Control of culture environment for improved polyethylenimine-mediated transient production of recombinant monoclonal antibodies by CHO cells.

Galbraith DJ, Tait AS, Racher AJ, Birch JR, James DC

Biotechnology progress1 May 2006Cited by 57 (Europe PMC)

PMID16739959DOI10.1021/bp050339vPepBankPUB-16739959

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

3 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Other
In CHO cell cultures, adding recombinant LR3-IGF increased monoclonal antibody product titer 2-fold.
In plain wordsIn cells, adding LR3-IGF to hamster cell cultures raised antibody yield 2-fold.
Peptide
IGF-1 LR3
Outcome
IncreaseMonoclonal antibody product titer with LR3-IGF alone
Study
Chinese hamstern: not reported in source
CHO cells, PEI-mediated transient transfection
Outcome group
Synthesis and manufacturing · Pharmacology
Studied dose
Dose not stated
Measurements
Product titer increase
2-fold
LR3-IGF alone
Source sentences
Summary
Separately, both addition of recombinant insulin-like growth factor (LR3-IGF) and a reduction in culture temperature to 32 degrees C were found to increase product titer 2- and 3-fold, respectively.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study we describe optimization of polyethylenimine (PEI)-mediated transient production of recombinant protein by CHO cells by facile manipulation of a chemically defined culture environment to limit accumulation of nonproductive cell biomass, increase the duration of recombinant protein prod…
Report a problemFND-1673995901
02LabIn vitro (cells)Other
In CHO cell cultures, LR3-IGF and mild hypothermia acted synergistically to increase product titer 11-fold.
In plain wordsIn cells, LR3-IGF and a cooler culture temperature raised antibody yield 11-fold.
Peptide
IGF-1 LR3
Outcome
IncreaseMonoclonal antibody product titer with LR3-IGF plus mild hypothermia
Study
Chinese hamstern: not reported in source
CHO cells, PEI-mediated transient transfection
Outcome group
Synthesis and manufacturing · Pharmacology
Studied dose
Dose not stated
Measurements
Product titer increase
11-fold
LR3-IGF plus 32 degrees C
Source sentences
Summary
However, mild hypothermia and LR3-IGF acted synergistically to increase product titer 11-fold.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study we describe optimization of polyethylenimine (PEI)-mediated transient production of recombinant protein by CHO cells by facile manipulation of a chemically defined culture environment to limit accumulation of nonproductive cell biomass, increase the duration of recombinant protein prod…
Report a problemFND-1673995902
03LabIn vitro (cells)Mechanism
In CHO cell cultures, the higher product titer with LR3-IGF alone was solely a consequence of increased culture duration.
In plain wordsIn cells, LR3-IGF raised antibody yield only because the cultures lasted longer.
Peptide
IGF-1 LR3
Outcome
IncreaseCulture duration with LR3-IGF alone
Study
Chinese hamstern: not reported in source
CHO cells, PEI-mediated transient transfection
Outcome group
Synthesis and manufacturing · Pharmacology
Studied dose
Dose not stated
Source sentences
Summary
Although increased product titer in the presence of LR3-IGF alone was solely a consequence of increased culture duration
1 context sentence(design, population, supporting numbers)
Summary · context
In this study we describe optimization of polyethylenimine (PEI)-mediated transient production of recombinant protein by CHO cells by facile manipulation of a chemically defined culture environment to limit accumulation of nonproductive cell biomass, increase the duration of recombinant protein prod…
Report a problemFND-1673995903

Studies that did not test the peptide alone4

In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking IGF-1 LR3.

