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Study · In vitro (cells) · rat · 2013

6 results

Potassium Current Is Not Affected by Long-Term Exposure to Ghrelin or GHRP-6 in Somatotropes GC Cells.

Domínguez Mancera B, Monjaraz Guzman E, Flores-Hernández JL, Barrientos Morales M, Martínez Hernandez JM, Hernández Beltran A, Cervantes Acosta P

Journal of biophysics (Hindawi Publishing Corporation : Online)24 Feb 2013Cited by 1 (Europe PMC)

PMID23533398 (opens in a new tab)PMCIDPMC3600309 (opens in a new tab)DOI10.1155/2013/913792 (opens in a new tab)PepBankPUB-23533398
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Summary

Ghrelin is a growth hormone (GH) secretagogue (GHS) and GHRP-6 is a synthetic peptide analogue; both act through the GHS receptor. GH secretion depends directly on the intracellular concentration of Ca(2+); this is determined from the intracellular reserves and by the entrance of Ca(2+) through the voltage-dependent calcium channels, which are activated by the membrane depolarization. Membrane potential is mainly determined by K(+) channels. In the present work, we investigated the effect of ghrelin (10 nM) or GHRP-6 (100 nM) for 96 h on functional expression of voltage-dependent K(+) channels in rat somatotropes: GC cell line. Physiological patch-clamp whole-cell recording was used to register the K(+) currents. With Cd(2+) (1 mM) and tetrodotoxin (1  μ m) in the bath solution recording, three types of currents were characterized on the basis of their biophysical and pharmacological properties. GC cells showed a K(+) current with a transitory component (I A) sensitive to 4-aminopyridine, which represents ~40% of the total outgoing current; a sustained component named delayed rectifier (I K), sensitive to tetraethylammonium; and a third type of K(+) current was recorded at potentials more negative than -80 mV, permitting the entrance of K(+) named inward rectifier (KIR). Chronic treatment with ghrelin or GHRP-6 did not modify the functional expression of K(+) channels, without significant changes (P < 0.05) in the amplitudes of the three currents observed; in addition, there were no modifications in their biophysical properties and kinetic activation or inactivation.
CC BYText from Europe PMC. Highlighted sentences are the source of results.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In rat GC cells, chronic GHRP‑6 treatment did not significantly change transient potassium current amplitude.

    In plain wordsIn cells from rats, GHRP‑6 did not significantly change transient potassium current amplitude.

    Peptide
    GHRP-6
    Outcome
    No significant changeTransient potassium current amplitude
    Study

    ratn: not reported in source

    GC somatotrope cell line
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    100 nM · 96 hAs given in this study (rat).

    Source sentences

    Summary
    Chronic treatment with ghrelin or GHRP-6 did not modify the functional expression of K(+) channels, without significant changes (P < 0.05) in the amplitudes of the three currents observed; in addition, there were no modifications in their biophysical properties and kinetic activation or inactivation.
    2 context sentences
    Summary · context
    GC cells showed a K(+) current with a transitory component (I A) sensitive to 4-aminopyridine, which represents ~40% of the total outgoing current; a sustained component named delayed rectifier (I K), sensitive to tetraethylammonium;
    Summary · context
    In the present work, we investigated the effect of ghrelin (10 nM) or GHRP-6 (100 nM) for 96 h on functional expression of voltage-dependent K(+) channels in rat somatotropes: GC cell line.
  2. 02LabIn vitro (cells)Activity measurement

    In rat GC cells, chronic GHRP‑6 treatment did not significantly change delayed rectifier potassium current amplitude.

    In plain wordsIn cells from rats, GHRP‑6 did not significantly change delayed rectifier potassium current amplitude.

    Peptide
    GHRP-6
    Outcome
    No significant changeDelayed rectifier potassium current amplitude
    Study

    ratn: not reported in source

    GC somatotrope cell line
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    100 nM · 96 hAs given in this study (rat).

