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Study · In vitro (cells) · 2004

2 results

Effects of pH, salt and time on ligand binding properties of overexpressed melanocortin 4 receptor.

Dolby V, Lundqvist A, Fröberg T, Lüllau E, Shaw J, Tjerneld F, Cronet P

Journal of biochemical and biophysical methods1 Mar 2004Cited by 1 (Europe PMC)

PMID15026206 (opens in a new tab)DOI10.1016/j.jbbm.2003.10.007 (opens in a new tab)PepBankPUB-15026206
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In membranes of CHO cells overexpressing MC4r, MTII affinity was lost below pH 6 and was restored by returning to physiological pH.

    In plain wordsIn cell membranes, melanotan II stopped binding its receptor below pH 6, and binding returned when the pH went back to normal.

    Peptide
    Melanotan II
    Outcome
    DecreaseMTII binding affinity at different pH
    Study

    n: not reported in source

    Membranes of CHO cells overexpressing MC4r
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    The affinity for the ligands [Nle4, d-Phe7]-alphaMSH and MTII was lost below pH 6 but could be restored by returning to physiological pH.
    1 context sentence
    Summary · context
    We overexpressed the MC4r in CHO cells and performed characterisation studies on the cell membranes to determine functional stability and ligand binding properties of the receptor.
  2. 02LabIn vitro (cells)Activity measurement

    In membranes of CHO cells overexpressing MC4r, NaCl up to 1 M had little influence on the binding of MTII.

    In plain wordsIn cell membranes, salt up to 1 M had little effect on how melanotan II bound its receptor.

    Peptide
    Melanotan II
    Outcome
    No significant changeMTII binding at increasing NaCl concentration
    Study

    n: not reported in source

    Membranes of CHO cells overexpressing MC4r
    Outcome group
    Receptor activity and mechanism · Pharmacology
    Studied dose
    Dose not stated

    Source sentences

    Summary
    Increasing NaCl concentration up to 1 M had little influence on the binding of either ligand.
    1 context sentence
    Summary · context
    We overexpressed the MC4r in CHO cells and performed characterisation studies on the cell membranes to determine functional stability and ligand binding properties of the receptor.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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