Differential regulation of GHRH-receptor and GHS-receptor expression by long-term in vitro treatment of ovine pituitary cells with GHRP-2 and GHRH.

Roh SG, Doconto M, Feng DD, Chen C

Endocrine1 Aug 2006Cited by 12 (Europe PMC)

PMID17185792DOI10.1385/endo:30:1:55PepBankPUB-17185792

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study10

2 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In ovine somatotropes, high-dose GHRP‑2 decreased GHRH-R mRNA levels.
In plain wordsIn sheep pituitary cells, a high dose of GHRP‑2 lowered GHRH receptor messenger RNA.
Peptide
GHRP-2
Outcome
DecreaseGHRH-R mRNA with high-dose GHRP-2
Study
ovinen: not reported in source
Primary cultured ovine somatotropes
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
High dose (10(-7) M)As given in this study (ovine).
Source sentences
Summary
High doses of GHRP-2 treatment decreased the levels of both GHRH-R and GHS-R mRNA.
1 context sentence(design, population, supporting numbers)
Summary · context
To examine the long-term effect of GHRH and/or GHRP on somatotropes, primary cultured ovine somatotropes were treated with GHRH (10(-9) and 10(-8) M) and GHRP-2 (10(-8) and 10(-7) M) for up to 2 d.
Report a problemFND-1718579206
02LabIn vitro (cells)Mechanism
In ovine somatotropes, high-dose GHRP‑2 decreased GHS-R mRNA levels.
In plain wordsIn sheep pituitary cells, a high dose of GHRP‑2 lowered ghrelin receptor messenger RNA.
Peptide
GHRP-2
Outcome
DecreaseGHS-R mRNA with high-dose GHRP-2
Study
ovinen: not reported in source
Primary cultured ovine somatotropes
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
High dose (10(-7) M)As given in this study (ovine).
Source sentences
Summary
High doses of GHRP-2 treatment decreased the levels of both GHRH-R and GHS-R mRNA.
1 context sentence(design, population, supporting numbers)
Summary · context
To examine the long-term effect of GHRH and/or GHRP on somatotropes, primary cultured ovine somatotropes were treated with GHRH (10(-9) and 10(-8) M) and GHRP-2 (10(-8) and 10(-7) M) for up to 2 d.
Report a problemFND-1718579207

Studies that did not test the peptide alone8

In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-2.

03LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In ovine somatotropes, 24 h treatment with low doses of GHRH plus GHRP‑2 increased accumulated GH secretion more than high doses of the pair.
In plain wordsIn sheep pituitary cells, low doses of GHRH and GHRP‑2 together released more GH over 24 hours than high doses.
Peptide
GHRP-2
Outcome
IncreaseAccumulated GH secretion with combined low doses of GHRH and GHRP-2
Study
ovinen: not reported in source
Primary cultured ovine somatotropes
Comparator
High doses of GHRH and GHRP-2
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Combined low doses of GHRH (10(-9) M) and GHRP-2 (10(-8) M) treatment for 24 h increased accumulated GH secretion, significantly more than that induced by high doses of GHRH (10(-8) M) and GHRP-2 (10(-7) M).
1 context sentence(design, population, supporting numbers)
Summary · context
To examine the long-term effect of GHRH and/or GHRP on somatotropes, primary cultured ovine somatotropes were treated with GHRH (10(-9) and 10(-8) M) and GHRP-2 (10(-8) and 10(-7) M) for up to 2 d.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1718579201
04LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In ovine somatotropes, 24 h treatment with low doses of GHRH and GHRP‑2 increased GHRH-R mRNA levels.
In plain wordsIn sheep pituitary cells, low doses of GHRH and GHRP‑2 together raised GHRH receptor messenger RNA after 24 hours.
Peptide
GHRP-2
Outcome
IncreaseGHRH-R mRNA after 24 h of low-dose GHRH and GHRP-2
Study
ovinen: not reported in source
Primary cultured ovine somatotropes
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
While levels of GHRH-R mRNA increased, GHS-R mRNA levels were decreased by low doses of GHRH and GHRP-2 for 24 h.
1 context sentence(design, population, supporting numbers)
Summary · context
To examine the long-term effect of GHRH and/or GHRP on somatotropes, primary cultured ovine somatotropes were treated with GHRH (10(-9) and 10(-8) M) and GHRP-2 (10(-8) and 10(-7) M) for up to 2 d.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1718579202
05LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In ovine somatotropes, 24 h treatment with low doses of GHRH and GHRP‑2 decreased GHS-R mRNA levels.
In plain wordsIn sheep pituitary cells, low doses of GHRH and GHRP‑2 together lowered ghrelin receptor messenger RNA after 24 hours.
Peptide
GHRP-2
Outcome
DecreaseGHS-R mRNA after 24 h of low-dose GHRH and GHRP-2
Study
ovinen: not reported in source
Primary cultured ovine somatotropes
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
While levels of GHRH-R mRNA increased, GHS-R mRNA levels were decreased by low doses of GHRH and GHRP-2 for 24 h.
1 context sentence(design, population, supporting numbers)
Summary · context
To examine the long-term effect of GHRH and/or GHRP on somatotropes, primary cultured ovine somatotropes were treated with GHRH (10(-9) and 10(-8) M) and GHRP-2 (10(-8) and 10(-7) M) for up to 2 d.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1718579203
06LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In ovine somatotropes, 2 d of high doses of GHRH and/or GHRP‑2 did not increase GH secretion on the second day.
In plain wordsIn sheep pituitary cells, two days of high doses of GHRH and/or GHRP‑2 did not raise GH release on the second day.
Peptide
GHRP-2
Outcome
No significant changeGH secretion on day 2 with high doses of GHRH and/or GHRP-2
Study
ovinen: not reported in source
Primary cultured ovine somatotropes
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
High doses of GHRH and/or GHRP-2 for 2 d did not increase GH secretion in the second day of treatment and reduced the level of GHRH-R mRNA.
1 context sentence(design, population, supporting numbers)
Summary · context
To examine the long-term effect of GHRH and/or GHRP on somatotropes, primary cultured ovine somatotropes were treated with GHRH (10(-9) and 10(-8) M) and GHRP-2 (10(-8) and 10(-7) M) for up to 2 d.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1718579204
07LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In ovine somatotropes, 2 d of high doses of GHRH and/or GHRP‑2 reduced GHRH-R mRNA levels.
In plain wordsIn sheep pituitary cells, two days of high doses of GHRH and/or GHRP‑2 lowered GHRH receptor messenger RNA.
Peptide
GHRP-2
Outcome
DecreaseGHRH-R mRNA after 2 d of high doses of GHRH and/or GHRP-2
Study
ovinen: not reported in source
Primary cultured ovine somatotropes
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
High doses of GHRH and/or GHRP-2 for 2 d did not increase GH secretion in the second day of treatment and reduced the level of GHRH-R mRNA.
1 context sentence(design, population, supporting numbers)
Summary · context
To examine the long-term effect of GHRH and/or GHRP on somatotropes, primary cultured ovine somatotropes were treated with GHRH (10(-9) and 10(-8) M) and GHRP-2 (10(-8) and 10(-7) M) for up to 2 d.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1718579205
08LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In ovine somatotropes, 2 d of low doses of GHRH and/or GHRP‑2 increased GHS-R mRNA levels.
In plain wordsIn sheep pituitary cells, two days of low doses of GHRH and/or GHRP‑2 raised ghrelin receptor messenger RNA.
Peptide
GHRP-2
Outcome
IncreaseGHS-R mRNA after 2 d of low doses of GHRH and/or GHRP-2
Study
ovinen: not reported in source
Primary cultured ovine somatotropes
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Low doses of GHRH and/or GHRP-2 for 2 d increased the level of GHS-R mRNA without changing GHRH-R mRNA levels.
1 context sentence(design, population, supporting numbers)
Summary · context
To examine the long-term effect of GHRH and/or GHRP on somatotropes, primary cultured ovine somatotropes were treated with GHRH (10(-9) and 10(-8) M) and GHRP-2 (10(-8) and 10(-7) M) for up to 2 d.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1718579208
09LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In ovine somatotropes, 2 d of low doses of GHRH and/or GHRP‑2 did not change GHRH-R mRNA levels.
In plain wordsIn sheep pituitary cells, two days of low doses of GHRH and/or GHRP‑2 did not change GHRH receptor messenger RNA.
Peptide
GHRP-2
Outcome
No significant changeGHRH-R mRNA after 2 d of low doses of GHRH and/or GHRP-2
Study
ovinen: not reported in source
Primary cultured ovine somatotropes
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Low doses of GHRH and/or GHRP-2 for 2 d increased the level of GHS-R mRNA without changing GHRH-R mRNA levels.
1 context sentence(design, population, supporting numbers)
Summary · context
To examine the long-term effect of GHRH and/or GHRP on somatotropes, primary cultured ovine somatotropes were treated with GHRH (10(-9) and 10(-8) M) and GHRP-2 (10(-8) and 10(-7) M) for up to 2 d.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1718579209
10LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In ovine somatotropes, 2 d of low doses of GHRH and/or GHRP‑2 increased ghrelin- or ghrelin/GHRH-induced GH secretion.
In plain wordsIn sheep pituitary cells, two days of low doses of GHRH and/or GHRP‑2 made the cells release more GH when ghrelin was added.
Peptide
GHRP-2
Outcome
IncreaseGhrelin- or ghrelin/GHRH-induced GH secretion after low-dose pretreatment
Study
ovinen: not reported in source
Primary cultured ovine somatotropes
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Source sentences
Summary
Low doses of GHRH and/or GHRP-2 for 2 d increased the level of GHS-R mRNA without changing GHRH-R mRNA levels. Such treatment also increased ghrelin- (10(-9) M) or ghrelin/GHRH (10(-9) M)-induced GH secretion.
1 context sentence(design, population, supporting numbers)
Summary · context
To examine the long-term effect of GHRH and/or GHRP on somatotropes, primary cultured ovine somatotropes were treated with GHRH (10(-9) and 10(-8) M) and GHRP-2 (10(-8) and 10(-7) M) for up to 2 d.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1718579210

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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Differential regulation of GHRH-receptor and GHS-receptor expression by long-term in vitro treatment of ovine pituitary cells with GHRP-2 and GHRH.

Summary

Results from this study10

Studies that did not test the peptide alone8

Version history

Subjects

Publication types

MeSH terms