    Source sentences

    Summary
    Chronic treatment with ghrelin or GHRP-6 did not modify the functional expression of K(+) channels, without significant changes (P < 0.05) in the amplitudes of the three currents observed; in addition, there were no modifications in their biophysical properties and kinetic activation or inactivation.
    2 context sentences
    Summary · context
    GC cells showed a K(+) current with a transitory component (I A) sensitive to 4-aminopyridine, which represents ~40% of the total outgoing current; a sustained component named delayed rectifier (I K), sensitive to tetraethylammonium;
    Summary · context
    In the present work, we investigated the effect of ghrelin (10 nM) or GHRP-6 (100 nM) for 96 h on functional expression of voltage-dependent K(+) channels in rat somatotropes: GC cell line.
  3. 03LabIn vitro (cells)Activity measurement

    In rat GC cells, chronic GHRP‑6 treatment did not significantly change inward rectifier potassium current amplitude.

    In plain wordsIn cells from rats, GHRP‑6 did not significantly change inward rectifier potassium current amplitude.

    Peptide
    GHRP-6
    Outcome
    No significant changeInward rectifier potassium current amplitude
    Study

    ratn: not reported in source

    GC somatotrope cell line
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    100 nM · 96 hAs given in this study (rat).

    Source sentences

    Summary
    Chronic treatment with ghrelin or GHRP-6 did not modify the functional expression of K(+) channels, without significant changes (P < 0.05) in the amplitudes of the three currents observed; in addition, there were no modifications in their biophysical properties and kinetic activation or inactivation.
    2 context sentences
    Summary · context
    and a third type of K(+) current was recorded at potentials more negative than -80 mV, permitting the entrance of K(+) named inward rectifier (KIR).
    Summary · context
    In the present work, we investigated the effect of ghrelin (10 nM) or GHRP-6 (100 nM) for 96 h on functional expression of voltage-dependent K(+) channels in rat somatotropes: GC cell line.
  4. 04LabIn vitro (cells)Activity measurement

    In rat GC cells, chronic GHRP‑6 treatment did not modify potassium channel biophysical properties.

    In plain wordsIn cells from rats, GHRP‑6 did not change potassium channel biophysical properties.

    Peptide
    GHRP-6
    Outcome
    No significant changePotassium channel biophysical properties
    Study

    ratn: not reported in source

    GC somatotrope cell line
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    100 nM · 96 hAs given in this study (rat).

    Source sentences

    Summary
    in addition, there were no modifications in their biophysical properties and kinetic activation or inactivation.
    1 context sentence
    Summary · context
    In the present work, we investigated the effect of ghrelin (10 nM) or GHRP-6 (100 nM) for 96 h on functional expression of voltage-dependent K(+) channels in rat somatotropes: GC cell line.
  5. 05LabIn vitro (cells)Activity measurement

    In rat GC cells, chronic GHRP‑6 treatment did not modify potassium channel activation kinetics.

    In plain wordsIn cells from rats, GHRP‑6 did not change how quickly potassium channels activated.

    Peptide
    GHRP-6
    Outcome
    No significant changePotassium channel activation kinetics
    Study

    ratn: not reported in source

    GC somatotrope cell line
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    100 nM · 96 hAs given in this study (rat).

    Source sentences

    Summary
    in addition, there were no modifications in their biophysical properties and kinetic activation or inactivation.
    1 context sentence
    Summary · context
    In the present work, we investigated the effect of ghrelin (10 nM) or GHRP-6 (100 nM) for 96 h on functional expression of voltage-dependent K(+) channels in rat somatotropes: GC cell line.
  6. 06LabIn vitro (cells)Activity measurement

    In rat GC cells, chronic GHRP‑6 treatment did not modify potassium channel inactivation kinetics.

    In plain wordsIn cells from rats, GHRP‑6 did not change how quickly potassium channels inactivated.

    Peptide
    GHRP-6
    Outcome
    No significant changePotassium channel inactivation kinetics
    Study

    ratn: not reported in source

    GC somatotrope cell line
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    100 nM · 96 hAs given in this study (rat).

    Source sentences

    Summary
    in addition, there were no modifications in their biophysical properties and kinetic activation or inactivation.
    1 context sentence
    Summary · context
    In the present work, we investigated the effect of ghrelin (10 nM) or GHRP-6 (100 nM) for 96 h on functional expression of voltage-dependent K(+) channels in rat somatotropes: GC cell line.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:13 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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