In glucotoxic islet and beta-cell experiments, AICAR eliminated the beneficial effect of stevioside on glucose-stimulated insulin secretion.
In plain wordsIn lab tests, AICAR removed stevioside's insulin secretion benefit compared with stevioside without AICAR.
- Peptide
- AICAR (acadesine)
- Outcome
- DecreaseStevioside benefit on glucose-stimulated insulin secretion
- Study
Mouse (isolated islets); species of INS-1E cells not reportedn: not reported in source
Glucotoxicity in isolated islets and INS-1E cells- Comparator
- Stevioside without AICAR
- Outcome group
- Glycemic control · Endocrine and hormones
- Studied dose
- 10(-4) MAs given in this study (Mouse (isolated islets); species of INS-1E cells not reported).
Source sentences
The ACC dephosphorylation inhibitor okadaic acid (OKA, 10(-8) M), and 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranoside (AICAR, 10(-4) M), an activator of 5'-AMP protein kinase that phosphorylates ACC, eliminated the beneficial effect of SVS.
2 context sentences(design, population, supporting numbers)
We found that 48-h exposure to high glucose impairs GSIS from mouse islets and INS-1E cells, an effect that is partly counteracted by SVS.
To investigate this, we exposed isolated mouse islets as well as clonal INS-1E beta-cells for 48 h to 27 or 16.7 mM glucose, respectively.