An HPLC assay that uses fluorescently labeled DSIP(isoAsp) as substrate detected approximately 1 pmol of substrate and measured PIMT activity in HEK293 lysate.
In plain wordsIn lab tests, a new method that uses a labeled DSIP piece as bait measured an enzyme's activity in cell extracts without radioactivity.
- Outcome
- Not applicableDetection limit of the PIMT activity HPLC assay with NBD-DSIP(isoAsp) substrate
- Study
n: not reported in source
HPLC assay with cell lysates- Outcome group
- Receptor activity and mechanism · Pharmacology
Measurements
- limit of detection
- ≈ 1 pmol
- NBD-DSIP(isoAsp)
Source sentences
The limit of detection was estimated to be approximately 1 pmol of NBD-DSIP(isoAsp) (signal/noise ratio [S/N]=3)
1 context sentence(design, population, supporting numbers)
As a substrate, we used a fluorescently labeled delta sleep-inducing peptide (DSIP) that contains an isoaspartyl residue: 7-nitro-2,1,3-benzoxadiazole (NBD)-DSIP(isoAsp).
In this study, Delta sleep-inducing peptide (DSIP) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Delta sleep-inducing peptide (DSIP).