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Study · In vitro (cells) · 2012

4 results

Expression and purification of two alternative peptides for mechano-growth factor in Escherichia coli.

Song L, Zhu WS, Dong JL, Li ZY, Liu XH

Biotechnology letters4 Oct 2011Cited by 2 (Europe PMC)

PMID21968480 (opens in a new tab)DOI10.1007/s10529-011-0762-x (opens in a new tab)PepBankPUB-21968480
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In C2C12 cells, recombinant MGFc(40) and MGFc(24) stimulated cell proliferation.

    In plain wordsIn cells, two recombinant MGF peptides made muscle cells (C2C12) multiply more.

    Peptide
    MGF / PEG-MGF
    Outcome
    IncreaseC2C12 cell proliferation
    Study

    n: not reported in source

    C2C12 cells
    Outcome group
    Muscle injury and repair · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    MGFc(40) and MGFc(24), which are involved in proliferation and differentiation, stimulated cell proliferation and inhibited cell differentiation of C2C12 cells.
  2. 02LabIn vitro (cells)Activity measurement

    In C2C12 cells, recombinant MGFc(40) and MGFc(24) inhibited cell differentiation.

    In plain wordsIn cells, two recombinant MGF peptides slowed the maturing of muscle cells (C2C12).

    Peptide
    MGF / PEG-MGF
    Outcome
    DecreaseC2C12 cell differentiation
    Study

    n: not reported in source

    C2C12 cells
    Outcome group
    Muscle injury and repair · Musculoskeletal
    Studied dose
    Dose not stated

    Source sentences

    Summary
    MGFc(40) and MGFc(24), which are involved in proliferation and differentiation, stimulated cell proliferation and inhibited cell differentiation of C2C12 cells.

Studies that did not test the peptide alone2

In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking MGF / PEG-MGF.

  1. 03LabNot a test of MGF / PEG-MGF aloneIn vitro (cell-free)Other

    Recombinant MGFc(40) protein was expressed in Escherichia coli and purified by affinity chromatography at 4 mg per litre.

    In plain wordsIn lab tests, bacteria made a recombinant MGF peptide (MGFc(40)) that was purified, at 4 mg per litre.

    Peptide
    MGF / PEG-MGF
    Outcome
    Not applicableYield of recombinant MGFc(40) protein
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology

    Measurements

    MGFc(40) yield
    4 mg l(-1)
    E. coli expression

    Source sentences

    Summary
    Recombinant MGFc(40) protein (4 mg l(-1)) was expressed and purified by affinity chromatography using His60 Ni Superflow.

    In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.

  2. 04LabNot a test of MGF / PEG-MGF aloneIn vitro (cell-free)Other

    After enzymatic cleavage of the GST-tail, 1 mg of MGFc(24) protein per litre was obtained.

    In plain wordsIn lab tests, bacteria made a recombinant MGF peptide (MGFc(24)) that was purified, at 1 mg per litre.

    Peptide
    MGF / PEG-MGF
    Outcome
    Not applicableYield of recombinant MGFc(24) protein
    Study

    n: not reported in source

    Outcome group
    Receptor activity and mechanism · Pharmacology

    Measurements

    MGFc(24) yield
    1 mg l(-1)
    after GST-tail cleavage

    Source sentences

    Summary
    After enzymatic cleavage of the GST-tail, 1 mg MGFc(24) protein l(-1) was obtained.

    In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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