In SCN slices from mice, the proportion of rhythmic cells fell with increasing VIP deficiency, from wild-type through heterozygous to VIP/PHI-null mice, with a parallel trend to lower amplitude in remaining rhythmic cells.
In plain wordsIn mouse brain clock slices, fewer cells kept a daily rhythm the more VIP was missing.
- Outcome
- DecreaseProportion of rhythmic SCN cells by degree of VIP deficiency
- Study
mousen: not reported in source
SCN brain slices from VIP/PHI-deficient mice- Comparator
- Wild-type (VIP/PHI+/+)
- Outcome group
- Circadian rhythm · Nervous system and cognition
Source sentences
We found decreasing proportions of rhythmic cells in SCN slices from VIP/PHI(+/+) ( approximately 91%, n = 23) through VIP/PHI(-/+) ( approximately 71%, n = 28) to VIP/PHI(-/-) mice (62%; n = 37) and a parallel trend toward decreasing amplitude in the remaining rhythmic cells.
1 context sentence(design, population, supporting numbers)
To investigate this, we used suction electrodes to extracellularly record multiple- and single-unit electrical activity in SCN brain slices from mice with varying degrees of VIP deficiency, ranging from wild-type (VIP/PHI(+/+)) to heterozygous (VIP/PHI(+/-)) and VIP/PHI(-/-) animals.
In this study, Vasoactive intestinal peptide (VIP, aviptadil) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Vasoactive intestinal peptide (VIP, aviptadil).