Effects of dietary protein and growth hormone-releasing peptide (GHRP-2) on plasma IGF-1 and IGFBPs in Holstein steers.

Lee HG, Choi YJ, Lee SR, Kuwayama H, Hidari H, You SK

Domestic animal endocrinology1 Feb 2005Cited by 9 (Europe PMC)

PMID15713362DOI10.1016/j.domaniend.2004.07.001PepBankPUB-15713362

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Summary

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Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

6 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Efficacy outcome
In Holstein steers, the maximum GH peaks after GHRP‑2 injection were higher at day 1 than at day 6, as they were for saline.
In plain wordsIn steers, GH peaks after GHRP‑2 were higher on day 1 than on day 6. The same happened with saline.
Peptide
GHRP-2
Outcome
DecreaseGH peak amplitude after GHRP-2 injection, day 1 versus day 6
Study
Cattle (Holstein steers)n = 8
Holstein steers on high or low protein diet
Comparator
Day 6
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
12.5 microg/kg BW/day, injected twice daily · intravenous (jugular vein) · 6 daysAs given in this study (Cattle (Holstein steers)).
Source sentences
Summary
The amplitude of the maximum growth hormone (GH) peaks responding to GHRP-2 injection were higher at day 1 than at day 6 of saline or GHRP-2 treatment in both LP and HP steers (P<0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
Eight 16-month-old Holstein steers were grouped by liveweight to two feeding treatments; high protein (HP; CP 1.38 kg/day and TDN 4.5 kg/day DM intake, n=4) or low protein (LP; CP 0.66 kg/day and TDN 4.42 kg/day DM intake, n=4).
Summary · context
The experiment was a single reverse design whereby each group was injected twice daily with GHRP-2 (12.5 microg/kg body weight (BW)/day) or saline solution into the jugular vein for a 6-day period.
Report a problemFND-1571336203
02AnimalAnimal study (in vivo)Efficacy outcome
In Holstein steers, the GH response area after GHRP‑2 injection did not differ between low and high protein diets at days 1 and 6.
In plain wordsIn steers, the GH response to GHRP‑2 was not different between low-protein and high-protein diets.
Peptide
GHRP-2
Outcome
No significant changeGH response area under the curve, low versus high protein diet
Study
Cattle (Holstein steers)n = 8
Holstein steers on high or low protein diet
Comparator
Low-protein diet
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
12.5 microg/kg BW/day, injected twice daily · intravenous (jugular vein) · 6 daysAs given in this study (Cattle (Holstein steers)).
Source sentences
Summary
The area under the GH response curve for 180 min after the GHRP-2 injection was not significantly different between the LP and the HP groups at days 1 and 6.
2 context sentences(design, population, supporting numbers)
Summary · context
Eight 16-month-old Holstein steers were grouped by liveweight to two feeding treatments; high protein (HP; CP 1.38 kg/day and TDN 4.5 kg/day DM intake, n=4) or low protein (LP; CP 0.66 kg/day and TDN 4.42 kg/day DM intake, n=4).
Summary · context
The experiment was a single reverse design whereby each group was injected twice daily with GHRP-2 (12.5 microg/kg body weight (BW)/day) or saline solution into the jugular vein for a 6-day period.
Report a problemFND-1571336204
03AnimalAnimal study (in vivo)Efficacy outcome
In Holstein steers, GHRP‑2 had no apparent effect on plasma 38-43 kDa IGFBP-3 at days 2 and 6.
In plain wordsIn steers, GHRP‑2 had no clear effect on blood IGFBP-3 levels.
Peptide
GHRP-2
Outcome
No significant changePlasma IGFBP-3 response
Study
Cattle (Holstein steers)n = 8
Holstein steers on high or low protein diet
Comparator
Saline
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
12.5 microg/kg BW/day, injected twice daily · intravenous (jugular vein) · 6 daysAs given in this study (Cattle (Holstein steers)).
Source sentences
Summary
there was no apparent effect of GHRP-2 treatment on plasma 38-43 kDa IGFBP-3 and 34 kDa IGFBP-2 at days 2 and 6 of treatment.
2 context sentences(design, population, supporting numbers)
Summary · context
Eight 16-month-old Holstein steers were grouped by liveweight to two feeding treatments; high protein (HP; CP 1.38 kg/day and TDN 4.5 kg/day DM intake, n=4) or low protein (LP; CP 0.66 kg/day and TDN 4.42 kg/day DM intake, n=4).
Summary · context
