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Study · Not a test of the peptide alone · 2012

3 results

Intein-mediated expression, purification, and characterization of thymosin α1-thymopentin fusion peptide in Escherichia coli.

Li J, Zheng L, Li P, Wang F

Protein expression and purification25 Apr 2012Cited by 8 (Europe PMC)

PMID22554820 (opens in a new tab)DOI10.1016/j.pep.2012.04.013 (opens in a new tab)PepBankPUB-22554820
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone3

In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin alpha-1.

  1. 01LabNot a test of Thymosin alpha-1 aloneIn vitro (cells)Activity measurement

    In vitro, the purified Tα1-TP5 fusion peptide stimulated proliferation of mouse splenic lymphocytes.

    In plain wordsIn lab tests, the thymosin α1 fusion peptide stimulated the growth of mouse spleen immune cells.

    Peptide
    Thymosin alpha-1
    Outcome
    IncreaseProliferation of mouse splenic lymphocytes
    Study

    mousen: not reported in source

    Splenic lymphocytes
    Outcome group
    Immune response · Immune and inflammation

    Source sentences

    Summary
    In vitro, the purified Tα1-TP5 could stimulate the proliferation of mouse splenic lymphocytes.
    1 context sentence
    Summary · context
    To obtain Tα1-TP5 more effectively and economically, Tα1-TP5 was genetically fused to a self-cleaving intein-chitin binding domain tag for purification via chitin beads in Escherichia coli.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

  2. 02LabNot a test of Thymosin alpha-1 aloneOther designOther

    About 7.6 mg of Tα1-TP5 fusion peptide was obtained from 1 L of E. coli culture medium.

    In plain wordsIn lab production, a bacterial culture gave about 7.6 mg of the thymosin α1 fusion peptide per litre.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableYield of purified Tα1-TP5 from E. coli culture
    Study

    n: not reported in source

    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    yield
    ≈ 7.6 mg
    per 1 L culture media

    Source sentences

    Summary
    Finally, about 7.6 mg Tα1-TP5 purified from the soluble fraction and inclusion bodies was obtained from 1 L culture media.
    1 context sentence
    Summary · context
    To obtain Tα1-TP5 more effectively and economically, Tα1-TP5 was genetically fused to a self-cleaving intein-chitin binding domain tag for purification via chitin beads in Escherichia coli.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

  3. 03LabNot a test of Thymosin alpha-1 aloneOther designOther

    The Tα1-TP5 fusion peptide reached 95% purity after a series of chromatographic purification steps.

    In plain wordsIn lab production, the thymosin α1 fusion peptide reached 95% purity.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicablePurity of Tα1-TP5 after chromatography
    Study

    n: not reported in source

    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    purity
    95%
    after chromatographic purification

    Source sentences

    Summary
    The purity was 95% after a series of chromatographic purification steps.
    1 context sentence
    Summary · context
    To obtain Tα1-TP5 more effectively and economically, Tα1-TP5 was genetically fused to a self-cleaving intein-chitin binding domain tag for purification via chitin beads in Escherichia coli.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    10 Oct 2026, 08:55 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 03:02 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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