Recombinant human MGF was expressed in Escherichia coli as inclusion bodies at about 100-200 mg/l, then purified and refolded.
In plain wordsIn bacteria, researchers made recombinant MGF as inclusion bodies at about 100-200 mg/l, then purified and refolded it.
- Peptide
- MGF / PEG-MGF
- Outcome
- Not applicableRecombinant MGF yield in E. coli
- Study
Escherichia colin: not reported in source
Recombinant expression in E. coli- Outcome group
- Synthesis and manufacturing · Pharmacology
Measurements
- Yield as inclusion bodies
- 100 to 200 mg/l
- E. coli expression
Source sentences
The recombinant MGF of directly examining--was expressed in Escherichia coli as inclusion bodies (about 100-200mg/l), purified and refolded.
1 context sentence(design, population, supporting numbers)
Here, we describe isolation and purification of recombinant MGF thus allowing for the first time the possibility of direct examining MGF effects.
In this study, MGF / PEG-MGF was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking MGF / PEG-MGF.