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Study · Animal study (in vivo) · mouse · 2010

13 results

AICAR treatment for 14 days normalizes obesity-induced dysregulation of TORC1 signaling and translational capacity in fasted skeletal muscle.

Drake JC, Alway SE, Hollander JM, Williamson DL

American journal of physiology. Regulatory, integrative and comparative physiology15 Sept 2010Cited by 35 (Europe PMC)

PMID20844264 (opens in a new tab)PMCIDPMC6957371 (opens in a new tab)DOI10.1152/ajpregu.00337.2010 (opens in a new tab)PepBankPUB-20844264
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study13

13 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Mechanism

    In obese mice, AICAR-treated muscle mass was higher than saline control muscle mass, independently of body weight change.

    In plain wordsIn obese mice, AICAR-treated muscle mass exceeded saline control muscle mass, independently of body weight change.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseObese muscle mass
    Study

    mousen: not reported in source

    Male lean wild-type and obese ob/ob mice
    Comparator
    Saline control
    Outcome group
    Muscle injury and repair · Musculoskeletal
    Studied dose
    0.5 mg·g body wt(-1)·day(-1) · injection · 14 daysAs given in this study (mouse).

    Measurements

    Muscle mass
    166 mg
    Obese AICAR
    Muscle mass
    159 mg
    Obese saline control

    Source sentences

    Summary
    Muscle mass was lower in OC (159 ± 12 mg) than LC, LA, and OA (176 ± 10, 178 ± 9, and 166 ± 16 mg, respectively) mice, independent of a body weight change.
    2 context sentences
    Summary · context
    Fourteen-week-old male, lean (L; 31.3 g body wt) wild-type and ob/ob (O; 59.6 g body wt) mice were injected with the AMP-activated kinase (AMPK) activator AICAR (A) at 0.5 mg·g body wt(-1)·day(-1) or saline control (C) for 14 days.
    Summary · context
    At 24 h after the last injection (including a 12-h fast), all mice were killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) was excised for analysis.
  2. 02AnimalAnimal study (in vivo)Mechanism

    In lean mice, muscle mass was 178 ± 9 mg with AICAR and 176 ± 10 mg with saline.

    In plain wordsIn lean mice, muscle mass was 178 mg with AICAR and 176 mg with saline.

    Peptide
    AICAR (acadesine)
    Outcome
    Direction not reportedLean muscle mass
    Study

    mousen: not reported in source

    Male lean wild-type and obese ob/ob mice
    Comparator
    Saline control
    Outcome group
    Muscle injury and repair · Musculoskeletal
    Studied dose
    0.5 mg·g body wt(-1)·day(-1) · injection · 14 daysAs given in this study (mouse).

    Measurements

    Muscle mass
    178 mg
    Lean AICAR
    Muscle mass
    176 mg
    Lean saline control

    Source sentences

    Summary
    Muscle mass was lower in OC (159 ± 12 mg) than LC, LA, and OA (176 ± 10, 178 ± 9, and 166 ± 16 mg, respectively) mice, independent of a body weight change.
    2 context sentences
    Summary · context
    Fourteen-week-old male, lean (L; 31.3 g body wt) wild-type and ob/ob (O; 59.6 g body wt) mice were injected with the AMP-activated kinase (AMPK) activator AICAR (A) at 0.5 mg·g body wt(-1)·day(-1) or saline control (C) for 14 days.
    Summary · context
    At 24 h after the last injection (including a 12-h fast), all mice were killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) was excised for analysis.
  3. 03AnimalAnimal study (in vivo)Mechanism

    In obese mice, AICAR normalized elevated muscle lipid staining intensity.

    In plain wordsIn obese mice, AICAR normalized the elevated lipid staining intensity in muscle compared with saline control.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseMuscle lipid staining intensity
    Study

    mousen: not reported in source

    Male lean wild-type and obese ob/ob mice
    Comparator
    Saline control
    Outcome group
    Lipids · Musculoskeletal
    Studied dose
    0.5 mg·g body wt(-1)·day(-1) · injection · 14 daysAs given in this study (mouse).

