Expression of IGF-1 isoforms after exercise-induced muscle damage in humans: characterization of the MGF E peptide actions in vitro.

Philippou A, Papageorgiou E, Bogdanis G, Halapas A, Sourla A, Maridaki M, Pissimissis N, Koutsilieris M

In vivo (Athens, Greece)1 Jul 2009Cited by 80 (Europe PMC)

PMID19567392PepBankPUB-19567392

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study4

No result from this study comes from a test of the peptide.

Natural levels measured2

These results measure the body's own peptide. Nobody was given the peptide. They are not evidence about taking it. They are left out of key results, evidence counts and charts.

01HumanEndogenous levelNon-randomized trialOther
In healthy men after exercise-induced muscle damage, muscle MGF mRNA showed a rapid and transient rise.
In plain wordsIn healthy men, the body's own MGF mRNA in muscle rose briefly after muscle-damaging exercise.
Peptide
MGF / PEG-MGF
Outcome
IncreaseMGF mRNA after exercise-induced muscle damage
Study
n = 10
Outcome group
Growth hormone and IGF-1 · Musculoskeletal
Source sentences
Summary
PCR-based analyses of muscle biopsy samples revealed a rapid and transient up-regulation of MGF mRNA expression which was followed by a prolonged increase of IGF-1Ea and IGF-1Eb mRNA expression (p<0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Ten healthy male volunteers were subjected to exercise-induced muscle damage and biopsy samples were taken from the exercised muscles before and 6 h, 2, 5 and 16 days post exercise.
This result measures the body's own MGF / PEG-MGF. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-1956739201
02HumanEndogenous levelNon-randomized trialOther
In healthy men after exercise-induced muscle damage, MGF protein expression followed a pattern similar to that of its mRNA.
In plain wordsIn healthy men, the body's own MGF protein in muscle followed a pattern similar to its mRNA after muscle-damaging exercise.
Peptide
MGF / PEG-MGF
Outcome
IncreaseMGF protein after exercise-induced muscle damage
Study
n = 10
Outcome group
Growth hormone and IGF-1 · Musculoskeletal
Source sentences
Summary
Patterns similar to those for mRNA expression were detected for MGF and IGF-1Ea expression at the protein level.
1 context sentence(design, population, supporting numbers)
Summary · context
Ten healthy male volunteers were subjected to exercise-induced muscle damage and biopsy samples were taken from the exercised muscles before and 6 h, 2, 5 and 16 days post exercise.
This result measures the body's own MGF / PEG-MGF. Nobody was given the peptide. It is not evidence about taking it.
Report a problemFND-1956739202

Studies that did not test the peptide alone2

In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking MGF / PEG-MGF.

03LabNot a test of MGF / PEG-MGF aloneIn vitro (cells)Mechanism
In C2C12 myoblast-like cells, the proliferative effect of synthetic MGF E peptide was not blocked by an anti-IGF-1 receptor neutralizing antibody.
In plain wordsIn muscle-like cells, blocking the IGF-1 receptor did not stop the proliferative effect of the MGF E peptide.
Peptide
MGF / PEG-MGF
Outcome
Not applicableEffect of anti-IGF-1R antibody on synthetic MGF E peptide proliferative effect
Study
n: not reported in source
C2C12 myoblast-like cells
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
The action of synthetic MGF E peptide differed from that of mature IGF-1 since its proliferative effect on C2C12 myoblast-like cells was not blocked by an anti-IGF-1 receptor neutralizing antibody and it did not phosphorylate Akt.
1 context sentence(design, population, supporting numbers)
Summary · context
In addition, we characterized mature IGF-1 and synthetic MGF E peptide signalling in C2C12 myoblast-like cells in vitro.
In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.
Report a problemFND-1956739203
04LabNot a test of MGF / PEG-MGF aloneIn vitro (cells)Mechanism
In C2C12 myoblast-like cells, synthetic MGF E peptide did not phosphorylate Akt.
In plain wordsIn muscle-like cells, the MGF E peptide did not switch on the Akt signal.
Peptide
MGF / PEG-MGF
Outcome
No significant changeAkt phosphorylation by synthetic MGF E peptide
Study
n: not reported in source
C2C12 myoblast-like cells
Outcome group
Receptor activity and mechanism · Musculoskeletal
Source sentences
Summary
The action of synthetic MGF E peptide differed from that of mature IGF-1 since its proliferative effect on C2C12 myoblast-like cells was not blocked by an anti-IGF-1 receptor neutralizing antibody and it did not phosphorylate Akt.
1 context sentence(design, population, supporting numbers)
Summary · context
In addition, we characterized mature IGF-1 and synthetic MGF E peptide signalling in C2C12 myoblast-like cells in vitro.
In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.
Report a problemFND-1956739204

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

Journal Article

MeSH terms

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