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Study · In vitro (cells) · 2010

7 results

GHRP-6 induces CREB phosphorylation and growth hormone secretion via a protein kinase Csigma-dependent pathway in GH3 cells.

Tian C, Ye F, Xu T, Wang S, Wang X, Wang H, Wan F, Lei T

Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban21 Apr 2010Cited by 9 (Europe PMC)

PMID20407870 (opens in a new tab)DOI10.1007/s11596-010-0210-5 (opens in a new tab)PepBankPUB-20407870
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Efficacy outcome

    In GH3 cells, GHRP‑6 stimulated GH secretion in time- and dose-dependent manners.

    In plain wordsIn cells, GHRP‑6 increased growth hormone release with time and dose.

    Peptide
    GHRP-6
    Outcome
    IncreaseGH secretion
    Study

    n: not reported in source

    GH3 cells
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The results showed that GHRP-6 stimulated GH secretion in both time- and dose-dependent manners
    1 context sentence
    Summary · context
    This study examined the effect of GHRP-6, a known GHSs receptor agonist, on the phosphorylation of cAMP-responsive element-binding protein (CREB) and the underly mechanism.
  2. 02LabIn vitro (cells)Efficacy outcome

    In GH3 cells, GHRP‑6 enhanced the effect of GHRH on GH secretion.

    In plain wordsIn cells, GHRP‑6 increased growth hormone release beyond the effect of GHRH alone.

    Peptide
    GHRP-6
    Outcome
    IncreaseGHRH-stimulated GH secretion
    Study

    n: not reported in source

    GH3 cells
    Comparator
    GHRH alone
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The results showed that GHRP-6 stimulated GH secretion in both time- and dose-dependent manners and enhanced the effect of GHRH on GH secretion.
    1 context sentence
    Summary · context
    This study examined the effect of GHRP-6, a known GHSs receptor agonist, on the phosphorylation of cAMP-responsive element-binding protein (CREB) and the underly mechanism.
  3. 03LabIn vitro (cells)Mechanism

    In GH3 cells, GHRP‑6 induced CREB phosphorylation.

    In plain wordsIn cells, GHRP‑6 increased CREB phosphorylation.

    Peptide
    GHRP-6
    Outcome
    IncreaseCREB phosphorylation
    Study

    n: not reported in source

    GH3 cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    GHRP-6 was also found to induce CREB phosphorylation.
    1 context sentence
    Summary · context
    This study examined the effect of GHRP-6, a known GHSs receptor agonist, on the phosphorylation of cAMP-responsive element-binding protein (CREB) and the underly mechanism.
  4. 04LabIn vitro (cells)Mechanism

    In GH3 cells, GHRP‑6 activated PKCsigma.

    In plain wordsIn cells, GHRP‑6 activated PKCsigma.

    Peptide
    GHRP-6
    Outcome
    IncreasePKCsigma activation
    Study

    n: not reported in source

    GH3 cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    PKCsigma could be activated by GHRP-6.
    1 context sentence
    Summary · context
    This study examined the effect of GHRP-6, a known GHSs receptor agonist, on the phosphorylation of cAMP-responsive element-binding protein (CREB) and the underly mechanism.

Studies that did not test the peptide alone3

In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-6.

  1. 05LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism

    In GH3 cells tested with GHRP‑6, the PKC activator PMA enhanced GH secretion.

    In plain wordsIn cells tested with GHRP‑6, PMA increased growth hormone release.

    Peptide
    GHRP-6
    Outcome
    IncreaseGH secretion with PKC activation
    Study

    n: not reported in source

    GH3 cells
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    Moreover, GH secretion was enhanced by the PKC activator PMA
    2 context sentences
    Summary · context
    GH3 cells were cultured and subjected to different treatments as follows: GHRP-6, GHRP-6 plus GHRH, phorbol ester (PMA), an activator of PKC, alone or in combination with GHRP-6, Gö6983, a general inhibitor of PKCs, in the presence or absence of GHRP-6, rottlerin, an inhibitor of PKCs, alone or plus GHRP-6.
    Summary · context
    This study examined the effect of GHRP-6, a known GHSs receptor agonist, on the phosphorylation of cAMP-responsive element-binding protein (CREB) and the underly mechanism.

    In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.

  2. 06LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism

    In GH3 cells tested with GHRP‑6, PKC inhibitors reduced GH secretion.

    In plain wordsIn cells tested with GHRP‑6, PKC inhibitors reduced growth hormone release.

    Peptide
    GHRP-6
    Outcome
    DecreaseGH secretion with PKC inhibition
    Study

    n: not reported in source

    GH3 cells
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    Moreover, GH secretion was enhanced by the PKC activator PMA and reduced by the PKC inhibitors (Gö6983, rottlerin) and knockdown of PKCsigma.
    2 context sentences
    Summary · context
    GH3 cells were cultured and subjected to different treatments as follows: GHRP-6, GHRP-6 plus GHRH, phorbol ester (PMA), an activator of PKC, alone or in combination with GHRP-6, Gö6983, a general inhibitor of PKCs, in the presence or absence of GHRP-6, rottlerin, an inhibitor of PKCs, alone or plus GHRP-6.
    Summary · context
    This study examined the effect of GHRP-6, a known GHSs receptor agonist, on the phosphorylation of cAMP-responsive element-binding protein (CREB) and the underly mechanism.

    In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.

  3. 07LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism

    In GHRP‑6-treated GH3 cells, PKCsigma knockdown reduced GH secretion.

    In plain wordsIn cells given GHRP‑6, PKCsigma gene knockdown reduced growth hormone release.

    Peptide
    GHRP-6
    Outcome
    DecreaseGH secretion with PKCsigma knockdown
    Study

    n: not reported in source

    GH3 cells
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    Moreover, GH secretion was enhanced by the PKC activator PMA and reduced by the PKC inhibitors (Gö6983, rottlerin) and knockdown of PKCsigma.
    2 context sentences
    Summary · context
    The cells were transiently transfected with PKCsigma-specific siRNA and then treated with GHRP-6.
    Summary · context
    This study examined the effect of GHRP-6, a known GHSs receptor agonist, on the phosphorylation of cAMP-responsive element-binding protein (CREB) and the underly mechanism.

    In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:13 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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