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Study · In vitro (cell-free) · 2011

13 results

Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA.

Fedoreyeva LI, Kireev II, Khavinson VKh, Vanyushin BF

Biochemistry. Biokhimiia1 Nov 2011Cited by 29 (Europe PMC)

PMID22117547 (opens in a new tab)DOI10.1134/s0006297911110022 (opens in a new tab)PepBankPUB-22117547
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study13

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cell-free)Activity measurement

    In cell-free assays, epithalon, pinealon and bronchogen bound preferentially to deoxyribooligonucleotides containing CNG sequences.

    In plain wordsIn lab tests without cells, epithalon and two other peptides bound mainly to DNA fragments with a certain sequence pattern.

    Peptide
    Epitalon
    Outcome
    Not applicableBinding preference for CNG sequences
    Study

    n: not reported in source

    fluorescence-labeled deoxyribooligonucleotides and DNA
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Judging from corresponding constants of the fluorescence quenching, the epithalon, pinealon, and bronchogen (Ala-Glu-Asp-Leu) bind preferentially with deoxyribooligonucleotides containing CNG sequence (CNG sites are targets for cytosine DNA methylation in eukaryotes).
  2. 02LabIn vitro (cell-free)Activity measurement

    In cell-free assays, epithalon, testagen and pinealon seemed to bind preferentially to CAG-containing sequences.

    In plain wordsIn lab tests without cells, epithalon and two other peptides seemed to bind mainly to DNA fragments containing CAG.

    Peptide
    Epitalon
    Outcome
    Not applicableBinding preference for CAG sequences
    Study

    n: not reported in source

    fluorescence-labeled deoxyribooligonucleotides and DNA
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Epithalon, testagen, and pinealon seem to preferentially bind with CAG- but bronchogen with CTG-containing sequences.
  3. 03LabIn vitro (cell-free)Mechanism

    In cell-free assays, bronchogen preferentially bound deoxyribooligonucleotides containing CNG sequences.

    In plain wordsIn lab tests, bronchogen preferred binding to short DNA molecules containing CNG sequences.

    Peptide
    Bronchogen (AEDL)
    Outcome
    Not applicableCNG sequence binding preference
    Study

    n: not reported in source

    Deoxyribooligonucleotides in fluorescence-quenching assays
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Judging from corresponding constants of the fluorescence quenching, the epithalon, pinealon, and bronchogen (Ala-Glu-Asp-Leu) bind preferentially with deoxyribooligonucleotides containing CNG sequence (CNG sites are targets for cytosine DNA methylation in eukaryotes).
  4. 04LabIn vitro (cell-free)Mechanism

    In cell-free assays, bronchogen appeared to bind preferentially to CTG-containing sequences.

    In plain wordsIn lab tests, bronchogen seemed to prefer DNA sequences containing CTG.

    Peptide
    Bronchogen (AEDL)
    Outcome
    Not applicableCTG sequence binding preference
    Study

    n: not reported in source

    Deoxyribooligonucleotides in fluorescence-quenching assays
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Epithalon, testagen, and pinealon seem to preferentially bind with CAG- but bronchogen with CTG-containing sequences.
  5. 05LabIn vitro (cell-free)Activity measurement

    In cell-free assays, Testagen seemed to bind preferentially to CAG-containing sequences.

    In plain wordsIn lab tests, Testagen seemed to bind preferentially to sequences containing CAG.

    Peptide
    Testagen (KEDG)
    Outcome
    Not applicablePreferential binding to CAG-containing sequences
    Study

    n: not reported in source

    Deoxyribooligonucleotides
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Epithalon, testagen, and pinealon seem to preferentially bind with CAG- but bronchogen with CTG-containing sequences.

Studies that did not test the peptide alone8

In this study, the peptide was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking the peptide.

  1. 06LabNot a test of Pinealon aloneIn vitro (cells)Mechanism

    In HeLa cells, fluorescence appeared in cytoplasm, nucleus, and nucleolus after incubation with FITC-labeled pinealon.

