Interaction of the growth hormone-releasing peptides ghrelin and growth hormone-releasing peptide-6 with the motilin receptor in the rabbit gastric antrum.

Depoortere I, Thijs T, Thielemans L, Robberecht P, Peeters TL

The Journal of pharmacology and experimental therapeutics11 Feb 2003Cited by 55 (Europe PMC)

PMID12606621DOI10.1124/jpet.102.047563PepBankPUB-12606621

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study8

7 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueEfficacy outcome
In rabbit antral muscle strips, GHRP‑6 did not induce smooth muscle contractile responses.
In plain wordsIn rabbit stomach muscle strips, GHRP‑6 did not cause the muscle to contract on its own.
Peptide
GHRP-6
Outcome
No significant changeSmooth muscle contractile responses
Study
rabbitn: not reported in source
antral muscle strips
Outcome group
Gastrointestinal motility · Gastrointestinal
Studied dose
Dose not stated · bath applicationAs given in this study (rabbit).
Source sentences
Summary
Motilin induced smooth muscle contractile responses but ghrelin and GHRP-6 were ineffective.
1 context sentence(design, population, supporting numbers)
Summary · context
The interaction was evaluated in the rabbit gastric antrum with binding studies on membrane preparations and with contraction studies on muscle strips in the presence of selective antagonists under conditions of electrical field stimulation (EFS) or not.
Report a problemFND-1260662102
02LabEx vivo tissueEfficacy outcome
In rabbit antral muscle strips, GHRP‑6 enhanced the on-responses to electrical field stimulation.
In plain wordsIn rabbit stomach muscle strips, GHRP‑6 strengthened the first response to nerve stimulation.
Peptide
GHRP-6
Outcome
IncreaseOn-responses to electrical field stimulation
Study
rabbitn: not reported in source
antral muscle strips
Outcome group
Gastrointestinal motility · Gastrointestinal
Studied dose
Dose not stated · bath applicationAs given in this study (rabbit).
Source sentences
Summary
In contrast, GHRP-6 also enhanced the on- and off-responses.
1 context sentence(design, population, supporting numbers)
Summary · context
The interaction was evaluated in the rabbit gastric antrum with binding studies on membrane preparations and with contraction studies on muscle strips in the presence of selective antagonists under conditions of electrical field stimulation (EFS) or not.
Report a problemFND-1260662103
03LabEx vivo tissueEfficacy outcome
In rabbit antral muscle strips, GHRP‑6 enhanced the off-responses to electrical field stimulation.
In plain wordsIn rabbit stomach muscle strips, GHRP‑6 strengthened the response that follows nerve stimulation.
Peptide
GHRP-6
Outcome
IncreaseOff-responses to electrical field stimulation
Study
rabbitn: not reported in source
antral muscle strips
Outcome group
Gastrointestinal motility · Gastrointestinal
Studied dose
Dose not stated · bath applicationAs given in this study (rabbit).
Source sentences
Summary
In contrast, GHRP-6 also enhanced the on- and off-responses.
1 context sentence(design, population, supporting numbers)
Summary · context
The interaction was evaluated in the rabbit gastric antrum with binding studies on membrane preparations and with contraction studies on muscle strips in the presence of selective antagonists under conditions of electrical field stimulation (EFS) or not.
Report a problemFND-1260662104
04LabEx vivo tissueMechanism
In rabbit antral strips, the motilin antagonist GM-109 blocked the effect of GHRP‑6 on off-responses but not on on-responses.
In plain wordsIn rabbit stomach muscle strips, a motilin blocker stopped GHRP‑6 from acting on the off-response but not on the on-response.
Peptide
GHRP-6
Outcome
Not applicableEffect of motilin antagonist GM-109 on GHRP-6 responses
Study
rabbitn: not reported in source
antral muscle strips
Outcome group
Gastrointestinal motility · Gastrointestinal
Studied dose
Dose not stated · bath applicationAs given in this study (rabbit).
Source sentences
Summary
The motilin antagonist Phe-cyclo[Lys-Tyr(3-tBu)-betaAla-] trifluoroacetate (GM-109) blocked the effect of GHRP-6 on the off-responses but not on the on-responses.
1 context sentence(design, population, supporting numbers)
Summary · context
The interaction was evaluated in the rabbit gastric antrum with binding studies on membrane preparations and with contraction studies on muscle strips in the presence of selective antagonists under conditions of electrical field stimulation (EFS) or not.
Report a problemFND-1260662105
05LabEx vivo tissueMechanism
In rabbit antral strips under nonadrenergic noncholinergic conditions, the effect of GHRP‑6 on on-responses was abolished.
In plain wordsIn rabbit stomach muscle strips, GHRP‑6 no longer affected the on-response once certain nerve pathways were blocked.
Peptide
GHRP-6
Outcome
DecreaseGHRP-6 on-responses under nonadrenergic noncholinergic conditions
Study
rabbitn: not reported in source
antral muscle strips
Outcome group
Gastrointestinal motility · Gastrointestinal
Studied dose
Dose not stated · bath applicationAs given in this study (rabbit).
Source sentences
Summary
Under nonadrenergic noncholinergic conditions, the effects of motilin and GHRP-6 on the on-responses were abolished; those on the off-responses were preserved.
1 context sentence(design, population, supporting numbers)
Summary · context
The interaction was evaluated in the rabbit gastric antrum with binding studies on membrane preparations and with contraction studies on muscle strips in the presence of selective antagonists under conditions of electrical field stimulation (EFS) or not.
Report a problemFND-1260662106
06LabEx vivo tissueMechanism
In rabbit antral strips under nonadrenergic noncholinergic conditions, the effect of GHRP‑6 on off-responses was preserved.
In plain wordsIn rabbit stomach muscle strips, GHRP‑6 still affected the off-response once certain nerve pathways were blocked.
Peptide
GHRP-6
Outcome
No significant changeGHRP-6 off-responses under nonadrenergic noncholinergic conditions
Study
rabbitn: not reported in source
antral muscle strips
Outcome group
Gastrointestinal motility · Gastrointestinal
Studied dose
Dose not stated · bath applicationAs given in this study (rabbit).
Source sentences
Summary
Under nonadrenergic noncholinergic conditions, the effects of motilin and GHRP-6 on the on-responses were abolished; those on the off-responses were preserved.
1 context sentence(design, population, supporting numbers)
Summary · context
The interaction was evaluated in the rabbit gastric antrum with binding studies on membrane preparations and with contraction studies on muscle strips in the presence of selective antagonists under conditions of electrical field stimulation (EFS) or not.
Report a problemFND-1260662107
07LabIn vitro (cell-free)Activity measurement
In rabbit antral membranes, GHRP‑6 bound the motilin receptor with a pKd of 5.54, lower than motilin (9.13) and higher than ghrelin (4.23).
In plain wordsIn lab tests, GHRP‑6 bound the motilin receptor more weakly than motilin itself and more strongly than ghrelin.
Peptide
GHRP-6
Outcome
Not applicableMotilin receptor affinity (pKd)
Study
rabbitn: not reported in source
rabbit antral membrane preparations
Outcome group
Receptor activity and mechanism · Pharmacology
Studied dose
Dose not stated
Measurements
pKd
5.54 pKd
GHRP-6, motilin receptor
Source sentences
Summary
Binding studies indicated that the affinity (pK(d)) for the motilin receptor was in the order of ghrelin (4.23 +/- 0.07) < GHRP-6 (5.54 +/- 0.08) < motilin (9.13 +/- 0.03).
1 context sentence(design, population, supporting numbers)
Summary · context
The interaction was evaluated in the rabbit gastric antrum with binding studies on membrane preparations and with contraction studies on muscle strips in the presence of selective antagonists under conditions of electrical field stimulation (EFS) or not.
Report a problemFND-1260662101

