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Study · Animal study (in vivo) · rat · 2005

7 results

Growth hormone-releasing peptide-6 increases insulin-like growth factor-I mRNA levels and activates Akt in RCA-6 cells as a model of neuropeptide Y neurones.

Frago LM, Pañeda C, Argente J, Chowen JA

Journal of neuroendocrinology1 Nov 2005Cited by 16 (Europe PMC)

PMID16218998 (opens in a new tab)DOI10.1111/j.1365-2826.2005.01347.x (opens in a new tab)PepBankPUB-16218998
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

4 results from studies that gave the peptide. No result from this study comes from people.

  1. 01AnimalAnimal study (in vivo)Mechanism

    In rats, GHRP‑6 increased arcuate pAkt immunoreactivity, including in a subset of NPY-positive neurones.

    In plain wordsIn rats, GHRP‑6 increased activated Akt staining in the arcuate nucleus compared with controls.

    Peptide
    GHRP-6
    Outcome
    IncreaseArcuate pAkt immunoreactivity
    Study

    ratn: not reported in source

    Rat arcuate nucleus
    Comparator
    Control rats
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    In control rats, immunoreactive pAkt was practically undetectable; however, GHRP-6 increased p-Akt immunoreactivity in the arcuate nucleus, with a subset of neurones also being immunoreactive for NPY.
  2. 02LabIn vitro (cells)Mechanism

    In RCA-6 cells, GHRP‑6 stimulated NPY mRNA synthesis.

    In plain wordsIn cells from rat hypothalamus, GHRP‑6 increased NPY gene expression.

    Peptide
    GHRP-6
    Outcome
    IncreaseNPY mRNA synthesis
    Study

    ratn: not reported in source

    RCA-6 embryonic rat hypothalamic neuronal cells
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We found that GHRP-6 stimulates NPY and IGF-I mRNA synthesis and activates Akt in this cell line.
    1 context sentence
    Summary · context
    To determine if activation of this intracellular pathway is involved in modulation of NPY synthesis RCA-6 cells, an embryonic rat hypothalamic neuronal cell line that expresses NPY was used.
  3. 03LabIn vitro (cells)Mechanism

    In RCA-6 cells, GHRP‑6 stimulated IGF-I mRNA synthesis.

    In plain wordsIn cells from rat hypothalamus, GHRP‑6 increased IGF-I gene expression.

    Peptide
    GHRP-6
    Outcome
    IncreaseIGF-I mRNA synthesis
    Study

    ratn: not reported in source

    RCA-6 embryonic rat hypothalamic neuronal cells
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We found that GHRP-6 stimulates NPY and IGF-I mRNA synthesis and activates Akt in this cell line.
    1 context sentence
    Summary · context
    To determine if activation of this intracellular pathway is involved in modulation of NPY synthesis RCA-6 cells, an embryonic rat hypothalamic neuronal cell line that expresses NPY was used.
  4. 04LabIn vitro (cells)Mechanism

    In RCA-6 cells, GHRP‑6 activated Akt.

    In plain wordsIn cells from rat hypothalamus, GHRP‑6 activated Akt.

    Peptide
    GHRP-6
    Outcome
    IncreaseAkt activation
    Study

    ratn: not reported in source

    RCA-6 embryonic rat hypothalamic neuronal cells
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition
    Studied dose
    Dose not stated

    Source sentences

    Summary
    We found that GHRP-6 stimulates NPY and IGF-I mRNA synthesis and activates Akt in this cell line.
    1 context sentence
    Summary · context
    To determine if activation of this intracellular pathway is involved in modulation of NPY synthesis RCA-6 cells, an embryonic rat hypothalamic neuronal cell line that expresses NPY was used.

Studies that did not test the peptide alone3

In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-6.

  1. 05AnimalNot a test of GHRP-6 aloneAnimal study (in vivo)Mechanism

    In rats, NPY neurones showed IGF-I immunoreactivity in both control and GHRP‑6 experimental groups.

    In plain wordsIn rats, NPY nerve cells showed IGF-I staining in both experimental groups.

    Peptide
    GHRP-6
    Outcome
    Not applicableIGF-I immunoreactivity
    Study

    ratn: not reported in source

    Rat NPY neurones
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition

    Source sentences

    Summary
    Immunoreactivity for IGF-I was detected in NPY neurones in both experimental groups.
    1 context sentence
    Summary · context
    In control rats, immunoreactive pAkt was practically undetectable; however, GHRP-6 increased p-Akt immunoreactivity in the arcuate nucleus, with a subset of neurones also being immunoreactive for NPY.

    In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.

  2. 06LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism

    In RCA-6 cells, LY294002 inhibition of Akt did not inhibit GHRP‑6-induced NPY synthesis.

    In plain wordsIn rat hypothalamic cells, Akt inhibition did not reduce GHRP‑6-induced NPY synthesis compared with GHRP‑6 alone.

    Peptide
    GHRP-6
    Outcome
    No significant changeNPY synthesis during Akt inhibition
    Study

    ratn: not reported in source

    RCA-6 embryonic rat hypothalamic neuronal cells
    Comparator
    GHRP-6 without LY294002
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition

    Source sentences

    Summary
    Furthermore, inhibition of Akt activation by LY294002 treatment did not inhibit GHRP-6 induction of NPY or IGF-I synthesis.

    In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.

  3. 07LabNot a test of GHRP-6 aloneIn vitro (cells)Mechanism

    In RCA-6 cells, LY294002 inhibition of Akt did not inhibit GHRP‑6-induced IGF-I synthesis.

    In plain wordsIn rat hypothalamic cells, Akt inhibition did not reduce GHRP‑6-induced IGF-I synthesis compared with GHRP‑6 alone.

    Peptide
    GHRP-6
    Outcome
    No significant changeIGF-I synthesis during Akt inhibition
    Study

    ratn: not reported in source

    RCA-6 embryonic rat hypothalamic neuronal cells
    Comparator
    GHRP-6 without LY294002
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition

    Source sentences

    Summary
    Furthermore, inhibition of Akt activation by LY294002 treatment did not inhibit GHRP-6 induction of NPY or IGF-I synthesis.

    In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    21 Oct 2026, 15:58 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:13 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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