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Study · Not a test of the peptide alone · 2011

4 results

Separation and purification of Escherichia coli-expressed human thymosin-α1 using affinity chromatography and high-performance liquid chromatography.

Zhang HY, Chen PF, Xu JM, Dai QM, Xu F, Han QW, Wang JJ, Jin HY

Protein expression and purification21 Jan 2011Cited by 3 (Europe PMC)

PMID21256964 (opens in a new tab)DOI10.1016/j.pep.2011.01.004 (opens in a new tab)PepBankPUB-21256964
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone4

In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin alpha-1.

  1. 01LabNot a test of Thymosin alpha-1 aloneIn vitro (cell-free)Other

    The soluble hexahistidine-tagged hTα1 fusion protein made up about 50-60% of total protein in the engineered E. coli strain.

    In plain wordsIn lab production, the thymosin fusion protein made up about half or more of the cell protein.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableShare of recombinant protein in total E. coli protein
    Study

    n: not reported in source

    Recombinant expression in Escherichia coli
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    Share of total protein
    50 to 60%
    hTα1 fusion protein

    Source sentences

    Summary
    The recombinant protein accounted for approximately 50-60% of the total protein.
    1 context sentence
    Summary · context
    In this study, a human thymosin-α1 (hTα1) fusion protein was overexpressed in Escherichia coli (E. coli).

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

  2. 02LabNot a test of Thymosin alpha-1 aloneIn vitro (cell-free)Other

    Purified recombinant hTα1 had a purity above 99% by electrophoresis and HPLC.

    In plain wordsIn lab production, the purified thymosin alpha‑1 was more than 99% pure.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicablePurity of purified recombinant hTα1
    Study

    n: not reported in source

    Recombinant expression in Escherichia coli
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    Purity
    > 99%
    recombinant hTα1

    Source sentences

    Summary
    Purified recombinant hTα1 of high homogeneity was characterized and found to be of high purity (over 99%), as determined by high-voltage electrophoresis and high-performance liquid chromatography analysis.
    1 context sentence
    Summary · context
    In this study, a human thymosin-α1 (hTα1) fusion protein was overexpressed in Escherichia coli (E. coli).

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

  3. 03LabNot a test of Thymosin alpha-1 aloneIn vitro (cell-free)Other

    Isoelectric focusing indicated a pI of approximately 4.0 for recombinant hTα1.

    In plain wordsIn lab tests, thymosin alpha‑1 had an isoelectric point of about 4.0.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableIsoelectric point of hTα1
    Study

    n: not reported in source

    Recombinant expression in Escherichia coli
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    pI
    ≈ 4
    hTα1

    Source sentences

    Summary
    Isoelectric focusing analysis indicated a pI of approximately 4.0
    1 context sentence
    Summary · context
    In this study, a human thymosin-α1 (hTα1) fusion protein was overexpressed in Escherichia coli (E. coli).

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

  4. 04LabNot a test of Thymosin alpha-1 aloneIn vitro (cell-free)Other

    Full wavelength screening showed an optimal absorbance wavelength of around 214 nm for hTα1.

    In plain wordsIn lab tests, thymosin alpha‑1 absorbed light best at about 214 nm.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableOptimal absorbance wavelength of hTα1
    Study

    n: not reported in source

    Recombinant expression in Escherichia coli
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    Optimal absorbance wavelength
    ≈ 214 nm
    hTα1

    Source sentences

    Summary
    full wavelength screening showed an optimal absorbance wavelength at around 214nm.
    1 context sentence
    Summary · context
    In this study, a human thymosin-α1 (hTα1) fusion protein was overexpressed in Escherichia coli (E. coli).

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    10 Oct 2026, 08:55 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 03:02 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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