    Source sentences

    Summary
    A decrease in obese muscle mass corresponded with higher muscle cross section staining intensity for lipid and glycogen, higher blood glucose and insulin levels, and lower nuclear-enriched fractions for peroxisome proliferator-activated receptor-γ coactivator-1α protein expression in OC skeletal mus…
    2 context sentences
    Summary · context
    Fourteen-week-old male, lean (L; 31.3 g body wt) wild-type and ob/ob (O; 59.6 g body wt) mice were injected with the AMP-activated kinase (AMPK) activator AICAR (A) at 0.5 mg·g body wt(-1)·day(-1) or saline control (C) for 14 days.
    Summary · context
    At 24 h after the last injection (including a 12-h fast), all mice were killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) was excised for analysis.
  4. 04AnimalAnimal study (in vivo)Mechanism

    In obese mice, AICAR normalized elevated muscle glycogen staining intensity.

    In plain wordsIn obese mice, AICAR normalized the elevated glycogen staining intensity in muscle compared with saline control.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseMuscle glycogen staining intensity
    Study

    mousen: not reported in source

    Male lean wild-type and obese ob/ob mice
    Comparator
    Saline control
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    0.5 mg·g body wt(-1)·day(-1) · injection · 14 daysAs given in this study (mouse).

    Source sentences

    Summary
    A decrease in obese muscle mass corresponded with higher muscle cross section staining intensity for lipid and glycogen, higher blood glucose and insulin levels, and lower nuclear-enriched fractions for peroxisome proliferator-activated receptor-γ coactivator-1α protein expression in OC skeletal mus…
    2 context sentences
    Summary · context
    Fourteen-week-old male, lean (L; 31.3 g body wt) wild-type and ob/ob (O; 59.6 g body wt) mice were injected with the AMP-activated kinase (AMPK) activator AICAR (A) at 0.5 mg·g body wt(-1)·day(-1) or saline control (C) for 14 days.
    Summary · context
    At 24 h after the last injection (including a 12-h fast), all mice were killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) was excised for analysis.
  5. 05AnimalAnimal study (in vivo)Mechanism

    In obese mice, AICAR normalized elevated blood glucose levels.

    In plain wordsIn obese mice, AICAR normalized elevated blood glucose compared with saline control.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseBlood glucose
    Study

    mousen: not reported in source

    Male lean wild-type and obese ob/ob mice
    Comparator
    Saline control
    Outcome group
    Glycemic control · Metabolic and weight
    Studied dose
    0.5 mg·g body wt(-1)·day(-1) · injection · 14 daysAs given in this study (mouse).

    Source sentences

    Summary
    A decrease in obese muscle mass corresponded with higher muscle cross section staining intensity for lipid and glycogen, higher blood glucose and insulin levels, and lower nuclear-enriched fractions for peroxisome proliferator-activated receptor-γ coactivator-1α protein expression in OC skeletal mus…
    2 context sentences
    Summary · context
    Fourteen-week-old male, lean (L; 31.3 g body wt) wild-type and ob/ob (O; 59.6 g body wt) mice were injected with the AMP-activated kinase (AMPK) activator AICAR (A) at 0.5 mg·g body wt(-1)·day(-1) or saline control (C) for 14 days.
    Summary · context
    At 24 h after the last injection (including a 12-h fast), all mice were killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) was excised for analysis.
  6. 06AnimalAnimal study (in vivo)Mechanism

    In obese mice, AICAR normalized elevated blood insulin levels.

    In plain wordsIn obese mice, AICAR normalized elevated blood insulin compared with saline control.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseBlood insulin
    Study

    mousen: not reported in source

    Male lean wild-type and obese ob/ob mice
    Comparator
    Saline control
    Outcome group
    Glycemic control · Metabolic and weight
    Studied dose
    0.5 mg·g body wt(-1)·day(-1) · injection · 14 daysAs given in this study (mouse).

    Source sentences

    Summary
    A decrease in obese muscle mass corresponded with higher muscle cross section staining intensity for lipid and glycogen, higher blood glucose and insulin levels, and lower nuclear-enriched fractions for peroxisome proliferator-activated receptor-γ coactivator-1α protein expression in OC skeletal mus…
    2 context sentences
    Summary · context
    Fourteen-week-old male, lean (L; 31.3 g body wt) wild-type and ob/ob (O; 59.6 g body wt) mice were injected with the AMP-activated kinase (AMPK) activator AICAR (A) at 0.5 mg·g body wt(-1)·day(-1) or saline control (C) for 14 days.
    Summary · context
    At 24 h after the last injection (including a 12-h fast), all mice were killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) was excised for analysis.
  7. 07AnimalAnimal study (in vivo)Mechanism

    In obese mouse muscle, AICAR normalized reduced nuclear-enriched PGC-1α protein expression.

