Na+ transport across rumen epithelium of hay-fed sheep is acutely stimulated by the peptide IGF-1 in vitro.

Shen Z, Martens H, Schweigel-Röntgen M

Experimental physiology6 Jan 2012Cited by 8 (Europe PMC)

PMID22227200DOI10.1113/expphysiol.2011.061580PepBankPUB-22227200

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

4 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueEfficacy outcome
In isolated rumen epithelium of hay-fed sheep, serosal LR3-IGF-1 rapidly increased mucosal-to-serosal and net Na+ flux.
In plain wordsIn lab tests on sheep rumen tissue, LR3-IGF-1 quickly increased sodium movement across the tissue.
Peptide
IGF-1 LR3
Outcome
IncreaseMucosal-to-serosal and net Na+ flux
Study
sheepn: not reported in source
isolated rumen epithelium of hay-fed sheep
Comparator
Controls
Outcome group
Electrolyte balance · Gastrointestinal
Studied dose
20 to 100 ug/l · serosal application · within 30 minAs given in this study (sheep).
Measurements
LR3-IGF-1 concentration range
20 to 100 μg l(-1)
serosal
Source sentences
Summary
At concentrations ranging from 20 to 100 μg l(-1), serosal LR3-IGF-1, a recombinant analogue of IGF-1, rapidly (within 30 min) stimulated the mucosal-to-serosal Na(+) flux (J(ms)Na) and consequently the net Na(+) flux (J(net)Na).
1 context sentence(design, population, supporting numbers)
Summary · context
This study examined the in vitro effect of IGF-1 on Na(+) transport across the rumen epithelium of hay-fed sheep, in which the IGF-1 concentration in plasma is lower than in concentrate-fed animals.
Report a problemFND-2222720001
02LabEx vivo tissueEfficacy outcome
In isolated rumen epithelium of hay-fed sheep, serosal LR3-IGF-1 at 20 ug/l raised net Na+ flux by about 60% versus controls.
In plain wordsIn lab tests on sheep rumen tissue, LR3-IGF-1 raised net sodium flux by about 60% compared with controls.
Peptide
IGF-1 LR3
Outcome
IncreaseNet Na+ flux (J(net)Na) at 20 ug/l
Study
sheepn: not reported in source
isolated rumen epithelium of hay-fed sheep
Comparator
Controls
Outcome group
Electrolyte balance · Gastrointestinal
Studied dose
20 ug/l · serosal applicationAs given in this study (sheep).
Measurements
J(net)Na increase
≈ 60%
LR3-IGF-1 20 μg l(-1)
Source sentences
Summary
Compared with controls, J(net)Na increased by about 60% (P < 0.05) following the serosal application of LR3-IGF-1 (20 μg l(-1)).
1 context sentence(design, population, supporting numbers)
Summary · context
This study examined the in vitro effect of IGF-1 on Na(+) transport across the rumen epithelium of hay-fed sheep, in which the IGF-1 concentration in plasma is lower than in concentrate-fed animals.
Report a problemFND-2222720002
03LabEx vivo tissueEfficacy outcome
In isolated rumen epithelium of hay-fed sheep, IGF-1 did not alter tissue conductance or short-circuit current.
In plain wordsIn lab tests on sheep rumen tissue, IGF-1 did not change tissue conductance or short-circuit current.
Peptide
IGF-1 LR3
Outcome
No significant changeTissue conductance and short-circuit current
Study
sheepn: not reported in source
isolated rumen epithelium of hay-fed sheep
Comparator
Controls
Outcome group
Electrolyte balance · Gastrointestinal
Studied dose
Dose not stated · serosal applicationAs given in this study (sheep).
Source sentences
Summary
Neither IGF-1 nor amiloride altered tissue conductance or the short-circuit current of the isolated rumen epithelium.
1 context sentence(design, population, supporting numbers)
Summary · context
This study examined the in vitro effect of IGF-1 on Na(+) transport across the rumen epithelium of hay-fed sheep, in which the IGF-1 concentration in plasma is lower than in concentrate-fed animals.
Report a problemFND-2222720004
04LabIn vitro (cells)Efficacy outcome
In cultured sheep rumen epithelial cells, LR3-IGF-1 at 20 ug/l significantly increased the rate of intracellular pH recovery after acid loading.
In plain wordsIn sheep rumen cells grown in the lab, LR3-IGF-1 sped up recovery from acid loading.
Peptide
IGF-1 LR3
Outcome
IncreaseRate of intracellular pH recovery after acid loading
Study
sheepn: not reported in source
cultured rumen epithelial cells
Comparator
Control cells
Outcome group
Electrolyte balance · Gastrointestinal
Studied dose
20 ug/lAs given in this study (sheep).
Measurements
pHi recovery rate
0.33 pH units (15 min)(-1)
LR3-IGF-1 20 μg l(-1)
Source sentences
Summary
Application of LR3-IGF-1 (20 μg l(-1)) significantly increased the rate of pH(i) recovery to 0.33 ± 0.02 pH units (15 min)(-1).
2 context sentences(design, population, supporting numbers)
Summary · context
A further study was performed with cultured rumen epithelial cells and a fluorescent probe (BCECF) to estimate the rate of pH(i) recovery after acid loading.
Summary · context
The pH(i) of isolated rumen epithelial cells was 6.43 ± 0.15 after butyrate loading and recovered by 0.26 ± 0.02 pH units (15 min)(-1).
Report a problemFND-2222720005

