Skip to content

Study · In vitro (cells) · rat · 2013

15 results

Effects of AMPK activation and combined treatment with AMPK activators and somatostatin on hormone secretion and cell growth in cultured GH-secreting pituitary tumor cells.

Tulipano G, Faggi L, Losa M, Mortini P, Spinello M, Sibilia V, Pagani F, Cocchi D, Giustina A

Molecular and cellular endocrinology29 Oct 2012Cited by 11 (Europe PMC)

PMID23116772 (opens in a new tab)DOI10.1016/j.mce.2012.10.017 (opens in a new tab)PepBankPUB-23116772
Publisher pagePubMedAsk about this study
Jump to 15 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study15

9 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In cultured human pituitary adenomas, four of 15 adenomas showed a cell viability response to AICAR.

    In plain wordsIn cells from human pituitary tumors, four of 15 tumors showed a cell viability response to AICAR.

    Peptide
    AICAR (acadesine)
    Outcome
    Direction not reportedCell viability response
    Study

    humann = 15

    cultured human GH-secreting pituitary adenomas
    Outcome group
    Tumor growth · Endocrine and hormones
    Studied dose
    0.4mMAs given in this study (human).

    Measurements

    Responsive adenomas
    4 adenomas
    AICAR cell viability response

    Source sentences

    Summary
    As to the effects on cell viability, four adenomas out of 15 were responsive to AICAR (0.4mM) and five adenomas were responsive to SS-14 (100 nM).
  2. 02LabIn vitro (cells)Mechanism

    In cultured human pituitary adenomas, one adenoma responded to both separately tested AICAR and somatostatin.

    In plain wordsIn cells from human pituitary tumors, one tumor responded to both AICAR and somatostatin tested separately.

    Peptide
    AICAR (acadesine)
    Outcome
    Direction not reportedResponse to both agents for cell viability
    Study

    humann = 15

    cultured human GH-secreting pituitary adenomas
    Outcome group
    Tumor growth · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    One adenoma was responsive to both somatostatin and AICAR.
    1 context sentence
    Summary · context
    As to the effects on cell viability, four adenomas out of 15 were responsive to AICAR (0.4mM) and five adenomas were responsive to SS-14 (100 nM).
  3. 03LabIn vitro (cells)Mechanism

    In cultured human pituitary adenomas, nine of 15 adenomas showed a GH secretion response to AICAR.

    In plain wordsIn cells from human pituitary tumors, nine of 15 tumors showed a growth hormone secretion response to AICAR.

    Peptide
    AICAR (acadesine)
    Outcome
    Direction not reportedGH secretion response
    Study

    humann = 15

    cultured human GH-secreting pituitary adenomas
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    As to the effects on GH secretion, nine adenomas out of 15 were responsive to AICAR.
  4. 04LabIn vitro (cells)Mechanism

    In cultured human pituitary adenomas, eight adenomas responded to both separately tested AICAR and SS-14 for GH secretion.

    In plain wordsIn cells from eight human pituitary tumors, growth hormone secretion responded to both AICAR and SS-14 tested separately.

    Peptide
    AICAR (acadesine)
    Outcome
    Direction not reportedGH secretion response to both agents
    Study

    humann = 15

    cultured human GH-secreting pituitary adenomas
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Eight adenomas were responsive to both AICAR and SS-14.
    1 context sentence
    Summary · context
    As to the effects on GH secretion, nine adenomas out of 15 were responsive to AICAR.
  5. 05LabIn vitro (cells)Mechanism

    In cultured human pituitary adenomas, AICAR plus SS-14 exceeded SS-14 alone for GH secretion effects in 4 of 10 adenomas.

    In plain wordsIn cells from human pituitary tumors, adding AICAR exceeded SS-14 alone for growth hormone secretion effects in 4 of 10 tumors.

    Peptide
    AICAR (acadesine)
    Outcome
    Direction not reportedCombination GH secretion effect versus SS-14
    Study

    humann = 10

    cultured human GH-secreting pituitary adenomas
    Comparator
    SS-14 alone
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Cotreatment exceeded the effect of single treatments in 4 out of 10 adenomas.
    1 context sentence
    Summary · context
    As to the effects on GH secretion, nine adenomas out of 15 were responsive to AICAR.
  6. 06LabIn vitro (cells)Mechanism

    In cultured human pituitary adenomas, overnight AICAR treatment increased phospho-AMPK levels.

    In plain wordsIn cells from human pituitary tumors, overnight AICAR increased AMPK activation.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseAMPK phosphorylation
    Study

    humann: not reported in source

    cultured human GH-secreting pituitary adenomas
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated · overnightAs given in this study (human).

    Source sentences

    Summary
    Overnight treatment with AICAR increased phospho-(threonine-172) AMPK levels (activated AMPK) in cultured human adenomas.
  7. 07LabIn vitro (cells)Mechanism

    In rat GH3 cells, AICAR reduced p70S6 kinase activity.

    In plain wordsIn cells from rat pituitary tumors, AICAR reduced p70S6 kinase activity.

    Peptide
    AICAR (acadesine)
    Outcome
    Decreasep70S6 kinase activity
    Study

    ratn: not reported in source

    GH3 cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In GH3 cells, AICAR reduced the activity of p70S6 kinase, which plays an important role in cell growth.
  8. 08LabIn vitro (cells)Mechanism

    In rat GH3 cells, AICAR reduced ERK phosphorylation with a time course different from SS-14.

