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Study · In vitro (cell-free) · rat · 1982

9 results

Differences in prolyl-leucyl-glycinamide metabolism by hypothalamus, pituitary and cerebral cortex of male and female rats.

Celis ME, Peña C

Neurochemistry international1 Jan 1982

PMID20487848 (opens in a new tab)DOI10.1016/0197-0186(82)90024-9 (opens in a new tab)PepBankPUB-20487848
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study9

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cell-free)Pharmacokinetics

    In rat tissue homogenates, PLG underwent rapid hydrolysis in hypothalamic, pituitary and cortical preparations from both sexes.

    In plain wordsIn lab tests, rat hypothalamic, pituitary and cortical preparations from both sexes rapidly broke down PLG.

    Peptide
    MIF-1 (Pro-Leu-Gly-NH2)
    Outcome
    Not applicablePLG hydrolysis
    Study

    ratn: not reported in source

    Rat tissue homogenates
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    l-Prolyl-l-leucyl-glycinamide is rapidly hydrolyzed by hypothalamic, hypophyseal and cortical homogenates from male or female rats.
  2. 02LabIn vitro (cell-free)Pharmacokinetics

    In rat homogenates, PLG-degrading activity was highest in pituitary, followed by hypothalamus and cerebral cortex.

    In plain wordsIn lab tests, rat pituitary broke down PLG most actively, followed by hypothalamus and cerebral cortex.

    Peptide
    MIF-1 (Pro-Leu-Gly-NH2)
    Outcome
    MixedTissue ranking of PLG hydrolysis
    Study

    ratn: not reported in source

    Rat tissue homogenates
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The peptidase activity is higher in the pituitary followed in decreasing order by the hypothalamus and the cerebral cortex.
    1 context sentence
    Summary · context
    l-Prolyl-l-leucyl-glycinamide is rapidly hydrolyzed by hypothalamic, hypophyseal and cortical homogenates from male or female rats.
  3. 03LabIn vitro (cell-free)Pharmacokinetics

    In rat tissues, PLG-degrading activity in females was half that in males.

    In plain wordsIn lab tests, female rat tissues broke down PLG half as actively as male rat tissues.

    Peptide
    MIF-1 (Pro-Leu-Gly-NH2)
    Outcome
    DecreaseSex difference in PLG metabolism
    Study

    ratn: not reported in source

    Rat tissue homogenates
    Comparator
    Male rat tissues
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Tissues from female rats are half as active as those from male rats and show variations during the estrous cycle, with very low PLG metabolism at diestrus 1 in pituitary and hypothalamus.
  4. 04LabIn vitro (cell-free)Pharmacokinetics

    In female rat tissues, PLG metabolism varied during the estrous cycle and was very low at diestrus 1 in pituitary and hypothalamus.

    In plain wordsIn lab tests, PLG breakdown varied across the rat estrous cycle, with very low pituitary and hypothalamic activity at diestrus 1.

    Peptide
    MIF-1 (Pro-Leu-Gly-NH2)
    Outcome
    MixedEstrous-cycle variation in pituitary and hypothalamic PLG metabolism
    Study

    ratn: not reported in source

    Rat tissue homogenates
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Tissues from female rats are half as active as those from male rats and show variations during the estrous cycle, with very low PLG metabolism at diestrus 1 in pituitary and hypothalamus.
  5. 05LabIn vitro (cell-free)Pharmacokinetics

    In female rat cerebral cortex, PLG hydrolysis was significantly lower at proestrus and estrus than at diestrus.

    In plain wordsIn lab tests, rat cortex broke down PLG less at proestrus and estrus than at diestrus.

    Peptide
    MIF-1 (Pro-Leu-Gly-NH2)
    Outcome
    DecreaseCortical PLG hydrolysis across estrous stages
    Study

    ratn: not reported in source

    Rat tissue homogenates
    Comparator
    Diestrus
    Outcome group
    Pharmacokinetics · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In contrast, the cerebral cortex at proestrus and estrus has significant lower hydrolyzing activity than at diestrus.
    1 context sentence
    Summary · context
    l-Prolyl-l-leucyl-glycinamide is rapidly hydrolyzed by hypothalamic, hypophyseal and cortical homogenates from male or female rats.

Studies that did not test the peptide alone4

In this study, MIF-1 (Pro-Leu-Gly-NH2) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking MIF-1 (Pro-Leu-Gly-NH2).

