Oocyte-mediated suppression of follicle-stimulating hormone- and insulin-like growth factor-induced secretion of steroids and inhibin-related proteins by bovine granulosa cells in vitro: possible role of transforming growth factor alpha.

Glister C, Groome NP, Knight PG

Biology of reproduction1 Mar 2003Cited by 39 (Europe PMC)

PMID12604623DOI10.1095/biolreprod.102.008698PepBankPUB-12604623

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study11

6 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Activity measurement
In cultured bovine granulosa cells, the IGF analogue LR3-IGF-1 increased inhibin A secretion.
In plain wordsIn cells from cows, the IGF analogue raised the secretion of inhibin A.
Peptide
IGF-1 LR3
Outcome
IncreaseInhibin A secretion
Study
bovinen: not reported in source
Bovine granulosa cells in vitro
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Both FSH and IGF increased (P < 0.001) secretion of inh A, act A, FS, E(2), and P(4) and raised cell number.
1 context sentence(design, population, supporting numbers)
Summary · context
Cells from 4- to 6-mm follicles were cultured in serum-free medium containing insulin and androstenedione, and the effects of ovine FSH and IGF analogue (LR3-IGF-1) were tested alone and in the presence of denuded bovine oocytes (2, 8, or 20 per well).
Report a problemFND-1260462301
02LabIn vitro (cells)Activity measurement
In cultured bovine granulosa cells, the IGF analogue LR3-IGF-1 increased activin A secretion.
In plain wordsIn cells from cows, the IGF analogue raised the secretion of activin A.
Peptide
IGF-1 LR3
Outcome
IncreaseActivin A secretion
Study
bovinen: not reported in source
Bovine granulosa cells in vitro
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Both FSH and IGF increased (P < 0.001) secretion of inh A, act A, FS, E(2), and P(4) and raised cell number.
1 context sentence(design, population, supporting numbers)
Summary · context
Cells from 4- to 6-mm follicles were cultured in serum-free medium containing insulin and androstenedione, and the effects of ovine FSH and IGF analogue (LR3-IGF-1) were tested alone and in the presence of denuded bovine oocytes (2, 8, or 20 per well).
Report a problemFND-1260462302
03LabIn vitro (cells)Activity measurement
In cultured bovine granulosa cells, the IGF analogue LR3-IGF-1 increased follistatin secretion.
In plain wordsIn cells from cows, the IGF analogue raised the secretion of follistatin.
Peptide
IGF-1 LR3
Outcome
IncreaseFollistatin secretion
Study
bovinen: not reported in source
Bovine granulosa cells in vitro
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Both FSH and IGF increased (P < 0.001) secretion of inh A, act A, FS, E(2), and P(4) and raised cell number.
1 context sentence(design, population, supporting numbers)
Summary · context
Cells from 4- to 6-mm follicles were cultured in serum-free medium containing insulin and androstenedione, and the effects of ovine FSH and IGF analogue (LR3-IGF-1) were tested alone and in the presence of denuded bovine oocytes (2, 8, or 20 per well).
Report a problemFND-1260462303
04LabIn vitro (cells)Activity measurement
In cultured bovine granulosa cells, the IGF analogue LR3-IGF-1 increased estradiol secretion.
In plain wordsIn cells from cows, the IGF analogue raised the secretion of estradiol.
Peptide
IGF-1 LR3
Outcome
IncreaseEstradiol secretion
Study
bovinen: not reported in source
Bovine granulosa cells in vitro
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Both FSH and IGF increased (P < 0.001) secretion of inh A, act A, FS, E(2), and P(4) and raised cell number.
1 context sentence(design, population, supporting numbers)
Summary · context
Cells from 4- to 6-mm follicles were cultured in serum-free medium containing insulin and androstenedione, and the effects of ovine FSH and IGF analogue (LR3-IGF-1) were tested alone and in the presence of denuded bovine oocytes (2, 8, or 20 per well).
Report a problemFND-1260462304
05LabIn vitro (cells)Activity measurement
In cultured bovine granulosa cells, the IGF analogue LR3-IGF-1 increased progesterone secretion.
In plain wordsIn cells from cows, the IGF analogue raised the secretion of progesterone.
Peptide
IGF-1 LR3
Outcome
IncreaseProgesterone secretion
Study
bovinen: not reported in source
Bovine granulosa cells in vitro
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Both FSH and IGF increased (P < 0.001) secretion of inh A, act A, FS, E(2), and P(4) and raised cell number.
1 context sentence(design, population, supporting numbers)
Summary · context
Cells from 4- to 6-mm follicles were cultured in serum-free medium containing insulin and androstenedione, and the effects of ovine FSH and IGF analogue (LR3-IGF-1) were tested alone and in the presence of denuded bovine oocytes (2, 8, or 20 per well).
Report a problemFND-1260462305
06LabIn vitro (cells)Activity measurement
In cultured bovine granulosa cells, the IGF analogue LR3-IGF-1 raised cell number.
In plain wordsIn cells from cows, the IGF analogue increased the number of cells.
Peptide
IGF-1 LR3
Outcome
IncreaseGranulosa cell number
Study
bovinen: not reported in source
Bovine granulosa cells in vitro
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Studied dose
Dose not stated
Source sentences
Summary
Both FSH and IGF increased (P < 0.001) secretion of inh A, act A, FS, E(2), and P(4) and raised cell number.
1 context sentence(design, population, supporting numbers)
Summary · context
Cells from 4- to 6-mm follicles were cultured in serum-free medium containing insulin and androstenedione, and the effects of ovine FSH and IGF analogue (LR3-IGF-1) were tested alone and in the presence of denuded bovine oocytes (2, 8, or 20 per well).
Report a problemFND-1260462306

Studies that did not test the peptide alone5

In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking IGF-1 LR3.