    In plain wordsIn cells, labeled pinealon produced fluorescence in the cytoplasm, nucleus, and nucleolus.

    Peptide
    Pinealon
    Outcome
    Not applicableIntracellular localization
    Study

    humann: not reported in source

    HeLa cells
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Marked fluorescence in cytoplasm, nucleus, and nucleolus was observed in HeLa cells after incubation with each of several fluorescein isothiocyanate-labeled peptides (epithalon, Ala-Glu-Asp-Gly; pinealon, Glu-Asp-Arg; testagen, Lys-Glu-Asp-Gly).

    In this study, Pinealon was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Pinealon.

  2. 07LabNot a test of Epitalon aloneIn vitro (cell-free)Activity measurement

    In cell-free assays, the Stern-Volmer constants of fluorescence quenching of labeled oligonucleotides differed between the short peptides, depending on peptide primary structure.

    In plain wordsIn lab tests without cells, short peptides, including epithalon, affected labeled DNA fragments differently depending on their structure.

    Peptide
    Epitalon
    Outcome
    Not applicableFluorescence quenching of labeled oligonucleotides, by peptide structure
    Study

    n: not reported in source

    fluorescence-labeled deoxyribooligonucleotides and DNA
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    The Stern-Volmer constants characterizing the degree of fluorescence quenching of various single- and double-stranded fluorescence-labeled deoxyribooligonucleotides with short peptides used were different depending on the peptide primary structures.

    In this study, Epitalon was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Epitalon.

  3. 08LabNot a test of Pinealon aloneIn vitro (cell-free)Mechanism

    In cell-free tests, intact short peptides including pinealon affected fluorescence of labeled deoxyribooligonucleotides and DNA complexes differently.

    In plain wordsIn lab tests, intact short peptides including pinealon changed fluorescence of labeled DNA structures in different ways.

    Peptide
    Pinealon
    Outcome
    MixedNucleic acid fluorescence modulation
    Study

    n: not reported in source

    Labeled deoxyribooligonucleotides and DNA-ethidium bromide complexes
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    It was established that various initial (intact) peptides differently affect the fluorescence of the 5,6-carboxyfluorescein-labeled deoxyribooligonucleotides and DNA-ethidium bromide complexes.
    1 context sentence
    Summary · context
    Marked fluorescence in cytoplasm, nucleus, and nucleolus was observed in HeLa cells after incubation with each of several fluorescein isothiocyanate-labeled peptides (epithalon, Ala-Glu-Asp-Gly; pinealon, Glu-Asp-Arg; testagen, Lys-Glu-Asp-Gly).

    In this study, Pinealon was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Pinealon.

  4. 09LabNot a test of Pinealon aloneIn vitro (cell-free)Mechanism

    In cell-free tests, short-peptide fluorescence-quenching constants differed with peptide primary structure.

    In plain wordsIn lab tests, fluorescence quenching by short peptides differed with the peptide sequence.

    Peptide
    Pinealon
    Outcome
    Not applicableSequence-dependent fluorescence quenching
    Study

    n: not reported in source

    Fluorescence-labeled single- and double-stranded deoxyribooligonucleotides
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    The Stern-Volmer constants characterizing the degree of fluorescence quenching of various single- and double-stranded fluorescence-labeled deoxyribooligonucleotides with short peptides used were different depending on the peptide primary structures.
    1 context sentence
    Summary · context
    Marked fluorescence in cytoplasm, nucleus, and nucleolus was observed in HeLa cells after incubation with each of several fluorescein isothiocyanate-labeled peptides (epithalon, Ala-Glu-Asp-Gly; pinealon, Glu-Asp-Arg; testagen, Lys-Glu-Asp-Gly).

    In this study, Pinealon was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Pinealon.

  5. 10LabNot a test of Pinealon aloneIn vitro (cell-free)Mechanism

    In cell-free tests, short peptides including pinealon discriminated between different nucleotide sequences.

    In plain wordsIn lab tests, short peptides including pinealon distinguished different DNA sequences.

