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Study · In vitro (cells) · 2006

6 results

LONG R3IGF-I as a more potent alternative to insulin in serum-free culture of HEK293 cells.

Voorhamme D, Yandell CA

Molecular biotechnology1 Oct 2006Cited by 9 (Europe PMC)

PMID17172665 (opens in a new tab)DOI10.1385/mb:34:2:201 (opens in a new tab)PepBankPUB-17172665
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In serum-free cultures of HEK293 cells, LONG R3IGF-I acted as a more potent growth and survival factor than either insulin or native IGF-I.

    In plain wordsIn cells, LONG R3IGF-I supported the growth and survival of kidney cells better than insulin or native IGF-I.

    Peptide
    IGF-1 LR3
    Outcome
    IncreaseGrowth and survival of HEK293 cells in serum-free culture
    Study

    humann: not reported in source

    HEK293 cells in serum-free culture
    Comparator
    Insulin or native IGF-I
    Outcome group
    Cell proliferation and differentiation · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    LONG R3IGF-I also acts as a more potent growth and survival factor than either insulin or native IGF-I in SF culture of human embryonic kidney (HEK293) cells.
    1 context sentence
    Summary · context
    To investigate the basis of the enhanced potency of LONG R3IGF-I we have examined the mechanism of action of these mitogens in HEK293 cells.
  2. 02LabIn vitro (cells)Mechanism

    In HEK293 cells, LONG R3IGF-I activated the Type I IGF receptor in a dose-responsive manner, as did insulin and IGF-I.

    In plain wordsIn cells, LONG R3IGF-I switched on the Type I IGF receptor more strongly as the dose went up.

    Peptide
    IGF-1 LR3
    Outcome
    IncreaseType I IGF receptor activation
    Study

    humann: not reported in source

    HEK293 cells in serum-free culture
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    All mitogens tested were found to activate the Type I IGF receptor (IGF-IR) and insulin receptor (IR) in a dose-responsive manner.
    1 context sentence
    Summary · context
    To investigate the basis of the enhanced potency of LONG R3IGF-I we have examined the mechanism of action of these mitogens in HEK293 cells.
  3. 03LabIn vitro (cells)Mechanism

    In HEK293 cells, LONG R3IGF-I activated the insulin receptor in a dose-responsive manner, as did insulin and IGF-I.

    In plain wordsIn cells, LONG R3IGF-I switched on the insulin receptor more strongly as the dose went up.

    Peptide
    IGF-1 LR3
    Outcome
    IncreaseInsulin receptor activation
    Study

    humann: not reported in source

    HEK293 cells in serum-free culture
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    All mitogens tested were found to activate the Type I IGF receptor (IGF-IR) and insulin receptor (IR) in a dose-responsive manner.
    1 context sentence
    Summary · context
    To investigate the basis of the enhanced potency of LONG R3IGF-I we have examined the mechanism of action of these mitogens in HEK293 cells.
  4. 04LabIn vitro (cells)Mechanism

    In HEK293 cells, at lower concentrations LONG R3IGF-I activated the Type I IGF receptor more than insulin or IGF-I did.

    In plain wordsIn cells, at low doses LONG R3IGF-I switched on the IGF receptor more than insulin or IGF-I.

    Peptide
    IGF-1 LR3
    Outcome
    IncreaseType I IGF receptor activation at lower concentrations
    Study

    humann: not reported in source

    HEK293 cells in serum-free culture
    Comparator
    Insulin or IGF-I
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    However, the level of activation of both receptors after stimulation with LONG R3IGF-I, at lower concentrations, was greater than with either insulin or IGF-I.
    1 context sentence
    Summary · context
    To investigate the basis of the enhanced potency of LONG R3IGF-I we have examined the mechanism of action of these mitogens in HEK293 cells.
  5. 05LabIn vitro (cells)Mechanism

    In HEK293 cells, at lower concentrations LONG R3IGF-I activated the insulin receptor more than insulin or IGF-I did.

    In plain wordsIn cells, at low doses LONG R3IGF-I switched on the insulin receptor more than insulin or IGF-I.

    Peptide
    IGF-1 LR3
    Outcome
    IncreaseInsulin receptor activation at lower concentrations
    Study

    humann: not reported in source

    HEK293 cells in serum-free culture
    Comparator
    Insulin or IGF-I
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    However, the level of activation of both receptors after stimulation with LONG R3IGF-I, at lower concentrations, was greater than with either insulin or IGF-I.
    1 context sentence
    Summary · context
    To investigate the basis of the enhanced potency of LONG R3IGF-I we have examined the mechanism of action of these mitogens in HEK293 cells.
  6. 06LabIn vitro (cells)Mechanism

    In HEK293 cells, Type I IGF receptor activation fell at higher concentrations of LONG R3IGF-I, which suggests a bell-shaped dose-response curve.

    In plain wordsIn cells, LONG R3IGF-I switched on the IGF receptor less at high doses, which suggests a bell-shaped dose curve.

    Peptide
    IGF-1 LR3
    Outcome
    DecreaseType I IGF receptor activation at higher concentrations
    Study

    humann: not reported in source

    HEK293 cells in serum-free culture
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Interestingly, the decrease in IGF-IR activation at higher concentrations of LONG R3IGF-I suggests that the dose-response curve may be bell-shaped.
    1 context sentence
    Summary · context
    To investigate the basis of the enhanced potency of LONG R3IGF-I we have examined the mechanism of action of these mitogens in HEK293 cells.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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