In COS cells, receptor Cys residues at positions 116 and 198 and Glu¹⁸⁷ were critical for GHRP‑6 biological activity.
In plain wordsIn cells, changes to specific receptor residues showed their importance for GHRP‑6 activity.
- Peptide
- GHRP-6
- Outcome
- Not applicableReceptor residues critical for biological activity
- Study
n: not reported in source
COS cells expressing mutated human GHS receptor 1a- Outcome group
- Receptor activity and mechanism · Pharmacology
Source sentences
Cys residues in positions 116 and 198 in the first and second extracellular loops and the perimembranous Glu¹⁸⁷ residue in the second extracellular loop were critical for ghrelin and GHRP-6 biological activity.
1 context sentence(design, population, supporting numbers)
Each construct was transiently expressed in COS cells, and characterized for ghrelin- and growth hormone releasing peptide (GHRP)-6-stimulated intracellular calcium responses and ghrelin radioligand binding.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.