The effects of ghrelin/GHSs on AVP mRNA expression and release in cultured hypothalamic cells in rats.

Nemoto T, Sugihara H, Mano A, Kano T, Shibasaki T

Peptides14 Apr 2011Cited by 10 (Europe PMC)

PMID21514337DOI10.1016/j.peptides.2011.04.007PepBankPUB-21514337

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

3 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In cultured rat hypothalamic cells, KP-102 (GHRP‑2) incubation for 4 h and 8 h increased AVP mRNA expression.
In plain wordsIn lab tests on rat brain cells, GHRP‑2 raised the amount of vasopressin messenger RNA.
Peptide
GHRP-2
Outcome
IncreaseAVP mRNA expression
Study
ratn: not reported in source
Monolayer-cultured hypothalamic cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated · 4 h and 8 hAs given in this study (rat).
Source sentences
Summary
Incubation of cells with KP-102 for 4h and 8h and with ghrelin for 4h significantly increased AVP mRNA expression and release without changing CRF mRNA expression.
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify the mechanisms of the action of ghrelin/GHSs on ACTH secretion, we analyzed the effects of KP-102 and ghrelin on the mRNA expression and release of corticotropin releasing factor (CRF) and arginine vasopressin (AVP), ACTH secretagogues, in monolayer-cultured hypothalamic cells of rats.
Report a problemFND-2151433701
02LabIn vitro (cells)Mechanism
In cultured rat hypothalamic cells, KP-102 (GHRP‑2) incubation for 4 h and 8 h increased AVP release.
In plain wordsIn lab tests on rat brain cells, GHRP‑2 raised the release of vasopressin.
Peptide
GHRP-2
Outcome
IncreaseAVP release
Study
ratn: not reported in source
Monolayer-cultured hypothalamic cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated · 4 h and 8 hAs given in this study (rat).
Source sentences
Summary
Incubation of cells with KP-102 for 4h and 8h and with ghrelin for 4h significantly increased AVP mRNA expression and release without changing CRF mRNA expression.
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify the mechanisms of the action of ghrelin/GHSs on ACTH secretion, we analyzed the effects of KP-102 and ghrelin on the mRNA expression and release of corticotropin releasing factor (CRF) and arginine vasopressin (AVP), ACTH secretagogues, in monolayer-cultured hypothalamic cells of rats.
Report a problemFND-2151433702
03LabIn vitro (cells)Mechanism
In cultured rat hypothalamic cells, KP-102 (GHRP‑2) did not change CRF mRNA expression.
In plain wordsIn lab tests on rat brain cells, GHRP‑2 did not change the amount of CRF messenger RNA.
Peptide
GHRP-2
Outcome
No significant changeCRF mRNA expression
Study
ratn: not reported in source
Monolayer-cultured hypothalamic cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated · 4 h and 8 hAs given in this study (rat).
Source sentences
Summary
Incubation of cells with KP-102 for 4h and 8h and with ghrelin for 4h significantly increased AVP mRNA expression and release without changing CRF mRNA expression
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify the mechanisms of the action of ghrelin/GHSs on ACTH secretion, we analyzed the effects of KP-102 and ghrelin on the mRNA expression and release of corticotropin releasing factor (CRF) and arginine vasopressin (AVP), ACTH secretagogues, in monolayer-cultured hypothalamic cells of rats.
Report a problemFND-2151433703

Studies that did not test the peptide alone4

In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-2.

04LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In cultured rat hypothalamic cells, siRNA suppression of GHS-R expression blocked the KP-102-induced increase in AVP mRNA expression.
In plain wordsIn lab tests on rat brain cells, switching off the GHS receptor stopped GHRP‑2 from raising vasopressin messenger RNA.
Peptide
GHRP-2
Outcome
DecreaseKP-102-induced AVP mRNA expression after GHS-R siRNA
Study
ratn: not reported in source
Monolayer-cultured hypothalamic cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Suppression of GHS-R expression by siRNA blocked ghrelin- and KP-102-induced AVP mRNA expression and release.
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify the mechanisms of the action of ghrelin/GHSs on ACTH secretion, we analyzed the effects of KP-102 and ghrelin on the mRNA expression and release of corticotropin releasing factor (CRF) and arginine vasopressin (AVP), ACTH secretagogues, in monolayer-cultured hypothalamic cells of rats.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-2151433704
05LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In cultured rat hypothalamic cells, siRNA suppression of GHS-R expression blocked the KP-102-induced increase in AVP release.
In plain wordsIn lab tests on rat brain cells, switching off the GHS receptor stopped GHRP‑2 from raising vasopressin release.
Peptide
GHRP-2
Outcome
DecreaseKP-102-induced AVP release after GHS-R siRNA
Study
ratn: not reported in source
Monolayer-cultured hypothalamic cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Suppression of GHS-R expression by siRNA blocked ghrelin- and KP-102-induced AVP mRNA expression and release.
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify the mechanisms of the action of ghrelin/GHSs on ACTH secretion, we analyzed the effects of KP-102 and ghrelin on the mRNA expression and release of corticotropin releasing factor (CRF) and arginine vasopressin (AVP), ACTH secretagogues, in monolayer-cultured hypothalamic cells of rats.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-2151433705
06LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In cultured rat hypothalamic cells, anti-NPY IgG blocked the KP-102-induced increase in AVP mRNA expression.
In plain wordsIn lab tests on rat brain cells, an NPY antibody stopped GHRP‑2 from raising vasopressin messenger RNA.
Peptide
GHRP-2
Outcome
DecreaseKP-102-induced AVP mRNA expression after anti-NPY IgG
Study
ratn: not reported in source
Monolayer-cultured hypothalamic cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Furthermore, treatment of cells with anti-NPY IgG blocked KP-102-induced AVP mRNA expression and release.
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify the mechanisms of the action of ghrelin/GHSs on ACTH secretion, we analyzed the effects of KP-102 and ghrelin on the mRNA expression and release of corticotropin releasing factor (CRF) and arginine vasopressin (AVP), ACTH secretagogues, in monolayer-cultured hypothalamic cells of rats.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-2151433706
07LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In cultured rat hypothalamic cells, anti-NPY IgG blocked the KP-102-induced increase in AVP release.
In plain wordsIn lab tests on rat brain cells, an NPY antibody stopped GHRP‑2 from raising vasopressin release.
Peptide
GHRP-2
Outcome
DecreaseKP-102-induced AVP release after anti-NPY IgG
Study
ratn: not reported in source
Monolayer-cultured hypothalamic cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Furthermore, treatment of cells with anti-NPY IgG blocked KP-102-induced AVP mRNA expression and release.
1 context sentence(design, population, supporting numbers)
Summary · context
To clarify the mechanisms of the action of ghrelin/GHSs on ACTH secretion, we analyzed the effects of KP-102 and ghrelin on the mRNA expression and release of corticotropin releasing factor (CRF) and arginine vasopressin (AVP), ACTH secretagogues, in monolayer-cultured hypothalamic cells of rats.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-2151433707

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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