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Study · In vitro (cells) · mouse · 2011

12 results

Functional interaction of bone morphogenetic protein and growth hormone releasing peptide in adrenocorticotropin regulation by corticotrope cells.

Tsukamoto N, Otsuka F, Miyoshi T, Inagaki K, Nakamura E, Terasaka T, Takeda M, Ogura T, Iwasaki Y, Makino H

Molecular and cellular endocrinology1 Jul 2011Cited by 15 (Europe PMC)

PMID21742013 (opens in a new tab)DOI10.1016/j.mce.2011.06.016 (opens in a new tab)PepBankPUB-21742013
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study12

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Mechanism

    In mouse AtT20 corticotrope cells, GHRP‑2 increased ACTH secretion, less potently than CRH.

    In plain wordsIn lab tests on mouse pituitary cells, GHRP‑2 raised ACTH release, but less than CRH did.

    Peptide
    GHRP-2
    Outcome
    IncreaseACTH secretion
    Study

    mousen: not reported in source

    AtT20 corticotrope cells
    Outcome group
    Stress hormones · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    GHRP-2 increased ACTH and cAMP secretion by AtT20 cells; however, its effects were less potent than the effects of CRH.
    1 context sentence
    Summary · context
    Mechanisms by which GHRP stimulates ACTH release in corticotrope cells were investigated using mouse corticotrope AtT20 cells by focusing on the biological activity of BMP-4.
  2. 02LabIn vitro (cells)Mechanism

    In mouse AtT20 corticotrope cells, GHRP‑2 increased cAMP secretion, less potently than CRH.

    In plain wordsIn lab tests on mouse pituitary cells, GHRP‑2 raised cAMP release, but less than CRH did.

    Peptide
    GHRP-2
    Outcome
    IncreasecAMP secretion
    Study

    mousen: not reported in source

    AtT20 corticotrope cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    GHRP-2 increased ACTH and cAMP secretion by AtT20 cells; however, its effects were less potent than the effects of CRH.
    1 context sentence
    Summary · context
    Mechanisms by which GHRP stimulates ACTH release in corticotrope cells were investigated using mouse corticotrope AtT20 cells by focusing on the biological activity of BMP-4.
  3. 03LabIn vitro (cells)Mechanism

    In mouse AtT20 cells, GHRP‑2, unlike CRH, did not activate ERK1/2, p38, SAPK/JNK or Akt phosphorylation.

    In plain wordsIn lab tests on mouse pituitary cells, GHRP‑2 did not switch on these kinase pathways, while CRH did.

    Peptide
    GHRP-2
    Outcome
    No significant changeERK1/2, p38, SAPK/JNK and Akt phosphorylation
    Study

    mousen: not reported in source

    AtT20 corticotrope cells
    Comparator
    CRH
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Stimulation with CRH, but not GHRP-2, activated ERK1/2, p38, SAPK/JNK and Akt phosphorylation
    1 context sentence
    Summary · context
    Mechanisms by which GHRP stimulates ACTH release in corticotrope cells were investigated using mouse corticotrope AtT20 cells by focusing on the biological activity of BMP-4.
  4. 04LabIn vitro (cells)Mechanism

    In mouse AtT20 cells, GHRP‑2 upregulated BMP receptor signaling.

    In plain wordsIn lab tests on mouse pituitary cells, GHRP‑2 turned up BMP receptor signaling.

    Peptide
    GHRP-2
    Outcome
    IncreaseBMP receptor signaling
    Study

    mousen: not reported in source

    AtT20 corticotrope cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, CRH and GHRP-2 upregulated BMP receptor signaling, while BMP-4, CRH and GHRP-2 had no significant effect on the expression level of GHSR.
    1 context sentence
    Summary · context
    Mechanisms by which GHRP stimulates ACTH release in corticotrope cells were investigated using mouse corticotrope AtT20 cells by focusing on the biological activity of BMP-4.
  5. 05LabIn vitro (cells)Mechanism

    In mouse AtT20 cells, GHRP‑2 had no significant effect on the expression level of GHSR.

    In plain wordsIn lab tests on mouse pituitary cells, GHRP‑2 did not change the level of its receptor GHSR.

    Peptide
    GHRP-2
    Outcome
    No significant changeGHSR expression
    Study

    mousen: not reported in source

    AtT20 corticotrope cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Moreover, CRH and GHRP-2 upregulated BMP receptor signaling, while BMP-4, CRH and GHRP-2 had no significant effect on the expression level of GHSR.
    1 context sentence
    Summary · context
    Mechanisms by which GHRP stimulates ACTH release in corticotrope cells were investigated using mouse corticotrope AtT20 cells by focusing on the biological activity of BMP-4.
  6. 06LabIn vitro (cells)Mechanism

    In mouse AtT20 cells, GHRP‑2 suppressed the expression of Smad7, an inhibitor of the BMP-Smad1/5/8 pathway.

    In plain wordsIn lab tests on mouse pituitary cells, GHRP‑2 lowered Smad7, a brake on the BMP pathway.

    Peptide
    GHRP-2
    Outcome
    DecreaseSmad7 expression
    Study

    mousen: not reported in source

    AtT20 corticotrope cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In addition, GHRP-2 suppressed the expression of Smad7, which is an inhibitor of the BMP-Smad1/5/8 pathway.
    1 context sentence
    Summary · context
    Mechanisms by which GHRP stimulates ACTH release in corticotrope cells were investigated using mouse corticotrope AtT20 cells by focusing on the biological activity of BMP-4.

