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Study · Not a test of the peptide alone · 2009

2 results

RimJ is responsible for N(alpha)-acetylation of thymosin alpha1 in Escherichia coli.

Fang H, Zhang X, Shen L, Si X, Ren Y, Dai H, Li S, Zhou C, Chen H

Applied microbiology and biotechnology8 Apr 2009Cited by 19 (Europe PMC)

PMID19352641 (opens in a new tab)DOI10.1007/s00253-009-1994-8 (opens in a new tab)PepBankPUB-19352641
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone2

In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin alpha-1.

  1. 01LabNot a test of Thymosin alpha-1 aloneIn vitro (cells)Mechanism

    In E. coli, disruption of rimJ left the thymosin alpha1 fusion protein completely unacetylated.

    In plain wordsIn lab bacteria, removing the rimJ enzyme gene stopped the thymosin alpha1 fusion protein from being acetylated.

    Peptide
    Thymosin alpha-1
    Outcome
    DecreaseN-alpha-acetylation of thymosin alpha1 fusion protein when rimJ is disrupted
    Study

    Escherichia colin: not reported in source

    recombinant thymosin alpha1-L12 fusion protein in E. coli
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    Through deletion of some N(alpha)-acetyltransferases by Red recombination, we found that, when rimJ is disrupted, the fusion protein is completely unacetylated.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

  2. 02LabNot a test of Thymosin alpha-1 aloneIn vitro (cells)Mechanism

    In E. coli, RimJ catalyzes N-alpha-acetylation of recombinant Talpha1-fused protein, and co-expression with RimJ gives fully acetylated Talpha1.

    In plain wordsIn lab bacteria, the RimJ enzyme adds the acetyl group to thymosin alpha1, and producing both together gives fully acetylated peptide.

    Peptide
    Thymosin alpha-1
    Outcome
    IncreaseFull N-alpha-acetylation of recombinant thymosin alpha1 by RimJ co-expression
    Study

    Escherichia colin: not reported in source

    recombinant thymosin alpha1-L12 fusion protein in E. coli
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    Our results demonstrate that N(alpha)-acetylation of recombinant Talpha1-fused protein in E. coli is catalyzed by RimJ and that fully acetylated Talpha1 can be obtained by co-expressing with RimJ.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    10 Oct 2026, 08:55 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 03:02 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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