  1. 06LabNot a test of MIF-1 (Pro-Leu-Gly-NH2) aloneIn vitro (cell-free)Mechanism

    In rat tissue preparations, PLG-degrading activity was mostly in the supernatant fraction after centrifugation.

    In plain wordsIn lab tests, most PLG breakdown activity remained in the liquid fraction after rat tissue preparations underwent centrifugation.

    Peptide
    MIF-1 (Pro-Leu-Gly-NH2)
    Outcome
    Not applicableSubcellular localization of PLG peptidase activity
    Study

    ratn: not reported in source

    Rat tissue homogenates
    Outcome group
    Pharmacokinetics · Pharmacology

    Source sentences

    Summary
    It is mostly localized in the supernatant fraction of a 100,000 g centrifugation and is inhibited by bacitracin.
    1 context sentence
    Summary · context
    l-Prolyl-l-leucyl-glycinamide is rapidly hydrolyzed by hypothalamic, hypophyseal and cortical homogenates from male or female rats.

    In this study, MIF-1 (Pro-Leu-Gly-NH2) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MIF-1 (Pro-Leu-Gly-NH2).

  2. 07LabNot a test of MIF-1 (Pro-Leu-Gly-NH2) aloneIn vitro (cell-free)Mechanism

    In rat tissue preparations, bacitracin inhibited PLG-degrading activity.

    In plain wordsIn lab tests, bacitracin reduced PLG breakdown activity in rat tissue preparations.

    Peptide
    MIF-1 (Pro-Leu-Gly-NH2)
    Outcome
    DecreaseBacitracin inhibition of PLG degradation
    Study

    ratn: not reported in source

    Rat tissue homogenates
    Outcome group
    Pharmacokinetics · Pharmacology

    Source sentences

    Summary
    It is mostly localized in the supernatant fraction of a 100,000 g centrifugation and is inhibited by bacitracin.
    1 context sentence
    Summary · context
    l-Prolyl-l-leucyl-glycinamide is rapidly hydrolyzed by hypothalamic, hypophyseal and cortical homogenates from male or female rats.

    In this study, MIF-1 (Pro-Leu-Gly-NH2) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MIF-1 (Pro-Leu-Gly-NH2).

  3. 08LabNot a test of MIF-1 (Pro-Leu-Gly-NH2) aloneIn vitro (cell-free)Pharmacokinetics

    In tissues from ovariectomized rats, estrogen treatment did not change PLG-degrading activity.

    In plain wordsIn lab tests, tissues from ovariectomized rats showed no change in PLG breakdown activity after estrogen treatment.

    Peptide
    MIF-1 (Pro-Leu-Gly-NH2)
    Outcome
    No significant changeEstrogen effect on PLG peptidase activity
    Study

    ratn: not reported in source

    Rat tissue homogenates
    Outcome group
    Pharmacokinetics · Pharmacology

    Source sentences

    Summary
    No change of the peptidase activity is observed in tissues from ovariectomized animals after treatment with estrogen or progesterone.
    1 context sentence
    Summary · context
    l-Prolyl-l-leucyl-glycinamide is rapidly hydrolyzed by hypothalamic, hypophyseal and cortical homogenates from male or female rats.

    In this study, MIF-1 (Pro-Leu-Gly-NH2) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MIF-1 (Pro-Leu-Gly-NH2).

  4. 09LabNot a test of MIF-1 (Pro-Leu-Gly-NH2) aloneIn vitro (cell-free)Pharmacokinetics

    In tissues from ovariectomized rats, progesterone treatment did not change PLG-degrading activity.

    In plain wordsIn lab tests, tissues from ovariectomized rats showed no change in PLG breakdown activity after progesterone treatment.

    Peptide
    MIF-1 (Pro-Leu-Gly-NH2)
    Outcome
    No significant changeProgesterone effect on PLG peptidase activity
    Study

    ratn: not reported in source

    Rat tissue homogenates
    Outcome group
    Pharmacokinetics · Pharmacology

    Source sentences

    Summary
    No change of the peptidase activity is observed in tissues from ovariectomized animals after treatment with estrogen or progesterone.
    1 context sentence
    Summary · context
    l-Prolyl-l-leucyl-glycinamide is rapidly hydrolyzed by hypothalamic, hypophyseal and cortical homogenates from male or female rats.

    In this study, MIF-1 (Pro-Leu-Gly-NH2) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MIF-1 (Pro-Leu-Gly-NH2).

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    22 Oct 2026, 02:26 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 18:59 UTC

    First added · source update from Europe PMC

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