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Study · In vitro (cells) · 2012

5 results

The nuclear localization of MGF receptor in osteoblasts under mechanical stimulation.

Peng Q, Qiu J, Sun J, Yang L, Zhang B, Wang Y

Molecular and cellular biochemistry3 Jul 2012Cited by 8 (Europe PMC)

PMID22752413 (opens in a new tab)DOI10.1007/s11010-012-1377-9 (opens in a new tab)PepBankPUB-22752413
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In primary osteoblasts, fluorescence intensity of internalized FITC-MGF increased with the concentration of FITC-MGF.

    In plain wordsIn cells, more labeled MGF in the dish meant more MGF taken up by bone cells.

    Peptide
    MGF / PEG-MGF
    Outcome
    IncreaseFluorescence intensity of internalized FITC-MGF
    Study

    n: not reported in source

    Primary osteoblasts
    Comparator
    Lower FITC-MGF concentrations
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    0.01, 0.1 and 1 mg/ml FITC-MGFAs given in this study.

    Source sentences

    Summary
    Our results showed that the fluorescence intensity and cell population internalizing FITC-MGF increased with the concentration of FITC-MGF.
    1 context sentence
    Summary · context
    After the primary osteoblasts were exposed to stretch with the strain at 10 %, and/or loaded with 50 ng/ml exogenous MGF-Ct24E, cells were incubated with the different concentrations of FITC-MGF (0.01, 0.1, and 1 mg/ml) followed by flow cytometry and laser scanning confocal microscope analysis.
  2. 02LabIn vitro (cells)Mechanism

    In primary osteoblasts, the cell population internalizing FITC-MGF increased with the concentration of FITC-MGF.

    In plain wordsIn cells, more labeled MGF in the dish meant more bone cells took it up.

    Peptide
    MGF / PEG-MGF
    Outcome
    IncreaseCell population internalizing FITC-MGF
    Study

    n: not reported in source

    Primary osteoblasts
    Comparator
    Lower FITC-MGF concentrations
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    0.01, 0.1 and 1 mg/ml FITC-MGFAs given in this study.

    Source sentences

    Summary
    Our results showed that the fluorescence intensity and cell population internalizing FITC-MGF increased with the concentration of FITC-MGF.
    1 context sentence
    Summary · context
    After the primary osteoblasts were exposed to stretch with the strain at 10 %, and/or loaded with 50 ng/ml exogenous MGF-Ct24E, cells were incubated with the different concentrations of FITC-MGF (0.01, 0.1, and 1 mg/ml) followed by flow cytometry and laser scanning confocal microscope analysis.
  3. 03LabIn vitro (cells)Mechanism

    In primary osteoblasts, all the cells were labeled with fluorescence at 1 mg/ml FITC-MGF.

    In plain wordsIn cells, all bone cells took up labeled MGF at the highest concentration.

    Peptide
    MGF / PEG-MGF
    Outcome
    Not applicableProportion of cells labeled at 1 mg/ml
    Study

    n: not reported in source

    Primary osteoblasts
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    1 mg/ml FITC-MGFAs given in this study.

    Measurements

    FITC-MGF concentration
    1 mg/ml
    all cells labeled

    Source sentences

    Summary
    And all the cells were labeled with fluorescence at 1 mg/ml.
    1 context sentence
    Summary · context
    After the primary osteoblasts were exposed to stretch with the strain at 10 %, and/or loaded with 50 ng/ml exogenous MGF-Ct24E, cells were incubated with the different concentrations of FITC-MGF (0.01, 0.1, and 1 mg/ml) followed by flow cytometry and laser scanning confocal microscope analysis.
  4. 04LabIn vitro (cells)Mechanism

    In primary osteoblasts, FITC-MGF had nuclear localization under stretch and/or 50 ng/ml MGF-Ct24E, compared with cytoplasmic localization in the static culture group.

    In plain wordsIn cells, labeled MGF moved into the nucleus of bone cells under stretch or added MGF, but stayed outside it in resting cells.

    Peptide
    MGF / PEG-MGF
    Outcome
    Not applicableSubcellular localization of FITC-MGF under stretch or MGF-Ct24E
    Study

    n: not reported in source

    Primary osteoblasts
    Comparator
    Static culture group
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    0.01, 0.1 and 1 mg/ml FITC-MGFAs given in this study.

    Source sentences

    Summary
    Notably, FITC-MGF had nuclear localization when osteoblasts were exposed to stretch and/or 50 ng/ml MGF-Ct24E added, compared to the evident cytoplasmic localization in the static culture group.
    1 context sentence
    Summary · context
    After the primary osteoblasts were exposed to stretch with the strain at 10 %, and/or loaded with 50 ng/ml exogenous MGF-Ct24E, cells were incubated with the different concentrations of FITC-MGF (0.01, 0.1, and 1 mg/ml) followed by flow cytometry and laser scanning confocal microscope analysis.
  5. 05LabIn vitro (cells)Mechanism

    In primary osteoblasts, nuclear localization of FITC-MGF in response to mechanical loading was associated with high expression of proliferating cell nuclear antigen.

    In plain wordsIn cells, MGF moving into the nucleus under stretch was linked to higher levels of a cell growth marker (PCNA).

    Peptide
    MGF / PEG-MGF
    Outcome
    IncreaseFITC-MGF nuclear localization and PCNA expression
    Study

    n: not reported in source

    Primary osteoblasts
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    0.01, 0.1 and 1 mg/ml FITC-MGFAs given in this study.

    Source sentences

    Summary
    The nuclear localization of FITC-MGF in response to mechanical loading was found to associate with high expression of proliferating cell nuclear antigen, suggesting MGF and its receptor could serve as potential messengers that replay information in nuclei to control cell proliferation.
    1 context sentence
    Summary · context
    After the primary osteoblasts were exposed to stretch with the strain at 10 %, and/or loaded with 50 ng/ml exogenous MGF-Ct24E, cells were incubated with the different concentrations of FITC-MGF (0.01, 0.1, and 1 mg/ml) followed by flow cytometry and laser scanning confocal microscope analysis.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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