Studies that did not test the peptide alone1

In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-6.

08LabNot a test of GHRP-6 aloneEx vivo tissueMechanism
In rabbit antral strips, all responses (motilin and GHRP‑6, pooled) were blocked by neurokinin NK1 and NK2 antagonists.
In plain wordsIn rabbit stomach muscle strips, all the responses to motilin and GHRP‑6, pooled, were blocked by tachykinin blockers.
Peptide
GHRP-6
Outcome
DecreaseResponses blocked by NK1 and NK2 antagonists
Study
rabbitn: not reported in source
antral muscle strips
Outcome group
Gastrointestinal motility · Gastrointestinal
Source sentences
Summary
All responses were blocked by neurokinin (NK)(1) and NK(2) antagonists.
2 context sentences(design, population, supporting numbers)
Summary · context
Under nonadrenergic noncholinergic conditions, the effects of motilin and GHRP-6 on the on-responses were abolished; those on the off-responses were preserved.
Summary · context
The interaction was evaluated in the rabbit gastric antrum with binding studies on membrane preparations and with contraction studies on muscle strips in the presence of selective antagonists under conditions of electrical field stimulation (EFS) or not.
In this study, GHRP-6 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-6.
Report a problemFND-1260662108

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
12 Oct 2026, 12:58 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

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Interaction of the growth hormone-releasing peptides ghrelin and growth hormone-releasing peptide-6 with the motilin receptor in the rabbit gastric antrum.

Summary

Results from this study8

Studies that did not test the peptide alone1

Version history

Subjects

Publication types

MeSH terms