Studies that did not test the peptide alone2

In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking IGF-1 LR3.

05LabNot a test of IGF-1 LR3 aloneEx vivo tissueMechanism
In isolated rumen epithelium of hay-fed sheep, mucosal amiloride inhibited the LR3-IGF-1-induced increase in mucosal-to-serosal and net Na+ flux.
In plain wordsIn lab tests on sheep rumen tissue, the blocker amiloride stopped the sodium flux increase caused by LR3-IGF-1.
Peptide
IGF-1 LR3
Outcome
DecreaseAmiloride block of the LR3-IGF-1 effect on Na+ flux
Study
sheepn: not reported in source
isolated rumen epithelium of hay-fed sheep
Comparator
LR3-IGF-1 without amiloride
Outcome group
Electrolyte balance · Gastrointestinal
Measurements
amiloride concentration
1 mmol l(-1)
mucosal
Source sentences
Summary
The IGF-1-induced increment of J(ms)Na and J(net)Na was inhibited by mucosal amiloride (1 mmol l(-1)).
1 context sentence(design, population, supporting numbers)
Summary · context
This study examined the in vitro effect of IGF-1 on Na(+) transport across the rumen epithelium of hay-fed sheep, in which the IGF-1 concentration in plasma is lower than in concentrate-fed animals.
In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.
Report a problemFND-2222720003
06LabNot a test of IGF-1 LR3 aloneIn vitro (cells)Mechanism
In cultured sheep rumen epithelial cells, amiloride reduced the pH recovery rate in both control and IGF-1-stimulated cells.
In plain wordsIn sheep rumen cells grown in the lab, the blocker amiloride slowed pH recovery in control and IGF-1-treated cells.
Peptide
IGF-1 LR3
Outcome
DecreaseAmiloride block of pH recovery rate
Study
sheepn: not reported in source
cultured rumen epithelial cells
Comparator
Cells without amiloride
Outcome group
Electrolyte balance · Gastrointestinal
Source sentences
Summary
Amiloride administration reduced the recovery rate in both control and IGF-1-stimulated cells.
1 context sentence(design, population, supporting numbers)
Summary · context
A further study was performed with cultured rumen epithelial cells and a fluorescent probe (BCECF) to estimate the rate of pH(i) recovery after acid loading.
In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.
Report a problemFND-2222720006

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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