07LabNot a test of IGF-1 LR3 aloneIn vitro (cells)Activity measurement
In cultured bovine granulosa cells, coculture with oocytes suppressed the inhibin A secretion that LR3-IGF-1 induced.
In plain wordsIn cells from cows, oocytes lowered the inhibin A rise that the IGF analogue caused.
Peptide
IGF-1 LR3
Outcome
DecreaseIGF-induced inhibin A secretion with oocytes
Study
bovinen: not reported in source
Bovine granulosa cells in vitro
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Likewise, oocytes suppressed (P < 0.001) IGF-induced secretion of inh A, act A, FS, and E(2) (P < 0.05) but enhanced IGF-induced P(4) secretion (1.7-fold; P < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Cells from 4- to 6-mm follicles were cultured in serum-free medium containing insulin and androstenedione, and the effects of ovine FSH and IGF analogue (LR3-IGF-1) were tested alone and in the presence of denuded bovine oocytes (2, 8, or 20 per well).
In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.
Report a problemFND-1260462307
08LabNot a test of IGF-1 LR3 aloneIn vitro (cells)Activity measurement
In cultured bovine granulosa cells, coculture with oocytes suppressed the activin A secretion that LR3-IGF-1 induced.
In plain wordsIn cells from cows, oocytes lowered the activin A rise that the IGF analogue caused.
Peptide
IGF-1 LR3
Outcome
DecreaseIGF-induced activin A secretion with oocytes
Study
bovinen: not reported in source
Bovine granulosa cells in vitro
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Likewise, oocytes suppressed (P < 0.001) IGF-induced secretion of inh A, act A, FS, and E(2) (P < 0.05) but enhanced IGF-induced P(4) secretion (1.7-fold; P < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Cells from 4- to 6-mm follicles were cultured in serum-free medium containing insulin and androstenedione, and the effects of ovine FSH and IGF analogue (LR3-IGF-1) were tested alone and in the presence of denuded bovine oocytes (2, 8, or 20 per well).
In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.
Report a problemFND-1260462308
09LabNot a test of IGF-1 LR3 aloneIn vitro (cells)Activity measurement
In cultured bovine granulosa cells, coculture with oocytes suppressed the follistatin secretion that LR3-IGF-1 induced.
In plain wordsIn cells from cows, oocytes lowered the follistatin rise that the IGF analogue caused.
Peptide
IGF-1 LR3
Outcome
DecreaseIGF-induced follistatin secretion with oocytes
Study
bovinen: not reported in source
Bovine granulosa cells in vitro
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Likewise, oocytes suppressed (P < 0.001) IGF-induced secretion of inh A, act A, FS, and E(2) (P < 0.05) but enhanced IGF-induced P(4) secretion (1.7-fold; P < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Cells from 4- to 6-mm follicles were cultured in serum-free medium containing insulin and androstenedione, and the effects of ovine FSH and IGF analogue (LR3-IGF-1) were tested alone and in the presence of denuded bovine oocytes (2, 8, or 20 per well).
In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.
Report a problemFND-1260462309
10LabNot a test of IGF-1 LR3 aloneIn vitro (cells)Activity measurement
In cultured bovine granulosa cells, coculture with oocytes suppressed the estradiol secretion that LR3-IGF-1 induced.
In plain wordsIn cells from cows, oocytes lowered the estradiol rise that the IGF analogue caused.
Peptide
IGF-1 LR3
Outcome
DecreaseIGF-induced estradiol secretion with oocytes
Study
bovinen: not reported in source
Bovine granulosa cells in vitro
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Source sentences
Summary
Likewise, oocytes suppressed (P < 0.001) IGF-induced secretion of inh A, act A, FS, and E(2) (P < 0.05) but enhanced IGF-induced P(4) secretion (1.7-fold; P < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Cells from 4- to 6-mm follicles were cultured in serum-free medium containing insulin and androstenedione, and the effects of ovine FSH and IGF analogue (LR3-IGF-1) were tested alone and in the presence of denuded bovine oocytes (2, 8, or 20 per well).
In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.
Report a problemFND-1260462310
11LabNot a test of IGF-1 LR3 aloneIn vitro (cells)Activity measurement
In cultured bovine granulosa cells, coculture with oocytes enhanced the progesterone secretion that LR3-IGF-1 induced.
In plain wordsIn cells from cows, oocytes raised the progesterone rise that the IGF analogue caused even more.
Peptide
IGF-1 LR3
Outcome
IncreaseIGF-induced progesterone secretion with oocytes
Study
bovinen: not reported in source
Bovine granulosa cells in vitro
Outcome group
Sex hormones and fertility · Reproductive and sexual health
Measurements
IGF-induced progesterone secretion enhancement
1.7-fold
with oocytes
Source sentences
Summary
Likewise, oocytes suppressed (P < 0.001) IGF-induced secretion of inh A, act A, FS, and E(2) (P < 0.05) but enhanced IGF-induced P(4) secretion (1.7-fold; P < 0.05).
1 context sentence(design, population, supporting numbers)
Summary · context
Cells from 4- to 6-mm follicles were cultured in serum-free medium containing insulin and androstenedione, and the effects of ovine FSH and IGF analogue (LR3-IGF-1) were tested alone and in the presence of denuded bovine oocytes (2, 8, or 20 per well).
In this study, IGF-1 LR3 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking IGF-1 LR3.
Report a problemFND-1260462311

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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