Expression, purification and characterization of a novel soluble human thymosin alpha1 concatemer exhibited a stronger stimulation on mice lymphocytes proliferation and higher anti-tumor activity.

Li W, Song L, Wu S, Xue X, Zhang L, He L, Han W, Wang Q, Ling R, Zhang W, Yan Z, Zhang Y

International journal of biological sciences19 May 2011Cited by 5 (Europe PMC)

PMID21647330PMCIDPMC3107470DOI10.7150/ijbs.7.618PepBankPUB-21647330

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone4

In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin alpha-1.

01AnimalNot a test of Thymosin alpha-1 aloneAnimal study (in vivo)Efficacy outcome
In B16 tumor-bearing mice, the thymosin alpha‑1 concatemer Tα1② significantly inhibited tumor growth.
In plain wordsIn mice with B16 tumors, the thymosin alpha‑1 concatemer slowed tumor growth.
Peptide
Thymosin alpha-1
Outcome
DecreaseTumor growth in B16 tumor-bearing mice
Study
mousen: not reported in source
B16 tumor-bearing mice
Outcome group
Tumor growth · Cancer
Source sentences
Summary
In vivo, Tα1② significantly inhibited the tumor growth in B16 tumor-bearing mice.
1 context sentence(design, population, supporting numbers)
Summary · context
We expressed the recombinant 2×Tα1 concatemer protein (Tα1② protein) in Escherichia coli.
In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.
Report a problemFND-2164733004
02LabNot a test of Thymosin alpha-1 aloneIn vitro (cells)Efficacy outcome
In vitro, the thymosin alpha‑1 concatemer Tα1② stimulated the proliferation of mouse splenic lymphocytes.
In plain wordsIn lab tests on mouse cells, the thymosin alpha‑1 concatemer increased immune cell growth.
Peptide
Thymosin alpha-1
Outcome
IncreaseProliferation of mouse splenic lymphocytes
Study
mousen: not reported in source
Mouse splenic lymphocytes
Outcome group
Immune response · Immune and inflammation
Source sentences
Summary
In vitro, Tα1② could stimulate the proliferation of mouse splenic lymphocyte, and increase the apoptosis of tumor cell lines.
1 context sentence(design, population, supporting numbers)
Summary · context
We expressed the recombinant 2×Tα1 concatemer protein (Tα1② protein) in Escherichia coli.
In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.
Report a problemFND-2164733002
03LabNot a test of Thymosin alpha-1 aloneIn vitro (cells)Efficacy outcome
In vitro, the thymosin alpha‑1 concatemer Tα1② increased apoptosis of tumor cell lines.
In plain wordsIn lab tests on tumor cells, the thymosin alpha‑1 concatemer increased cell death.
Peptide
Thymosin alpha-1
Outcome
IncreaseApoptosis of tumor cell lines
Study
n: not reported in source
Tumor cell lines
Outcome group
Tumor growth · Cancer
Source sentences
Summary
In vitro, Tα1② could stimulate the proliferation of mouse splenic lymphocyte, and increase the apoptosis of tumor cell lines.
1 context sentence(design, population, supporting numbers)
Summary · context
We expressed the recombinant 2×Tα1 concatemer protein (Tα1② protein) in Escherichia coli.
In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.
Report a problemFND-2164733003
04LabNot a test of Thymosin alpha-1 aloneIn vitro (cell-free)Other
The recombinant thymosin alpha‑1 concatemer Tα1② had a purity above 95% by HPLC.
In plain wordsIn lab production, the thymosin alpha‑1 concatemer was more than 95% pure.
Peptide
Thymosin alpha-1
Outcome
Not applicablePurity of Tα1② concatemer
Study
n: not reported in source
Recombinant expression in Escherichia coli
Outcome group
Synthesis and manufacturing · Pharmacology
Measurements
Purity
> 95%
Tα1②
Source sentences
Summary
The purity of Tα1② was higher than 95% as assessed by HPLC analysis.
1 context sentence(design, population, supporting numbers)
Summary · context
We expressed the recombinant 2×Tα1 concatemer protein (Tα1② protein) in Escherichia coli.
In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.
Report a problemFND-2164733001

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 3Current
10 Oct 2026, 08:55 UTC
Study results updated
Version 2
9 Oct 2026, 03:03 UTC
Study data updated · source update from Europe PMC
Version 1
9 Oct 2026, 03:02 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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