Studies that did not test the peptide alone6

In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-2.

  1. 07LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism

    In mouse AtT20 cells, BMP-4 suppressed GHRP‑2-induced ACTH production, less efficaciously than CRH-induced production.

    In plain wordsIn lab tests on mouse pituitary cells, BMP-4 reduced ACTH made after GHRP‑2, but less than after CRH.

    Peptide
    GHRP-2
    Outcome
    DecreaseGHRP-2-induced ACTH production after BMP-4
    Study

    mousen: not reported in source

    AtT20 corticotrope cells
    Outcome group
    Stress hormones · Endocrine and hormones

    Source sentences

    Summary
    Of note, BMP-4 suppressed ACTH production and POMC-promoter activity induced by CRH more efficaciously than that induced by GHRP-2.
    1 context sentence
    Summary · context
    Mechanisms by which GHRP stimulates ACTH release in corticotrope cells were investigated using mouse corticotrope AtT20 cells by focusing on the biological activity of BMP-4.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

  2. 08LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism

    In mouse AtT20 cells, BMP-4 suppressed GHRP‑2-induced POMC-promoter activity, less efficaciously than CRH-induced activity.

    In plain wordsIn lab tests on mouse pituitary cells, BMP-4 reduced POMC promoter activity after GHRP‑2, but less than after CRH.

    Peptide
    GHRP-2
    Outcome
    DecreaseGHRP-2-induced POMC-promoter activity after BMP-4
    Study

    mousen: not reported in source

    AtT20 corticotrope cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Source sentences

    Summary
    Of note, BMP-4 suppressed ACTH production and POMC-promoter activity induced by CRH more efficaciously than that induced by GHRP-2.
    1 context sentence
    Summary · context
    Mechanisms by which GHRP stimulates ACTH release in corticotrope cells were investigated using mouse corticotrope AtT20 cells by focusing on the biological activity of BMP-4.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

  3. 09LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism

    In mouse AtT20 cells, BMP-4 had no significant effect on GHRP‑2-induced cAMP synthesis.

    In plain wordsIn lab tests on mouse pituitary cells, BMP-4 did not change cAMP made after GHRP‑2.

    Peptide
    GHRP-2
    Outcome
    No significant changeGHRP-2-induced cAMP synthesis after BMP-4
    Study

    mousen: not reported in source

    AtT20 corticotrope cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Source sentences

    Summary
    BMP-4 had no significant effect on cAMP synthesis induced by CRH or GHRP-2.
    1 context sentence
    Summary · context
    Mechanisms by which GHRP stimulates ACTH release in corticotrope cells were investigated using mouse corticotrope AtT20 cells by focusing on the biological activity of BMP-4.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

  4. 10LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism

    In mouse AtT20 cells, inhibitors of the ERK, p38 and Akt pathways did not affect GHRP‑2-induced ACTH secretion.

    In plain wordsIn lab tests on mouse pituitary cells, blocking ERK, p38 or Akt did not change ACTH release caused by GHRP‑2.

    Peptide
    GHRP-2
    Outcome
    No significant changeGHRP-2-induced ACTH secretion after ERK, p38 and Akt inhibitors
    Study

    mousen: not reported in source

    AtT20 corticotrope cells
    Outcome group
    Stress hormones · Endocrine and hormones

    Source sentences

    Summary
    GHRP-2-induced ACTH secretion was not affected by inhibitors of ERK, p38 and Akt pathways, which effectively suppressed CRH-induced ACTH release.
    1 context sentence
    Summary · context
    Mechanisms by which GHRP stimulates ACTH release in corticotrope cells were investigated using mouse corticotrope AtT20 cells by focusing on the biological activity of BMP-4.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

  5. 11LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism

    In mouse AtT20 cells, blockage of the cAMP-PKA pathway reversed GHRP‑2-induced ACTH secretion.

    In plain wordsIn lab tests on mouse pituitary cells, blocking the cAMP-PKA pathway reversed ACTH release caused by GHRP‑2.

    Peptide
    GHRP-2
    Outcome
    DecreaseGHRP-2-induced ACTH secretion after cAMP-PKA blockade
    Study

    mousen: not reported in source

    AtT20 corticotrope cells
    Outcome group
    Stress hormones · Endocrine and hormones

    Source sentences

    Summary
    Blockage of the cAMP-PKA pathway reversed CRH- as well as GHRP-2-induced ACTH secretion.
    1 context sentence
    Summary · context
    Mechanisms by which GHRP stimulates ACTH release in corticotrope cells were investigated using mouse corticotrope AtT20 cells by focusing on the biological activity of BMP-4.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

  6. 12LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism

    In mouse AtT20 cells, inhibition of ERK and p38 did not reduce GHRP‑2-induced cAMP synthesis, but did reduce CRH-induced synthesis.

    In plain wordsIn lab tests on mouse pituitary cells, blocking ERK and p38 did not lower cAMP made after GHRP‑2.

    Peptide
    GHRP-2
    Outcome
    No significant changeGHRP-2-induced cAMP synthesis after ERK and p38 inhibition
    Study

    mousen: not reported in source

    AtT20 corticotrope cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones

    Source sentences

    Summary
    Furthermore, the inhibition of ERK and p38 significantly reduced cAMP synthesis induced by CRH but not by GHRP-2.
    1 context sentence
    Summary · context
    Mechanisms by which GHRP stimulates ACTH release in corticotrope cells were investigated using mouse corticotrope AtT20 cells by focusing on the biological activity of BMP-4.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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