
Twenty patients who failed sperm retrieval procedures using microdissection testicular sperm extraction (micro-TESE) were enrolled in hCG-based hormonal therapy prior to a second micro-TESE.
In this study, hCG (human chorionic gonadotropin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking hCG (human chorionic gonadotropin).
Report a problemFND-2412391606
06HumanNot a test of hCG (human chorionic gonadotropin) aloneNon-randomized trialOther
In men receiving hCG-based therapy for azoospermia, PCNA-LI increases had no significant association with stimulated ITT.
In plain wordsIn men receiving hCG-based therapy for azoospermia, DNA synthesis marker increases were not significantly linked to stimulated testicular testosterone.
Peptide
hCG (human chorionic gonadotropin)
Outcome
No significant changePCNA-LI increase association with stimulated ITT
Study
n = 20
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
In addition, there was a significant negative association between the increase in PCNA-LI and basal ITT levels at the initial micro-TESE (p < 0.05), but not the stimulated ITT level at the second micro-TESE.
1 context sentence(design, population, supporting numbers)
Summary · context
Twenty patients who failed sperm retrieval procedures using microdissection testicular sperm extraction (micro-TESE) were enrolled in hCG-based hormonal therapy prior to a second micro-TESE.
In this study, hCG (human chorionic gonadotropin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking hCG (human chorionic gonadotropin).
Report a problemFND-2412391607
07HumanNot a test of hCG (human chorionic gonadotropin) aloneNon-randomized trialMechanism
In men with non-obstructive azoospermia receiving hCG-based therapy, PCNA-positive cells occurred predominantly in spermatogonia and primary spermatocytes.
In plain wordsIn men receiving hCG-based therapy for azoospermia, cells with the DNA synthesis marker were mainly spermatogonia and primary spermatocytes.
Peptide
hCG (human chorionic gonadotropin)
Outcome
Not applicablePCNA-positive cell localization
Study
n = 20
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
PCNA-positive cells were predominantly in the spermatogonia and primary spermatocytes, and PCNA-LI was significantly increased after the hormonal therapy.
1 context sentence(design, population, supporting numbers)
Summary · context
Twenty patients who failed sperm retrieval procedures using microdissection testicular sperm extraction (micro-TESE) were enrolled in hCG-based hormonal therapy prior to a second micro-TESE.
In this study, hCG (human chorionic gonadotropin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking hCG (human chorionic gonadotropin).
Report a problemFND-2412391608
08HumanNot a test of hCG (human chorionic gonadotropin) aloneNon-randomized trialEfficacy outcome
In men with non-obstructive azoospermia, combined recombinant FSH and hCG treatment markedly increased PCNA-LI.
In plain wordsIn men with non-obstructive azoospermia, combined FSH and hCG increased a marker of sperm-cell DNA synthesis.
Peptide
hCG (human chorionic gonadotropin)
Outcome
IncreasePCNA-LI with combined treatment
Study
n: not reported in source
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
There was a marked increase in the PCNA-LI levels of men treated with both recombinant human follicle-stimulating hormone and hCG.
1 context sentence(design, population, supporting numbers)
Summary · context
Twenty patients who failed sperm retrieval procedures using microdissection testicular sperm extraction (micro-TESE) were enrolled in hCG-based hormonal therapy prior to a second micro-TESE.
In this study, hCG (human chorionic gonadotropin) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking hCG (human chorionic gonadotropin).
Report a problemFND-2412391605

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
14 Oct 2026, 05:02 UTC
Study results updated
Version 1
14 Oct 2026, 01:46 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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