    Peptide
    Pinealon
    Outcome
    Not applicableRecognition of nucleotide sequences
    Study

    n: not reported in source

    Nucleic acid structures
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    On binding to them, the peptides discriminate between different nucleotide sequences and recognize even their cytosine methylation status.
    1 context sentence
    Summary · context
    Marked fluorescence in cytoplasm, nucleus, and nucleolus was observed in HeLa cells after incubation with each of several fluorescein isothiocyanate-labeled peptides (epithalon, Ala-Glu-Asp-Gly; pinealon, Glu-Asp-Arg; testagen, Lys-Glu-Asp-Gly).

    In this study, Pinealon was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Pinealon.

  6. 11LabNot a test of Pinealon aloneIn vitro (cell-free)Mechanism

    In cell-free tests, pinealon preferentially bound deoxyribooligonucleotides containing CNG sequences, according to fluorescence-quenching constants.

    In plain wordsIn lab tests, pinealon preferentially bound short DNA sequences containing CNG.

    Peptide
    Pinealon
    Outcome
    Not applicablePreferential CNG sequence binding
    Study

    n: not reported in source

    Deoxyribooligonucleotides
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Judging from corresponding constants of the fluorescence quenching, the epithalon, pinealon, and bronchogen (Ala-Glu-Asp-Leu) bind preferentially with deoxyribooligonucleotides containing CNG sequence (CNG sites are targets for cytosine DNA methylation in eukaryotes).

    In this study, Pinealon was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Pinealon.

  7. 12LabNot a test of Pinealon aloneIn vitro (cell-free)Mechanism

    In cell-free tests, pinealon appeared to preferentially bind CAG-containing sequences.

    In plain wordsIn lab tests, pinealon appeared to preferentially bind DNA sequences containing CAG.

    Peptide
    Pinealon
    Outcome
    Not applicablePreferential CAG sequence binding
    Study

    n: not reported in source

    Deoxyribooligonucleotides
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    Epithalon, testagen, and pinealon seem to preferentially bind with CAG- but bronchogen with CTG-containing sequences.

    In this study, Pinealon was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Pinealon.

  8. 13LabNot a test of Pinealon aloneIn vitro (cell-free)Mechanism

    In cell-free tests, short peptides including pinealon recognized cytosine methylation status in nucleic acid structures.

    In plain wordsIn lab tests, short peptides including pinealon recognized the cytosine methylation status of DNA structures.

    Peptide
    Pinealon
    Outcome
    Not applicableRecognition of cytosine methylation status
    Study

    n: not reported in source

    Nucleic acid structures
    Outcome group
    Receptor activity and mechanism · Pharmacology

    Source sentences

    Summary
    On binding to them, the peptides discriminate between different nucleotide sequences and recognize even their cytosine methylation status.
    1 context sentence
    Summary · context
    Marked fluorescence in cytoplasm, nucleus, and nucleolus was observed in HeLa cells after incubation with each of several fluorescein isothiocyanate-labeled peptides (epithalon, Ala-Glu-Asp-Gly; pinealon, Glu-Asp-Arg; testagen, Lys-Glu-Asp-Gly).

    In this study, Pinealon was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Pinealon.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Versions 10 to 17Current

    28 Oct 2026, 02:02 UTC

    The same notice was added 8 times, first on 21 Oct 2026, 17:05 UTC. The status did not change between them.

    Study results updated

  2. Versions 7 to 9

    21 Oct 2026, 16:35 UTC

    The same notice was added 3 times, first on 21 Oct 2026, 16:35 UTC. The status did not change between them.

    Study data updated · source update from Europe PMC

  3. Versions 4 to 6

    21 Oct 2026, 15:58 UTC

    The same notice was added 3 times, first on 14 Oct 2026, 05:02 UTC. The status did not change between them.

    Study results updated

  4. Version 3

    14 Oct 2026, 01:46 UTC

    Study data updated · source update from Europe PMC

  5. Version 2

    10 Oct 2026, 08:19 UTC

    Study results updated

  6. Version 1

    9 Oct 2026, 02:47 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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