[The role of collagen implants containing the tripeptide gly-his-lys in bone healing process.].

Adam M, Cech O, Pohunková H, Stehlík J, Klézl Z

Acta chirurgiae orthopaedicae et traumatologiae Cechoslovaca1 Jan 1995

PMID20470489PepBankPUB-20470489

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

1 result from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In minipigs, gel with GHK healed bone defects substantially faster than the same gel without GHK, requiring approximately half the time less.
In plain wordsIn minipigs, gel with GHK healed bone defects faster than the same gel without GHK.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
DecreaseBone defect healing time
Study
minipign: not reported in source
femoral bone defects
Comparator
Gel containing peflacin and HSGAG without GHK
Outcome group
Bone and joint · Musculoskeletal
Studied dose
Dose not stated
Source sentences
Summary
The authors found out that slowest healing was in case of unfilled defects, on the other hand defects filled with collagen gel and GHK were healing substantially faster than defects filled with gel without GHK, i.e. healing needs approximately half the time less.
3 context sentences(design, population, supporting numbers)
Summary · context
Healing was followed up both histologically and with help of RTG and NMR and compared with healing of defects either unfilled, or filled with gel containing peflacin and HSGAG, but not tripeptide.
Summary · context
The gel was used in a model experiment for filling artifically created bone defects in femurs of minipigs.
Summary · context
The authors prepared 12,5% collagen gel, that they supplemented by tripeptide gly-his-lys. peflacin and hypersulfated glycosaminoglycan (HSGAG).
Report a problemFND-2047048905

Studies that did not test the peptide alone5

In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHK (glycyl-L-histidyl-L-lysine).

02AnimalNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneAnimal study (in vivo)Other
In rats, absorption of the combination collagen gel without HSGAG was faster than absorption with HSGAG.
In plain wordsIn rats, the combination gel without HSGAG absorbed faster than gel with HSGAG.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
IncreaseGel absorption
Study
ratn: not reported in source
subcutaneous polyurethane sponges
Comparator
Gel with HSGAG
Outcome group
Pharmacokinetics · Pharmacology
Source sentences
Summary
They found out, that absorption of gel without HSGAG is faster.
2 context sentences(design, population, supporting numbers)
Summary · context
By means of I125 marking they followed up its absorption from small polyurethan sponges placed under the skin of rats.
Summary · context
The authors prepared 12,5% collagen gel, that they supplemented by tripeptide gly-his-lys. peflacin and hypersulfated glycosaminoglycan (HSGAG).
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-2047048901
03AnimalNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneAnimal study (in vivo)Safety outcome
In rabbits, the combination gel produced no antibodies against collagen types I, II or III.
In plain wordsIn rabbits, the combination gel produced no antibodies against the reported collagen types.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
Not applicableCollagen antibodies
Study
rabbitn: not reported in source
gel implantation
Outcome group
Immune response · Immune and inflammation
Source sentences
Summary
No collagen antibodies (type I., II., III.) against the gel were formed neither in the experiment with rabbits, nor later on with minipigs when gel was placed into bone defects.
1 context sentence(design, population, supporting numbers)
Summary · context
The authors prepared 12,5% collagen gel, that they supplemented by tripeptide gly-his-lys. peflacin and hypersulfated glycosaminoglycan (HSGAG).
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-2047048902
04AnimalNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneAnimal study (in vivo)Safety outcome
In minipigs with bone defects, the combination gel produced no antibodies against collagen types I, II or III.
In plain wordsIn minipigs with bone defects, the combination gel produced no antibodies against the reported collagen types.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
Not applicableCollagen antibodies
Study
minipign: not reported in source
bone defects
Outcome group
Immune response · Immune and inflammation
Source sentences
Summary
No collagen antibodies (type I., II., III.) against the gel were formed neither in the experiment with rabbits, nor later on with minipigs when gel was placed into bone defects.
1 context sentence(design, population, supporting numbers)
Summary · context
The authors prepared 12,5% collagen gel, that they supplemented by tripeptide gly-his-lys. peflacin and hypersulfated glycosaminoglycan (HSGAG).
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-2047048903
05AnimalNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneAnimal study (in vivo)Efficacy outcome
In minipigs, defects filled with combination collagen gel containing GHK healed faster than unfilled defects, which healed slowest.
In plain wordsIn minipigs, defects filled with combination gel containing GHK healed faster than unfilled defects.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
IncreaseBone defect healing
Study
minipign: not reported in source
femoral bone defects
Comparator
Unfilled defects
Outcome group
Bone and joint · Musculoskeletal
Source sentences
Summary
The authors found out that slowest healing was in case of unfilled defects, on the other hand defects filled with collagen gel and GHK were healing substantially faster than defects filled with gel without GHK, i.e. healing needs approximately half the time less.
2 context sentences(design, population, supporting numbers)
Summary · context
The gel was used in a model experiment for filling artifically created bone defects in femurs of minipigs.
Summary · context
The authors prepared 12,5% collagen gel, that they supplemented by tripeptide gly-his-lys. peflacin and hypersulfated glycosaminoglycan (HSGAG).
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-2047048906
06LabNot a test of GHK (glycyl-L-histidyl-L-lysine) aloneIn vitro (cell-free)Other
In bacteriological nutrient media, tests detected no microorganisms in the combination gel.
In plain wordsIn lab tests, no microorganisms were detected in the combination gel.
Peptide
GHK (glycyl-L-histidyl-L-lysine)
Outcome
Not applicableMicroorganism detection
Study
n: not reported in source
bacteriological nutrient media
Outcome group
Infection and antimicrobial activity · Infection
Source sentences
Summary
Further on presence of microorganisms in the gel was looked for with negative results on various bacteriological nutrient media.
1 context sentence(design, population, supporting numbers)
Summary · context
The authors prepared 12,5% collagen gel, that they supplemented by tripeptide gly-his-lys. peflacin and hypersulfated glycosaminoglycan (HSGAG).
In this study, GHK (glycyl-L-histidyl-L-lysine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHK (glycyl-L-histidyl-L-lysine).
Report a problemFND-2047048904

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
14 Oct 2026, 05:02 UTC
Study results updated
Version 1
14 Oct 2026, 01:46 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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