Bovine and human cathelicidin cationic host defense peptides similarly suppress transcriptional responses to bacterial lipopolysaccharide.

Mookherjee N, Wilson HL, Doria S, Popowych Y, Falsafi R, Yu JJ, Li Y, Veatch S, Roche FM, Brown KL, Brinkman FS, Hokamp K and 4 more

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Potter A, Babiuk LA, Griebel PJ, Hancock RE

Journal of leukocyte biology30 Aug 2006Cited by 84 (Europe PMC)

PMID16943385DOI10.1189/jlb.0106048PepBankPUB-16943385

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Activity measurement
In human monocytes, LL‑37 at 5 µg/ml suppressed 106 of the 125 genes up-regulated by 10 ng/ml LPS.
In plain wordsIn human immune cells, LL‑37 switched down most genes that bacterial LPS had switched on.
Peptide
LL-37
Outcome
DecreaseLPS-induced gene up-regulation (microarray)
Study
humann: not reported in source
Human monocytic cells
Comparator
LPS alone
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
5 µg/mlAs given in this study (human).
Measurements
Genes up-regulated by LPS
125 genes
human monocytes, 10 ng/ml LPS
Up-regulated genes suppressed by LL-37
106 genes
5 µg/ml LL-37
Source sentences
Summary
Microarray studies indicated that 10 ng/ml LPS led to the up-regulation of 125 genes in human monocytes, 106 of which were suppressed in the presence of 5 mug/ml of the human peptide LL-37.
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, temporal transcriptional expression profiles were analyzed in human and bovine monocytic cells in response to the TLR-4 agonist, LPS, in the presence or absence of their respective host defense peptides.
Summary · context
The cathelicidin peptides, human LL-37 and bovine myeloid antimicrobial peptide-27 (BMAP-27), are homologs, yet they have diverged notably in terms of sequence similarity.
Report a problemFND-1694338501
02LabIn vitro (cells)Activity measurement
In human monocytic cells, LL‑37 profoundly suppressed many of 20 LPS-induced genes measured by real-time PCR.
In plain wordsIn human immune cells, LL‑37 strongly reduced the activity of many genes that LPS had switched on.
Peptide
LL-37
Outcome
DecreaseLPS-induced gene expression (qPCR, 20 genes)
Study
humann: not reported in source
Human monocytic cells
Comparator
LPS alone
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
Dose not stated
Measurements
LPS-induced genes analysed
20 genes
bovine and human monocytic cells
Source sentences
Summary
The transcriptional trends of 20 LPS-induced genes were analyzed in bovine and human monocytic cells. These studies demonstrated conserved trends of gene responses in that both peptides were able to profoundly suppress many LPS-induced genes.
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, temporal transcriptional expression profiles were analyzed in human and bovine monocytic cells in response to the TLR-4 agonist, LPS, in the presence or absence of their respective host defense peptides.
Summary · context
The cathelicidin peptides, human LL-37 and bovine myeloid antimicrobial peptide-27 (BMAP-27), are homologs, yet they have diverged notably in terms of sequence similarity.
Report a problemFND-1694338502
03LabIn vitro (cells)Activity measurement
In monocytic cells, LL‑37 showed potent antiendotoxin activity at low, physiologically relevant concentrations.
In plain wordsIn immune cells, LL‑37 blocked the effects of bacterial endotoxin at low concentrations.
Peptide
LL-37
Outcome
DecreaseAntiendotoxin activity
Study
humann: not reported in source
Human monocytic cells
Comparator
LPS alone
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
low, physiologically relevant concentrationsAs given in this study (human).
Source sentences
Summary
In spite of their low sequence similarities, both of these cathelicidin peptides demonstrated potent, antiendotoxin activity in monocytic cells at low, physiologically relevant concentrations.
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, temporal transcriptional expression profiles were analyzed in human and bovine monocytic cells in response to the TLR-4 agonist, LPS, in the presence or absence of their respective host defense peptides.
Summary · context