04LabNot a test of IGF-1 LR3 aloneIn vitro (cells)Other
In CHO cultures kept at 32 degrees C with LR3-IGF, the integral of viable cell concentration increased 4-fold.
In plain wordsIn cells, cooler cultures with LR3-IGF had 4 times the integral of viable cell concentration.
Peptide
IGF-1 LR3
Outcome
IncreaseIntegral of viable cell concentration with LR3-IGF at 32 degrees C
Study
Chinese hamstern: not reported in source
CHO cells, PEI-mediated transient transfection
Outcome group
Synthesis and manufacturing · Pharmacology
Measurements
IVC increase
4-fold
32 degrees C with LR3-IGF
Source sentences
Summary
For cultures maintained at 32 degrees C in the presence of LR3-IGF, IVC and qMab were increased 4- and 2.5-fold, respectively.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study we describe optimization of polyethylenimine (PEI)-mediated transient production of recombinant protein by CHO cells by facile manipulation of a chemically defined culture environment to limit accumulation of nonproductive cell biomass, increase the duration of recombinant protein prod…
In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.
Report a problemFND-1673995904
05LabNot a test of IGF-1 LR3 aloneIn vitro (cells)Other
In CHO cultures kept at 32 degrees C with LR3-IGF, the cell-specific Mab production rate (qMab) increased 2.5-fold.
In plain wordsIn cells, cooler cultures with LR3-IGF made 2.5 times more antibody per cell.
Peptide
IGF-1 LR3
Outcome
IncreaseCell-specific Mab production rate with LR3-IGF at 32 degrees C
Study
Chinese hamstern: not reported in source
CHO cells, PEI-mediated transient transfection
Outcome group
Synthesis and manufacturing · Pharmacology
Measurements
qMab increase
2.5-fold
32 degrees C with LR3-IGF
Source sentences
Summary
For cultures maintained at 32 degrees C in the presence of LR3-IGF, IVC and qMab were increased 4- and 2.5-fold, respectively.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study we describe optimization of polyethylenimine (PEI)-mediated transient production of recombinant protein by CHO cells by facile manipulation of a chemically defined culture environment to limit accumulation of nonproductive cell biomass, increase the duration of recombinant protein prod…
In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.
Report a problemFND-1673995905
06LabNot a test of IGF-1 LR3 aloneIn vitro (cells)Other
In CHO cultures kept at 32 degrees C with LR3-IGF, periodic resuspension of transfected cells in fresh media gave a 3-fold increase in accumulated Mab titer, from about 13 to about 39 mg L(-1).
In plain wordsIn cells, moving transfected cells into fresh media from time to time raised antibody yield 3-fold.
Peptide
IGF-1 LR3
Outcome
IncreaseAccumulated Mab titer with periodic resuspension in fresh media
Study
Chinese hamstern: not reported in source
CHO cells, PEI-mediated transient transfection
Outcome group
Synthesis and manufacturing · Pharmacology
Measurements
Accumulated Mab titer increase
3-fold
periodic resuspension
Accumulated Mab titer start
≈ 13 mg L(-1)
periodic resuspension
Accumulated Mab titer end
≈ 39 mg L(-1)
periodic resuspension
Source sentences
Summary
To further increase product yield from transfected DNA, the duration of transgene expression in cell populations maintained at 32 degrees C in the presence of LR3-IGF was doubled by periodic resuspension of transfected cells in fresh media, leading to a 3-fold increase in accumulated Mab titer from…
1 context sentence(design, population, supporting numbers)
Summary · context
In this study we describe optimization of polyethylenimine (PEI)-mediated transient production of recombinant protein by CHO cells by facile manipulation of a chemically defined culture environment to limit accumulation of nonproductive cell biomass, increase the duration of recombinant protein prod…
In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.
Report a problemFND-1673995906
07LabNot a test of IGF-1 LR3 aloneIn vitro (cells)Other
In CHO cultures with LR3-IGF at 32 degrees C, Mab glycosylation at Asn297 stayed essentially constant and was similar to that of Mab from stably transfected GS-CHO cells.
In plain wordsIn cells, the antibody sugar pattern at Asn297 did not change and matched that of antibody from stably transfected cells.
Peptide
IGF-1 LR3
Outcome
No significant changeMab glycosylation at Asn297
Study
Chinese hamstern: not reported in source
CHO cells, PEI-mediated transient transfection
Comparator
Mab from stably transfected GS-CHO cells
Outcome group
Synthesis and manufacturing · Pharmacology
Source sentences
Summary
Under these conditions, Mab glycosylation at Asn297 remained essentially constant and similar to that of the same Mab produced by stably transfected GS-CHO cells.
1 context sentence(design, population, supporting numbers)
Summary · context
In this study we describe optimization of polyethylenimine (PEI)-mediated transient production of recombinant protein by CHO cells by facile manipulation of a chemically defined culture environment to limit accumulation of nonproductive cell biomass, increase the duration of recombinant protein prod…
In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.
Report a problemFND-1673995907

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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