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Study · Not a test of the peptide alone · 2002

3 results

[Fusion expression of human thymosin alpha 1 in Escherichia coli].

Xiu ZY, Yu Y, Chen CQ

Sheng wu gong cheng xue bao = Chinese journal of biotechnology1 Sept 2002Cited by 2 (Europe PMC)

PMID12561195 (opens in a new tab)PepBankPUB-12561195
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone3

In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Thymosin alpha-1.

  1. 01LabNot a test of Thymosin alpha-1 aloneOther designOther

    In E. coli, the GST-thymosin alpha‑1 fusion protein reached about 35%-40% of total protein after fermentation.

    In plain wordsIn bacteria engineered to make it, a thymosin alpha‑1 fusion protein made up about 35%-40% of total protein.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableFusion protein expression level
    Study

    Escherichia colin: not reported in source

    E. coli BL21 (DE3) expression system
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    Fusion protein expression
    35 to 40 % of total protein
    GST-thymosin alpha 1, after fermentation

    Source sentences

    Summary
    The yield of fusion protein of GST-Thymosin alpha 1 expressed from BL21 (DE3)/pGEX-4T-thymosin alpha 1 is about 35%-40% of total protein after fermentation.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

  2. 02LabNot a test of Thymosin alpha-1 aloneOther designOther

    After cleavage with thrombin or CNBr, about 0.2 g/L thymosin alpha‑1 was harvested.

    In plain wordsAfter cutting the fusion protein, about 0.2 g/L of thymosin alpha‑1 was obtained.

    Peptide
    Thymosin alpha-1
    Outcome
    Not applicableYield of thymosin alpha 1 after cleavage
    Study

    Escherichia colin: not reported in source

    E. coli BL21 (DE3) expression system
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Measurements

    Yield
    ≈ 0.2 g/L
    after thrombin or CNBr cleavage

    Source sentences

    Summary
    Following the simple cut of thrombin or CNBr, about 0.2 g/L thymosin alpha 1 can be harvested.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

  3. 03LabNot a test of Thymosin alpha-1 aloneOther designActivity measurement

    The recombinant thymosin alpha‑1 had full biological activity of the native peptide in an activity test.

    In plain wordsIn a lab check, the made thymosin alpha‑1 had the full activity of the natural form.

    Peptide
    Thymosin alpha-1
    Outcome
    No significant changeBiological activity of recombinant product
    Study

    Escherichia colin: not reported in source

    E. coli BL21 (DE3) expression system
    Comparator
    Native thymosin alpha 1
    Outcome group
    Synthesis and manufacturing · Pharmacology

    Source sentences

    Summary
    The product is checked by MS and activity test, which indicates that the recombinant product has full biological activity of native thymosin alpha 1.

    In this study, Thymosin alpha-1 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Thymosin alpha-1.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 3Current

    12 Oct 2026, 17:28 UTC

    Study results updated

  2. Version 2

    9 Oct 2026, 03:03 UTC

    Study data updated · source update from Europe PMC

  3. Version 1

    9 Oct 2026, 03:02 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

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