Possible involvement of AMPK in acute exercise-induced expression of monocarboxylate transporters MCT1 and MCT4 mRNA in fast-twitch skeletal muscle.

Takimoto M, Takeyama M, Hamada T

Metabolism: clinical and experimental23 Jul 2013Cited by 29 (Europe PMC)

PMID23886299DOI10.1016/j.metabol.2013.06.010PepBankPUB-23886299

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

3 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueMechanism
In isolated rat epitrochlearis muscle, direct AICAR exposure activated phosphorylated AMPK.
In plain wordsIn lab tests with rat muscle, AICAR activated phosphorylated AMPK.
Peptide
AICAR (acadesine)
Outcome
IncreaseAMPK phosphorylation
Study
ratn: not reported in source
Isolated epitrochlearis muscle
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
0.5 mmol/LAs given in this study (rat).
Source sentences
Summary
Direct exposure of the epitrochlearis muscle to 0.5mmol/L AICAR or 1mmol/L caffeine, which activated p-AMPK increased both MCT1 and MCT4 mRNA levels.
1 context sentence(design, population, supporting numbers)
Summary · context
To observe the direct effects of AMPK activation on MCTs mRNA, the effects of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR), caffeine, and dantrolene were analyzed in vitro using an isolated muscle incubation model.
Report a problemFND-2388629901
02LabEx vivo tissueMechanism
In isolated rat epitrochlearis muscle, direct AICAR exposure increased MCT1 mRNA levels.
In plain wordsIn lab tests with rat muscle, AICAR increased MCT1 mRNA levels.
Peptide
AICAR (acadesine)
Outcome
IncreaseMCT1 mRNA
Study
ratn: not reported in source
Isolated epitrochlearis muscle
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
0.5 mmol/LAs given in this study (rat).
Source sentences
Summary
Direct exposure of the epitrochlearis muscle to 0.5mmol/L AICAR or 1mmol/L caffeine, which activated p-AMPK increased both MCT1 and MCT4 mRNA levels.
1 context sentence(design, population, supporting numbers)
Summary · context
To observe the direct effects of AMPK activation on MCTs mRNA, the effects of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR), caffeine, and dantrolene were analyzed in vitro using an isolated muscle incubation model.
Report a problemFND-2388629902
03LabEx vivo tissueMechanism
In isolated rat epitrochlearis muscle, direct AICAR exposure increased MCT4 mRNA levels.
In plain wordsIn lab tests with rat muscle, AICAR increased MCT4 mRNA levels.
Peptide
AICAR (acadesine)
Outcome
IncreaseMCT4 mRNA
Study
ratn: not reported in source
Isolated epitrochlearis muscle
Outcome group
Receptor activity and mechanism · Musculoskeletal
Studied dose
0.5 mmol/LAs given in this study (rat).
Source sentences
Summary
Direct exposure of the epitrochlearis muscle to 0.5mmol/L AICAR or 1mmol/L caffeine, which activated p-AMPK increased both MCT1 and MCT4 mRNA levels.
1 context sentence(design, population, supporting numbers)
Summary · context
To observe the direct effects of AMPK activation on MCTs mRNA, the effects of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR), caffeine, and dantrolene were analyzed in vitro using an isolated muscle incubation model.
Report a problemFND-2388629903

Studies that did not test the peptide alone2

In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking AICAR (acadesine).

04LabNot a test of AICAR (acadesine) aloneEx vivo tissueMechanism
In isolated rat muscle, MCT1 mRNA did not increase when dantrolene inhibited phosphorylated AMPK during the activator experiment.
In plain wordsIn lab tests with rat muscle, MCT1 mRNA did not increase when dantrolene blocked phosphorylated AMPK.
Peptide
AICAR (acadesine)
Outcome
No significant changeMCT1 mRNA with dantrolene
Study
ratn: not reported in source
Isolated epitrochlearis muscle
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
When pAMPK was inhibited by dantrolene, neither MCT1 nor MCT4 mRNA was increased.
2 context sentences(design, population, supporting numbers)
Summary · context
Direct exposure of the epitrochlearis muscle to 0.5mmol/L AICAR or 1mmol/L caffeine, which activated p-AMPK increased both MCT1 and MCT4 mRNA levels.
Summary · context
To observe the direct effects of AMPK activation on MCTs mRNA, the effects of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR), caffeine, and dantrolene were analyzed in vitro using an isolated muscle incubation model.
In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AICAR (acadesine).
Report a problemFND-2388629904
05LabNot a test of AICAR (acadesine) aloneEx vivo tissueMechanism
In isolated rat muscle, MCT4 mRNA did not increase when dantrolene inhibited phosphorylated AMPK during the activator experiment.
In plain wordsIn lab tests with rat muscle, MCT4 mRNA did not increase when dantrolene blocked phosphorylated AMPK.
Peptide
AICAR (acadesine)
Outcome
No significant changeMCT4 mRNA with dantrolene
Study
ratn: not reported in source
Isolated epitrochlearis muscle
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
When pAMPK was inhibited by dantrolene, neither MCT1 nor MCT4 mRNA was increased.
2 context sentences(design, population, supporting numbers)
Summary · context
Direct exposure of the epitrochlearis muscle to 0.5mmol/L AICAR or 1mmol/L caffeine, which activated p-AMPK increased both MCT1 and MCT4 mRNA levels.
Summary · context
To observe the direct effects of AMPK activation on MCTs mRNA, the effects of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR), caffeine, and dantrolene were analyzed in vitro using an isolated muscle incubation model.
In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AICAR (acadesine).
Report a problemFND-2388629905

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

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Terms as the source lists them.

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