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Study · Animal study (in vivo) · rat · 2008

5 results

AMPK activation attenuates S6K1, 4E-BP1, and eEF2 signaling responses to high-frequency electrically stimulated skeletal muscle contractions.

Thomson DM, Fick CA, Gordon SE

Journal of applied physiology (Bethesda, Md. : 1985)10 Jan 2008Cited by 110 (Europe PMC)

PMID18187610 (opens in a new tab)DOI10.1152/japplphysiol.00915.2007 (opens in a new tab)PepBankPUB-18187610
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study5

5 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Mechanism

    In rats, AICAR increased post-stimulation AMPK Thr172 phosphorylation compared with saline.

    In plain wordsIn rats, AICAR increased muscle AMPK phosphorylation after electrical stimulation compared with saline.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseAMPK phosphorylation
    Study

    ratn: not reported in source

    electrically stimulated extensor digitorum longus muscle
    Comparator
    saline
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    1 mg/g body wt · subcutaneousAs given in this study (rat).

    Source sentences

    Summary
    AICAR treatment increased (P < or = 0.05) post-STIM AMPK (Thr172) and ACC phosphorylation (Ser79/221), inhibited post-STIM S6K1 (Thr389) and 4E-BP1 (gel shift) phosphorylation, and elevated post-STIM eEF2 phosphorylation (Thr56).
    2 context sentences
    Summary · context
    Extensor digitorum longus muscles (n = 8/group) were subjected to a 22-min bout of lengthening contractions by high-frequency sciatic nerve electrical stimulation (STIM) in young adult (8 mo) Fischer 344 x Brown Norway male rats.
    Summary · context
    Forty minutes before electrical stimulation, rats were subcutaneously injected with saline or 5-aminoimidazole-4-carboxamide-1-4-ribofuranoside (AICAR; 1 mg/g body wt), an AMPK activator.
  2. 02AnimalAnimal study (in vivo)Mechanism

    In rats, AICAR increased post-stimulation ACC Ser79/221 phosphorylation compared with saline.

    In plain wordsIn rats, AICAR increased muscle ACC phosphorylation after electrical stimulation compared with saline.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseACC phosphorylation
    Study

    ratn: not reported in source

    electrically stimulated extensor digitorum longus muscle
    Comparator
    saline
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    1 mg/g body wt · subcutaneousAs given in this study (rat).

    Source sentences

    Summary
    AICAR treatment increased (P < or = 0.05) post-STIM AMPK (Thr172) and ACC phosphorylation (Ser79/221), inhibited post-STIM S6K1 (Thr389) and 4E-BP1 (gel shift) phosphorylation, and elevated post-STIM eEF2 phosphorylation (Thr56).
    2 context sentences
    Summary · context
    Extensor digitorum longus muscles (n = 8/group) were subjected to a 22-min bout of lengthening contractions by high-frequency sciatic nerve electrical stimulation (STIM) in young adult (8 mo) Fischer 344 x Brown Norway male rats.
    Summary · context
    Forty minutes before electrical stimulation, rats were subcutaneously injected with saline or 5-aminoimidazole-4-carboxamide-1-4-ribofuranoside (AICAR; 1 mg/g body wt), an AMPK activator.
  3. 03AnimalAnimal study (in vivo)Mechanism

    In rats, AICAR inhibited post-stimulation S6K1 Thr389 phosphorylation compared with saline.

    In plain wordsIn rats, AICAR reduced muscle S6K1 phosphorylation after electrical stimulation compared with saline.

    Peptide
    AICAR (acadesine)
    Outcome
    DecreaseS6K1 phosphorylation
    Study

    ratn: not reported in source

    electrically stimulated extensor digitorum longus muscle
    Comparator
    saline
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    1 mg/g body wt · subcutaneousAs given in this study (rat).

    Source sentences

    Summary
    AICAR treatment increased (P < or = 0.05) post-STIM AMPK (Thr172) and ACC phosphorylation (Ser79/221), inhibited post-STIM S6K1 (Thr389) and 4E-BP1 (gel shift) phosphorylation, and elevated post-STIM eEF2 phosphorylation (Thr56).
    2 context sentences
    Summary · context
    Extensor digitorum longus muscles (n = 8/group) were subjected to a 22-min bout of lengthening contractions by high-frequency sciatic nerve electrical stimulation (STIM) in young adult (8 mo) Fischer 344 x Brown Norway male rats.
    Summary · context
    Forty minutes before electrical stimulation, rats were subcutaneously injected with saline or 5-aminoimidazole-4-carboxamide-1-4-ribofuranoside (AICAR; 1 mg/g body wt), an AMPK activator.
  4. 04AnimalAnimal study (in vivo)Mechanism

    In rats, AICAR inhibited post-stimulation 4E-BP1 phosphorylation compared with saline.

    In plain wordsIn rats, AICAR reduced muscle 4E-BP1 phosphorylation after electrical stimulation compared with saline.

    Peptide
    AICAR (acadesine)
    Outcome
    Decrease4E-BP1 phosphorylation
    Study

    ratn: not reported in source

    electrically stimulated extensor digitorum longus muscle
    Comparator
    saline
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    1 mg/g body wt · subcutaneousAs given in this study (rat).

    Source sentences

    Summary
    AICAR treatment increased (P < or = 0.05) post-STIM AMPK (Thr172) and ACC phosphorylation (Ser79/221), inhibited post-STIM S6K1 (Thr389) and 4E-BP1 (gel shift) phosphorylation, and elevated post-STIM eEF2 phosphorylation (Thr56).
    2 context sentences
    Summary · context
    Extensor digitorum longus muscles (n = 8/group) were subjected to a 22-min bout of lengthening contractions by high-frequency sciatic nerve electrical stimulation (STIM) in young adult (8 mo) Fischer 344 x Brown Norway male rats.
    Summary · context
    Forty minutes before electrical stimulation, rats were subcutaneously injected with saline or 5-aminoimidazole-4-carboxamide-1-4-ribofuranoside (AICAR; 1 mg/g body wt), an AMPK activator.
  5. 05AnimalAnimal study (in vivo)Mechanism

    In rats, AICAR elevated post-stimulation eEF2 Thr56 phosphorylation compared with saline.

    In plain wordsIn rats, AICAR increased muscle eEF2 phosphorylation after electrical stimulation compared with saline.

    Peptide
    AICAR (acadesine)
    Outcome
    IncreaseeEF2 phosphorylation
    Study

    ratn: not reported in source

    electrically stimulated extensor digitorum longus muscle
    Comparator
    saline
    Outcome group
    Receptor activity and mechanism · Musculoskeletal
    Studied dose
    1 mg/g body wt · subcutaneousAs given in this study (rat).

    Source sentences

    Summary
    AICAR treatment increased (P < or = 0.05) post-STIM AMPK (Thr172) and ACC phosphorylation (Ser79/221), inhibited post-STIM S6K1 (Thr389) and 4E-BP1 (gel shift) phosphorylation, and elevated post-STIM eEF2 phosphorylation (Thr56).
    2 context sentences
    Summary · context
    Extensor digitorum longus muscles (n = 8/group) were subjected to a 22-min bout of lengthening contractions by high-frequency sciatic nerve electrical stimulation (STIM) in young adult (8 mo) Fischer 344 x Brown Norway male rats.
    Summary · context
    Forty minutes before electrical stimulation, rats were subcutaneously injected with saline or 5-aminoimidazole-4-carboxamide-1-4-ribofuranoside (AICAR; 1 mg/g body wt), an AMPK activator.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    21 Oct 2026, 12:50 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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