    In plain wordsIn obese mice, AICAR normalized reduced nuclear PGC-1α protein in muscle compared with saline control.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseNuclear PGC-1α protein
    Study

    mousen: not reported in source

    Male lean wild-type and obese ob/ob mice
    Comparator
    Saline control
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    0.5 mg·g body wt(-1)·day(-1) · injection · 14 daysAs given in this study (mouse).

    Source sentences

    Summary
    A decrease in obese muscle mass corresponded with higher muscle cross section staining intensity for lipid and glycogen, higher blood glucose and insulin levels, and lower nuclear-enriched fractions for peroxisome proliferator-activated receptor-γ coactivator-1α protein expression in OC skeletal mus…
    2 context sentences
    Summary · context
    Fourteen-week-old male, lean (L; 31.3 g body wt) wild-type and ob/ob (O; 59.6 g body wt) mice were injected with the AMP-activated kinase (AMPK) activator AICAR (A) at 0.5 mg·g body wt(-1)·day(-1) or saline control (C) for 14 days.
    Summary · context
    At 24 h after the last injection (including a 12-h fast), all mice were killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) was excised for analysis.
  8. 08AnimalAnimal study (in vivo)Mechanism

    In obese mice, AICAR augmented reduced muscle AMPK phosphorylation.

    In plain wordsIn obese mice, AICAR increased muscle AMPK phosphorylation compared with saline control.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseAMPK phosphorylation
    Study

    mousen: not reported in source

    Male lean wild-type and obese ob/ob mice
    Comparator
    Saline control
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    0.5 mg·g body wt(-1)·day(-1) · injection · 14 daysAs given in this study (mouse).

    Source sentences

    Summary
    AMPK and acetyl-cocarboxylase phosphorylation was reduced in OC mice and augmented by AICAR treatment in OA mice.
    2 context sentences
    Summary · context
    Fourteen-week-old male, lean (L; 31.3 g body wt) wild-type and ob/ob (O; 59.6 g body wt) mice were injected with the AMP-activated kinase (AMPK) activator AICAR (A) at 0.5 mg·g body wt(-1)·day(-1) or saline control (C) for 14 days.
    Summary · context
    At 24 h after the last injection (including a 12-h fast), all mice were killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) was excised for analysis.
  9. 09AnimalAnimal study (in vivo)Mechanism

    In obese mice, AICAR augmented reduced muscle acetyl-cocarboxylase phosphorylation.

    In plain wordsIn obese mice, AICAR increased muscle acetyl-cocarboxylase phosphorylation compared with saline control.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseAcetyl-cocarboxylase phosphorylation
    Study

    mousen: not reported in source

    Male lean wild-type and obese ob/ob mice
    Comparator
    Saline control
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    0.5 mg·g body wt(-1)·day(-1) · injection · 14 daysAs given in this study (mouse).

    Source sentences

    Summary
    AMPK and acetyl-cocarboxylase phosphorylation was reduced in OC mice and augmented by AICAR treatment in OA mice.
    2 context sentences
    Summary · context
    Fourteen-week-old male, lean (L; 31.3 g body wt) wild-type and ob/ob (O; 59.6 g body wt) mice were injected with the AMP-activated kinase (AMPK) activator AICAR (A) at 0.5 mg·g body wt(-1)·day(-1) or saline control (C) for 14 days.
    Summary · context
    At 24 h after the last injection (including a 12-h fast), all mice were killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) was excised for analysis.
  10. 10AnimalAnimal study (in vivo)Mechanism

    In obese mouse muscle, AICAR reversed elevated S6 kinase-1 activation.

    In plain wordsIn obese mice, AICAR reduced elevated muscle S6 kinase-1 activation compared with saline control.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseS6 kinase-1 activation
    Study

    mousen: not reported in source

    Male lean wild-type and obese ob/ob mice
    Comparator
    Saline control
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    0.5 mg·g body wt(-1)·day(-1) · injection · 14 daysAs given in this study (mouse).

