Activity-dependent neurotrophic factor-9 and NAP promote neurite outgrowth in rat hippocampal and cortical cultures.

Smith-Swintosky VL, Gozes I, Brenneman DE, D'Andrea MR, Plata-Salaman CR

Journal of molecular neuroscience : MN1 Jan 2005Cited by 52 (Europe PMC)

PMID15800376DOI10.1385/jmn:25:3:225PepBankPUB-15800376

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Efficacy outcome
In rat neuronal cultures, NAP promoted concentration-dependent neurite outgrowth, with maximal activity at femtomolar concentrations.
In plain wordsIn cells from rats, NAP increased nerve extension growth with concentration, with maximal activity at femtomolar concentrations.
Peptide
Davunetide
Outcome
IncreaseConcentration-dependent neurite outgrowth
Study
ratn: not reported in source
Rat hippocampal and cortical cultures
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Femtomolar concentrations at maximal activityAs given in this study (rat).
Source sentences
Summary
Using MAP2-FITC immunofluorescent labeling, we found that ADNF-9 and NAP promoted neurite outgrowth in a concentration-dependent manner, with maximal activity observed at femtomolar concentrations.
1 context sentence(design, population, supporting numbers)
Summary · context
The purpose of the present studies was to determine if ADNF-9 or NAP affects neurite outgrowth and synaptogenesis in rat hippocampal and cortical cultures.
Report a problemFND-1580037601
02LabIn vitro (cells)Efficacy outcome
In rat hippocampal cells, NAP stimulated outgrowth to approximately 150% of control.
In plain wordsIn cells from rat hippocampus, NAP stimulated nerve extension growth to approximately 150% of control.
Peptide
Davunetide
Outcome
IncreaseHippocampal neurite outgrowth
Study
ratn: not reported in source
Rat hippocampal cultures
Comparator
Control
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated
Measurements
Outgrowth
≈ 150 % of control
Hippocampal cells
Source sentences
Summary
Both peptides stimulated robust outgrowth in hippocampal cells (approximately 150% of control; p < 0.01) with a modest effect on cortical cells (approximately 20% of control; p < 0.05) similar to other known growth factors.
1 context sentence(design, population, supporting numbers)
Summary · context
The purpose of the present studies was to determine if ADNF-9 or NAP affects neurite outgrowth and synaptogenesis in rat hippocampal and cortical cultures.
Report a problemFND-1580037602
03LabIn vitro (cells)Efficacy outcome
In rat cortical cells, NAP had a modest outgrowth effect reported as approximately 20% of control.
In plain wordsIn cells from rat cortex, NAP had a modest growth effect reported as approximately 20% of control.
Peptide
Davunetide
Outcome
IncreaseCortical neurite outgrowth
Study
ratn: not reported in source
Rat cortical cultures
Comparator
Control
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated
Measurements
Outgrowth
≈ 20 % of control
Cortical cells
Source sentences
Summary
Both peptides stimulated robust outgrowth in hippocampal cells (approximately 150% of control; p < 0.01) with a modest effect on cortical cells (approximately 20% of control; p < 0.05) similar to other known growth factors.
1 context sentence(design, population, supporting numbers)
Summary · context
The purpose of the present studies was to determine if ADNF-9 or NAP affects neurite outgrowth and synaptogenesis in rat hippocampal and cortical cultures.
Report a problemFND-1580037603
04LabIn vitro (cells)Efficacy outcome
In rat neuronal cultures, the NAP outgrowth-promoting effect was abolished without serum.
In plain wordsIn cells from rats, NAP lost its nerve extension growth effect without serum.
Peptide
Davunetide
Outcome
No significant changeOutgrowth effect without serum
Study
ratn: not reported in source
Rat hippocampal and cortical cultures without serum
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
However, the outgrowth-promoting effect was abolished in the absence of serum, suggesting that soluble factors might be necessary for the neurotrophic activity.
1 context sentence(design, population, supporting numbers)
Summary · context
The purpose of the present studies was to determine if ADNF-9 or NAP affects neurite outgrowth and synaptogenesis in rat hippocampal and cortical cultures.
Report a problemFND-1580037604
05LabIn vitro (cells)Efficacy outcome
In rat hippocampal cultures, NAP increased synaptophysin expression.
In plain wordsIn cells from rat hippocampus, NAP increased synaptophysin expression.
Peptide
Davunetide
Outcome
IncreaseHippocampal synaptophysin expression
Study
ratn: not reported in source
Rat hippocampal cultures
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
Finally, we found that ADNF-9 and NAP increased synaptophysin expression in both rat hippocampal and cortical cultures.
1 context sentence(design, population, supporting numbers)
Summary · context
The purpose of the present studies was to determine if ADNF-9 or NAP affects neurite outgrowth and synaptogenesis in rat hippocampal and cortical cultures.
Report a problemFND-1580037605
06LabIn vitro (cells)Efficacy outcome
In rat cortical cultures, NAP increased synaptophysin expression.
In plain wordsIn cells from rat cortex, NAP increased synaptophysin expression.
Peptide
Davunetide
Outcome
IncreaseCortical synaptophysin expression
Study
ratn: not reported in source
Rat cortical cultures
Outcome group
Neuroprotection · Nervous system and cognition
Studied dose
Dose not stated
Source sentences
Summary
Finally, we found that ADNF-9 and NAP increased synaptophysin expression in both rat hippocampal and cortical cultures.
1 context sentence(design, population, supporting numbers)
Summary · context
The purpose of the present studies was to determine if ADNF-9 or NAP affects neurite outgrowth and synaptogenesis in rat hippocampal and cortical cultures.
Report a problemFND-1580037606

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Registered trials2

Trials this study reports on, or that list this study as a reference. Registry data from ClinicalTrials.gov.

2 trials · 2 completed · 1 with posted results · source ClinicalTrials.gov (opens in a new tab)

CompletedPhase 1NCT01056965

Davunetide (AL-108) in Predicted Tauopathies - Pilot Study

University of California, San Franciscon = 12Jan 2010 to Dec 2012

Predicted Tauopathies, Including · Progressive Supranuclear Palsy · Frontotemporal Dementia With Parkinsonism Linked to Chromosome 17 · Corticobasal Degeneration Syndrome · Progressive Nonfluent Aphasia

Trial page ClinicalTrials.govUpdated 5 Apr 2019

CompletedPhase 2Results postedNCT00505765

A Multicenter Study of NAP (AL-108) in Schizophrenia

University of California, Los Angelesn = 63Jul 2007 to Apr 2009

Schizophrenia

Trial page ClinicalTrials.govUpdated 10 Mar 2017

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
27 Oct 2026, 18:20 UTC
Study results updated
Version 1
27 Oct 2026, 16:32 UTC
First added · source update from Europe PMC

Subjects

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