Insulinlike growth factor-1Ec (MGF) expression in eutopic and ectopic endometrium: characterization of the MGF E-peptide actions in vitro.

Milingos DS, Philippou A, Armakolas A, Papageorgiou E, Sourla A, Protopapas A, Liapi A, Antsaklis A, Mastrominas M, Koutsilieris M

Molecular medicine (Cambridge, Mass.)14 Sept 2010Cited by 33 (Europe PMC)

PMID20844834PMCIDPMC3022988DOI10.2119/molmed.2010.00043PepBankPUB-20844834

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

2 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
Synthetic MGF E-peptide, which comprised the last 24 amino acids of MGF, stimulated the growth of KLE cells.
In plain wordsIn cells, a synthetic MGF E-peptide made KLE cells grow more.
Peptide
MGF / PEG-MGF
Outcome
IncreaseKLE cell growth with synthetic MGF E-peptide
Study
n: not reported in source
KLE cells
Outcome group
Tumor growth · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Furthermore, synthetic MGF E-peptide, which comprised the last 24 amino acids of the MGF, stimulated the growth of the KLE cells.
1 context sentence(design, population, supporting numbers)
Summary · context
Herein, we analyzed the expression of IGF-1 isoforms in eutopic and ectopic endometrium (red lesions and endometriotic cysts) of women with endometriosis, and we characterized the actions of a synthetic MGF E-peptide on KLE cells.
Report a problemFND-2084483404
02LabIn vitro (cells)Mechanism
Silencing of IGF-1R and insulin receptor did not alter the mitogenic action of synthetic MGF E-peptide in KLE cells.
In plain wordsIn cells, blocking the IGF-1 and insulin receptors did not stop the MGF E-peptide from making cells grow.
Peptide
MGF / PEG-MGF
Outcome
No significant changeMGF E-peptide mitogenic action after IGF-1R and insulin receptor silencing
Study
n: not reported in source
KLE cells
Comparator
Cells without receptor silencing
Outcome group
Receptor activity and mechanism · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Experimental silencing of the type 1 IGF receptor (IGF-1R) and insulin receptor expression of KLE cells (siRNA knock-out methods) did not alter the mitogenic action of the synthetic MGF E-peptide, revealing that MGF E-peptide stimulates the growth of KLE cells via an IGF-1R-independent and insulin r…
1 context sentence(design, population, supporting numbers)
Summary · context
Herein, we analyzed the expression of IGF-1 isoforms in eutopic and ectopic endometrium (red lesions and endometriotic cysts) of women with endometriosis, and we characterized the actions of a synthetic MGF E-peptide on KLE cells.
Report a problemFND-2084483405

Natural levels measured3

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

03HumanEndogenous levelObservational studyOther
In women with endometriosis, IGF-1Ec (MGF) transcripts were expressed in stromal cells of eutopic and ectopic endometrium.
In plain wordsIn women with endometriosis, the body's own MGF was present in the stromal cells of the womb lining and of lesions.
Peptide
MGF / PEG-MGF
Outcome
Not applicableIGF-1Ec (MGF) transcript expression in stromal cells of endometrium
Study
n: not reported in source
Eutopic and ectopic endometrium
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
all three igf-1 gene transcripts are expressed in the stromal cells of the eutopic and ectopic endometrium
1 context sentence(design, population, supporting numbers)
Summary · context
Herein, we analyzed the expression of IGF-1 isoforms in eutopic and ectopic endometrium (red lesions and endometriotic cysts) of women with endometriosis, and we characterized the actions of a synthetic MGF E-peptide on KLE cells.
This result measures the body's own MGF / PEG-MGF. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-2084483401
04HumanEndogenous levelObservational studyOther
In women with endometriosis, endometriotic cysts contained significantly lower expression of IGF-1 isoforms (including IGF-1Ec) at mRNA and protein level.
In plain wordsIn women with endometriosis, cysts had lower levels of the body's own IGF-1 forms, including MGF.
Peptide
MGF / PEG-MGF
Outcome
DecreaseIGF-1 isoform expression in endometriotic cysts
Study
n: not reported in source
Endometriotic cysts
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
endometriotic cysts contained significantly lower IGF-1 isoform expression, both at the mRNA and protein level, as was shown using semiquantitative PCR and immunohistochemical methods.
1 context sentence(design, population, supporting numbers)
Summary · context
Herein, we analyzed the expression of IGF-1 isoforms in eutopic and ectopic endometrium (red lesions and endometriotic cysts) of women with endometriosis, and we characterized the actions of a synthetic MGF E-peptide on KLE cells.
This result measures the body's own MGF / PEG-MGF. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-2084483402
05HumanEndogenous levelObservational studyOther
In women with endometriosis, glandular cells of ectopic endometrium (red lesions) expressed IGF-1Ec at mRNA and protein level, while eutopic glandular cells expressed no IGF-1 isoform.
In plain wordsIn women with endometriosis, gland cells in lesions made the body's own MGF, but gland cells in normal womb lining did not.
Peptide
MGF / PEG-MGF
Outcome
IncreaseIGF-1Ec expression in glandular cells of ectopic endometrium
Study
n: not reported in source
Glandular cells, red lesions
Comparator
Glandular cells of eutopic endometrium
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
In addition, the glandular cells of the eutopic endometrium did not express any of the IGF-1 isoforms; however, the glandular cells of the ectopic endometrium (red lesions) did express the IGF-1Ec at mRNA and protein level.
1 context sentence(design, population, supporting numbers)
Summary · context
Herein, we analyzed the expression of IGF-1 isoforms in eutopic and ectopic endometrium (red lesions and endometriotic cysts) of women with endometriosis, and we characterized the actions of a synthetic MGF E-peptide on KLE cells.
This result measures the body's own MGF / PEG-MGF. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-2084483403

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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