    Source sentences

    Summary
    Conversely, OC mice displayed higher activation of downstream targets (S6 kinase-1 and ribosomal protein S6) of mTOR and lower raptor-associated mTOR than LC mice, which were reciprocally altered after 14 days of AICAR treatment.
    2 context sentences
    Summary · context
    Fourteen-week-old male, lean (L; 31.3 g body wt) wild-type and ob/ob (O; 59.6 g body wt) mice were injected with the AMP-activated kinase (AMPK) activator AICAR (A) at 0.5 mg·g body wt(-1)·day(-1) or saline control (C) for 14 days.
    Summary · context
    At 24 h after the last injection (including a 12-h fast), all mice were killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) was excised for analysis.
  11. 11AnimalAnimal study (in vivo)Mechanism

    In obese mouse muscle, AICAR reversed elevated ribosomal protein S6 activation.

    In plain wordsIn obese mice, AICAR reduced elevated ribosomal protein S6 activation compared with saline control.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseRibosomal protein S6 activation
    Study

    mousen: not reported in source

    Male lean wild-type and obese ob/ob mice
    Comparator
    Saline control
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    0.5 mg·g body wt(-1)·day(-1) · injection · 14 daysAs given in this study (mouse).

    Source sentences

    Summary
    Conversely, OC mice displayed higher activation of downstream targets (S6 kinase-1 and ribosomal protein S6) of mTOR and lower raptor-associated mTOR than LC mice, which were reciprocally altered after 14 days of AICAR treatment.
    2 context sentences
    Summary · context
    Fourteen-week-old male, lean (L; 31.3 g body wt) wild-type and ob/ob (O; 59.6 g body wt) mice were injected with the AMP-activated kinase (AMPK) activator AICAR (A) at 0.5 mg·g body wt(-1)·day(-1) or saline control (C) for 14 days.
    Summary · context
    At 24 h after the last injection (including a 12-h fast), all mice were killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) was excised for analysis.
  12. 12AnimalAnimal study (in vivo)Mechanism

    In obese mouse muscle, AICAR reversed reduced raptor-associated mTOR.

    In plain wordsIn obese mice, AICAR increased reduced raptor-associated mTOR in muscle compared with saline control.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseRaptor-associated mTOR
    Study

    mousen: not reported in source

    Male lean wild-type and obese ob/ob mice
    Comparator
    Saline control
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    0.5 mg·g body wt(-1)·day(-1) · injection · 14 daysAs given in this study (mouse).

    Source sentences

    Summary
    Conversely, OC mice displayed higher activation of downstream targets (S6 kinase-1 and ribosomal protein S6) of mTOR and lower raptor-associated mTOR than LC mice, which were reciprocally altered after 14 days of AICAR treatment.
    2 context sentences
    Summary · context
    Fourteen-week-old male, lean (L; 31.3 g body wt) wild-type and ob/ob (O; 59.6 g body wt) mice were injected with the AMP-activated kinase (AMPK) activator AICAR (A) at 0.5 mg·g body wt(-1)·day(-1) or saline control (C) for 14 days.
    Summary · context
    At 24 h after the last injection (including a 12-h fast), all mice were killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) was excised for analysis.
  13. 13AnimalAnimal study (in vivo)Mechanism

    In obese mouse muscle, AICAR improved dysregulated translational capacity, assessed through ribosome fractionation, RNA content, eIF4F formation and eIF4G phosphorylation.

    In plain wordsIn obese mice, AICAR improved abnormal muscle translation capacity compared with saline control.

    Peptide
    AICAR (acadesine)
    Outcome
    Direction not reportedTranslational capacity
    Study

    mousen: not reported in source

    Male lean wild-type and obese ob/ob mice
    Comparator
    Saline control
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    0.5 mg·g body wt(-1)·day(-1) · injection · 14 daysAs given in this study (mouse).

    Source sentences

    Summary
    Dysregulation of translational capacity was improved in OA mice, as assessed by sucrose density gradient fractionation of ribosomes, total and ribosome-associated RNA content, eukaryotic initiation factor 4F complex formation, and eukaryotic initiation factor 4G phosphorylation.
    2 context sentences
    Summary · context
    Fourteen-week-old male, lean (L; 31.3 g body wt) wild-type and ob/ob (O; 59.6 g body wt) mice were injected with the AMP-activated kinase (AMPK) activator AICAR (A) at 0.5 mg·g body wt(-1)·day(-1) or saline control (C) for 14 days.
    Summary · context
    At 24 h after the last injection (including a 12-h fast), all mice were killed, and the plantar flexor complex muscle (gastrocnemius, soleus, and plantaris) was excised for analysis.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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