    In plain wordsIn cells from rat pituitary tumors, AICAR reduced ERK phosphorylation with a different time course from SS-14.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseERK phosphorylation
    Study

    ratn: not reported in source

    GH3 cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, AICAR and SS-14 reduced ERK phosphorylation with a different time course.
    1 context sentence
    Summary · context
    In GH3 cells, AICAR reduced the activity of p70S6 kinase, which plays an important role in cell growth.
  9. 09LabIn vitro (cells)Mechanism

    In rat GH3 cells, AICAR inhibited phospho-S6 levels.

    In plain wordsIn cells from rat pituitary tumors, AICAR reduced phospho-S6 levels.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreasePhospho-S6 levels
    Study

    ratn: not reported in source

    GH3 cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    SS-14 did not affect significantly AMPK phosphorylation and p70S6K activity but it was able to enhance the inhibitory effect of AICAR on phospho-S6 levels.
    1 context sentence
    Summary · context
    In GH3 cells, AICAR reduced the activity of p70S6 kinase, which plays an important role in cell growth.

Studies that did not test the peptide alone6

In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking AICAR (acadesine).

  1. 10LabNot a test of AICAR (acadesine) aloneIn vitro (cells)Mechanism

    In cultured human pituitary adenomas, AICAR plus SS-14 exceeded AICAR alone for GH secretion effects in 4 of 10 adenomas.

    In plain wordsIn cells from human pituitary tumors, AICAR plus SS-14 exceeded AICAR alone for growth hormone secretion effects in 4 of 10 tumors.

    Peptide
    AICAR (acadesine)
    Outcome
    Direction not reportedCombination GH secretion effect versus AICAR
    Study

    humann = 10

    cultured human GH-secreting pituitary adenomas
    Comparator
    AICAR alone
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    Cotreatment exceeded the effect of single treatments in 4 out of 10 adenomas.
    1 context sentence
    Summary · context
    As to the effects on GH secretion, nine adenomas out of 15 were responsive to AICAR.

    In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AICAR (acadesine).

  2. 11LabNot a test of AICAR (acadesine) aloneIn vitro (cells)Mechanism

    In two human pituitary adenomas, AICAR plus SS-14 did not exceed AICAR alone in its cell viability effect.

    In plain wordsIn cells from two human pituitary tumors, AICAR plus SS-14 did not exceed AICAR alone in its cell viability effect.

    Peptide
    AICAR (acadesine)
    Outcome
    No significant changeCombination cell viability effect versus AICAR
    Study

    humann = 2

    cultured human GH-secreting pituitary adenomas
    Comparator
    AICAR alone
    Outcome group
    Tumor growth · Endocrine and hormones

    Source sentences

    Summary
    In two adenomas, the effects of AICAR+SS-14 did not exceed the effect of AICAR.

    In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AICAR (acadesine).

  3. 12LabNot a test of AICAR (acadesine) aloneIn vitro (cells)Mechanism

    In two human pituitary adenomas unresponsive to either agent alone, AICAR plus SS-14 reduced cell viability versus control.

    In plain wordsIn cells from two human pituitary tumors, AICAR plus SS-14 reduced cell viability versus control when either agent alone failed.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseCombination cell viability versus control
    Study

    humann = 2

    cultured human GH-secreting pituitary adenomas
    Comparator
    control
    Outcome group
    Tumor growth · Endocrine and hormones

    Source sentences

    Summary
    In two adenomas which were not responsive to either AICAR or SS-14, the cotreatment was able to reduce cell viability versus control.

    In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AICAR (acadesine).

  4. 13LabNot a test of AICAR (acadesine) aloneIn vitro (cells)Mechanism

    In cultured human pituitary adenomas, two adenomas did not respond to any treatment, including AICAR alone and AICAR plus SS-14.

    In plain wordsIn cells from two human pituitary tumors, no treatment produced a cell viability response.

    Peptide
    AICAR (acadesine)
    Outcome
    No significant changeCell viability nonresponse to all treatments
    Study

    humann = 2

    cultured human GH-secreting pituitary adenomas
    Outcome group
    Tumor growth · Endocrine and hormones

    Source sentences

    Summary
    Two adenomas were not responsive to any treatment.
    1 context sentence
    Summary · context
    The effects of cotreatment with SS-14 and AICAR were investigated in eight adenomas.

    In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AICAR (acadesine).

  5. 14LabNot a test of AICAR (acadesine) aloneIn vitro (cells)Mechanism

    In rat GH3 cells, SS-14 enhanced the inhibitory effect of AICAR on phospho-S6 levels.

    In plain wordsIn cells from rat pituitary tumors, adding SS-14 to AICAR reduced phospho-S6 more than AICAR alone.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreasePhospho-S6 levels with SS-14 addition
    Study

    ratn: not reported in source

    GH3 cells
    Comparator
    AICAR alone
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Source sentences

    Summary
    SS-14 did not affect significantly AMPK phosphorylation and p70S6K activity but it was able to enhance the inhibitory effect of AICAR on phospho-S6 levels.
    1 context sentence
    Summary · context
    In GH3 cells, AICAR reduced the activity of p70S6 kinase, which plays an important role in cell growth.

    In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AICAR (acadesine).

  6. 15LabNot a test of AICAR (acadesine) aloneIn vitro (cells)Mechanism

    In rat GH3 cells, combined AICAR and SS-14 reduced phospho-ERK levels at every measured time point.

    In plain wordsIn cells from rat pituitary tumors, AICAR plus SS-14 reduced phospho-ERK at every measured time point.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseCombination phospho-ERK levels
    Study

    ratn: not reported in source

    GH3 cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Source sentences

    Summary
    The combined treatment reduced phospho-ERK levels at any time point.
    1 context sentence
    Summary · context
    In GH3 cells, AICAR reduced the activity of p70S6 kinase, which plays an important role in cell growth.

    In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AICAR (acadesine).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.