The experiment was a single reverse design whereby each group was injected twice daily with GHRP-2 (12.5 microg/kg body weight (BW)/day) or saline solution into the jugular vein for a 6-day period.
Report a problemFND-1571336205
04AnimalAnimal study (in vivo)Efficacy outcome
In Holstein steers, GHRP‑2 had no apparent effect on plasma 34 kDa IGFBP-2 at days 2 and 6.
In plain wordsIn steers, GHRP‑2 had no clear effect on blood IGFBP-2 levels.
Peptide
GHRP-2
Outcome
No significant changePlasma IGFBP-2 response
Study
Cattle (Holstein steers)n = 8
Holstein steers on high or low protein diet
Comparator
Saline
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
12.5 microg/kg BW/day, injected twice daily · intravenous (jugular vein) · 6 daysAs given in this study (Cattle (Holstein steers)).
Source sentences
Summary
there was no apparent effect of GHRP-2 treatment on plasma 38-43 kDa IGFBP-3 and 34 kDa IGFBP-2 at days 2 and 6 of treatment.
2 context sentences(design, population, supporting numbers)
Summary · context
Eight 16-month-old Holstein steers were grouped by liveweight to two feeding treatments; high protein (HP; CP 1.38 kg/day and TDN 4.5 kg/day DM intake, n=4) or low protein (LP; CP 0.66 kg/day and TDN 4.42 kg/day DM intake, n=4).
Summary · context
The experiment was a single reverse design whereby each group was injected twice daily with GHRP-2 (12.5 microg/kg body weight (BW)/day) or saline solution into the jugular vein for a 6-day period.
Report a problemFND-1571336206
05AnimalAnimal study (in vivo)Efficacy outcome
In Holstein steers on a high-protein diet, GHRP‑2 increased plasma IGF-1 at days 1, 2 and 6.
In plain wordsIn steers fed a high-protein diet, GHRP‑2 raised blood IGF-1 levels.
Peptide
GHRP-2
Outcome
IncreasePlasma IGF-1 response in high-protein group
Study
Cattle (Holstein steers)n = 4
Holstein steers on high or low protein diet
Comparator
Saline
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
12.5 microg/kg BW/day, injected twice daily · intravenous (jugular vein) · 6 daysAs given in this study (Cattle (Holstein steers)).
Measurements
Plasma IGF-1
198.9 ng/ml
high protein, day 1
Plasma IGF-1
195.2 ng/ml
high protein, day 2
Plasma IGF-1
201.3 ng/ml
high protein, day 6
Source sentences
Summary
A response in plasma IGF-1 concentration to GHRP-2 treatment in the HP group was observed at day 1 (198.9+/-18.1 ng/ml), day 2 (195.2+/-21.1 ng/ml) and day 6 (201.3+/-14.8 ng/ml) (P<0.05).
2 context sentences(design, population, supporting numbers)
Summary · context
Eight 16-month-old Holstein steers were grouped by liveweight to two feeding treatments; high protein (HP; CP 1.38 kg/day and TDN 4.5 kg/day DM intake, n=4) or low protein (LP; CP 0.66 kg/day and TDN 4.42 kg/day DM intake, n=4).
Summary · context
The experiment was a single reverse design whereby each group was injected twice daily with GHRP-2 (12.5 microg/kg body weight (BW)/day) or saline solution into the jugular vein for a 6-day period.
Report a problemFND-1571336201
06AnimalAnimal study (in vivo)Efficacy outcome
In Holstein steers on a low-protein diet, GHRP‑2 did not increase plasma IGF-1.
In plain wordsIn steers fed a low-protein diet, GHRP‑2 did not raise blood IGF-1 levels.
Peptide
GHRP-2
Outcome
No significant changePlasma IGF-1 response in low-protein group
Study
Cattle (Holstein steers)n = 4
Holstein steers on high or low protein diet
Comparator
Saline
Outcome group
Growth hormone and IGF-1 · Endocrine and hormones
Studied dose
12.5 microg/kg BW/day, injected twice daily · intravenous (jugular vein) · 6 daysAs given in this study (Cattle (Holstein steers)).
Source sentences
Summary
No increase in plasma IGF-1 was observed from GHRP-2 administration in the LP group.
2 context sentences(design, population, supporting numbers)
Summary · context
Eight 16-month-old Holstein steers were grouped by liveweight to two feeding treatments; high protein (HP; CP 1.38 kg/day and TDN 4.5 kg/day DM intake, n=4) or low protein (LP; CP 0.66 kg/day and TDN 4.42 kg/day DM intake, n=4).
Summary · context
The experiment was a single reverse design whereby each group was injected twice daily with GHRP-2 (12.5 microg/kg body weight (BW)/day) or saline solution into the jugular vein for a 6-day period.
Report a problemFND-1571336202

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Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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