The cathelicidin peptides, human LL-37 and bovine myeloid antimicrobial peptide-27 (BMAP-27), are homologs, yet they have diverged notably in terms of sequence similarity.
Report a problemFND-1694338503
04LabIn vitro (cells)Mechanism
In monocytic cells, LL‑37 suppressed LPS-induced translocation of NF-kappaB subunit p50 into the nucleus.
In plain wordsIn immune cells, LL‑37 reduced LPS-triggered movement of the NF-kappaB p50 protein into the nucleus.
Peptide
LL-37
Outcome
DecreaseLPS-induced nuclear translocation of NF-kappaB p50
Study
humann: not reported in source
Human monocytic cells
Comparator
LPS alone
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
Dose not stated
Source sentences
Summary
Consistent with this, the human and bovine peptides suppressed LPS-induced translocation of NF-kappaB subunits p50 and p65 into the nucleus of monocytic cells.
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, temporal transcriptional expression profiles were analyzed in human and bovine monocytic cells in response to the TLR-4 agonist, LPS, in the presence or absence of their respective host defense peptides.
Summary · context
The cathelicidin peptides, human LL-37 and bovine myeloid antimicrobial peptide-27 (BMAP-27), are homologs, yet they have diverged notably in terms of sequence similarity.
Report a problemFND-1694338504
05LabIn vitro (cells)Mechanism
In monocytic cells, LL‑37 suppressed LPS-induced translocation of NF-kappaB subunit p65 into the nucleus.
In plain wordsIn immune cells, LL‑37 reduced LPS-triggered movement of the NF-kappaB p65 protein into the nucleus.
Peptide
LL-37
Outcome
DecreaseLPS-induced nuclear translocation of NF-kappaB p65
Study
humann: not reported in source
Human monocytic cells
Comparator
LPS alone
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
Dose not stated
Source sentences
Summary
Consistent with this, the human and bovine peptides suppressed LPS-induced translocation of NF-kappaB subunits p50 and p65 into the nucleus of monocytic cells.
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, temporal transcriptional expression profiles were analyzed in human and bovine monocytic cells in response to the TLR-4 agonist, LPS, in the presence or absence of their respective host defense peptides.
Summary · context
The cathelicidin peptides, human LL-37 and bovine myeloid antimicrobial peptide-27 (BMAP-27), are homologs, yet they have diverged notably in terms of sequence similarity.
Report a problemFND-1694338505
06LabIn vitro (cells)Activity measurement
In monocytic cells, LL‑37 alone at 5 µg/ml tended to influence expression of selected genes to a lesser extent than the same amount of BMAP-27.
In plain wordsIn immune cells, LL‑37 alone tended to change selected genes less than the same amount of BMAP-27.
Peptide
LL-37
Outcome
Not applicableGene expression change by peptide alone versus BMAP-27
Study
humann: not reported in source
Human monocytic cells
Comparator
BMAP-27 5 µg/ml
Outcome group
Inflammation markers · Immune and inflammation
Studied dose
5 µg/mlAs given in this study (human).
Source sentences
Summary
treatment with 5 mug/ml BMAP-27 alone tended to influence gene expression (RELA, TNF-alpha-induced protein 2, MAPK phosphatase 1/dual specificity phosphatase 1, IkappaBkappaB, NFkappaBIL1, TNF receptor-associated factor 2) to a greater extent than did the same amount of human LL-37.
2 context sentences(design, population, supporting numbers)
Summary · context
In this study, temporal transcriptional expression profiles were analyzed in human and bovine monocytic cells in response to the TLR-4 agonist, LPS, in the presence or absence of their respective host defense peptides.
Summary · context
The cathelicidin peptides, human LL-37 and bovine myeloid antimicrobial peptide-27 (BMAP-27), are homologs, yet they have diverged notably in terms of sequence similarity.
Report a problemFND-1694338506

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 3Current
11 Oct 2026, 00:50 UTC
Study results updated
Version 2
9 Oct 2026, 03:03 UTC
Study data updated · source update from Europe PMC
Version 1
9 Oct 2026, 02:59 UTC
First added · source update from Europe PMC

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