Growth hormone-releasing peptide-2 reduces inward rectifying K+ currents via a PKA-cAMP-mediated signalling pathway in ovine somatotropes.

Xu R, Zhao Y, Chen C

The Journal of physiology1 Dec 2002Cited by 15 (Europe PMC)

PMID12456822DOI10.1113/jphysiol.2002.030916PepBankPUB-12456822

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study6

1 result from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In cultured ovine somatotropes, GHRP‑2 reversibly and significantly reduced the Kir current in bath solutions with 5 or 25 mM K+.
In plain wordsIn cultured sheep pituitary cells, GHRP‑2 reduced a potassium current that keeps the cell resting.
Peptide
GHRP-2
Outcome
DecreaseInward-rectifying K+ (Kir) current
Study
sheepn: not reported in source
Primary cultured ovine somatotropes, perforated patch clamp
Comparator
Before GHRP-2
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
100 nMAs given in this study (sheep).
Source sentences
Summary
Application of GHRP-2 (100 nM) reversibly and significantly reduced the Kir current in bath solutions with 5 or 25 mM K(+) in ovine somatotropes.
2 context sentences(design, population, supporting numbers)
Summary · context
We characterised the Kir current in primary cultured ovine somatotropes and examined the effect of growth hormone-releasing peptide-2 (GHRP-2) on this current and its related intracellular signalling pathways.
Summary · context
The Kir current was, in most cases, isolated using nystatin-perforated patch-clamp techniques.
Report a problemFND-1245682201

Studies that did not test the peptide alone5

In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-2.

02LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In cultured ovine somatotropes, the PKA inhibitor H89 totally abolished the GHRP‑2-induced reduction in the Kir current.
In plain wordsIn cultured sheep pituitary cells, a protein kinase A blocker stopped GHRP‑2 from reducing the potassium current.
Peptide
GHRP-2
Outcome
DecreaseGHRP-2-induced Kir reduction with H89
Study
sheepn: not reported in source
Primary cultured ovine somatotropes, perforated patch clamp
Comparator
GHRP-2 without inhibitor
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
In addition, we found that the reduction in the Kir current mediated by GHRP-2 was totally abolished by the pretreatments with H89 (1 microM) or Rp-cAMP (100 microM) or by intracellular dialysis of a specific protein kinase A (PKA) inhibitory peptide PKI (10 microM).
2 context sentences(design, population, supporting numbers)
Summary · context
We characterised the Kir current in primary cultured ovine somatotropes and examined the effect of growth hormone-releasing peptide-2 (GHRP-2) on this current and its related intracellular signalling pathways.
Summary · context
The Kir current was, in most cases, isolated using nystatin-perforated patch-clamp techniques.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1245682202
03LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In cultured ovine somatotropes, Rp-cAMP totally abolished the GHRP‑2-induced reduction in the Kir current.
In plain wordsIn cultured sheep pituitary cells, a cAMP blocker stopped GHRP‑2 from reducing the potassium current.
Peptide
GHRP-2
Outcome
DecreaseGHRP-2-induced Kir reduction with Rp-cAMP
Study
sheepn: not reported in source
Primary cultured ovine somatotropes, perforated patch clamp
Comparator
GHRP-2 without inhibitor
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
In addition, we found that the reduction in the Kir current mediated by GHRP-2 was totally abolished by the pretreatments with H89 (1 microM) or Rp-cAMP (100 microM) or by intracellular dialysis of a specific protein kinase A (PKA) inhibitory peptide PKI (10 microM).
2 context sentences(design, population, supporting numbers)
Summary · context
We characterised the Kir current in primary cultured ovine somatotropes and examined the effect of growth hormone-releasing peptide-2 (GHRP-2) on this current and its related intracellular signalling pathways.
Summary · context
The Kir current was, in most cases, isolated using nystatin-perforated patch-clamp techniques.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1245682203
04LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In cultured ovine somatotropes, intracellular dialysis of the PKA inhibitory peptide PKI totally abolished the GHRP‑2-induced reduction in the Kir current.
In plain wordsIn cultured sheep pituitary cells, a protein kinase A inhibitor peptide stopped GHRP‑2 from reducing the potassium current.
Peptide
GHRP-2
Outcome
DecreaseGHRP-2-induced Kir reduction with PKI
Study
sheepn: not reported in source
Primary cultured ovine somatotropes, perforated patch clamp
Comparator
GHRP-2 without inhibitor
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
In addition, we found that the reduction in the Kir current mediated by GHRP-2 was totally abolished by the pretreatments with H89 (1 microM) or Rp-cAMP (100 microM) or by intracellular dialysis of a specific protein kinase A (PKA) inhibitory peptide PKI (10 microM).
2 context sentences(design, population, supporting numbers)
Summary · context
We characterised the Kir current in primary cultured ovine somatotropes and examined the effect of growth hormone-releasing peptide-2 (GHRP-2) on this current and its related intracellular signalling pathways.
Summary · context
The Kir current was, in most cases, isolated using nystatin-perforated patch-clamp techniques.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1245682204
05LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In cultured ovine somatotropes, the PKC blocker chelerythrine did not change the GHRP‑2-induced decrease in the Kir current.
In plain wordsIn cultured sheep pituitary cells, a protein kinase C blocker did not change the potassium current reduction caused by GHRP‑2.
Peptide
GHRP-2
Outcome
No significant changeGHRP-2-induced Kir reduction with chelerythrine
Study
sheepn: not reported in source
Primary cultured ovine somatotropes, perforated patch clamp
Comparator
GHRP-2 without inhibitor
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
The specific PKC blocker chelerythrine (1 microM) or inhibitory peptide PKC(19-36) (10 microM) did not show any effects on the GHRP-2-induced decrease in the Kir current.
2 context sentences(design, population, supporting numbers)
Summary · context
We characterised the Kir current in primary cultured ovine somatotropes and examined the effect of growth hormone-releasing peptide-2 (GHRP-2) on this current and its related intracellular signalling pathways.
Summary · context
The Kir current was, in most cases, isolated using nystatin-perforated patch-clamp techniques.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1245682205
06LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In cultured ovine somatotropes, the inhibitory peptide PKC(19-36) did not change the GHRP‑2-induced decrease in the Kir current.
In plain wordsIn cultured sheep pituitary cells, a protein kinase C inhibitor peptide did not change the potassium current reduction caused by GHRP‑2.
Peptide
GHRP-2
Outcome
No significant changeGHRP-2-induced Kir reduction with PKC(19-36)
Study
sheepn: not reported in source
Primary cultured ovine somatotropes, perforated patch clamp
Comparator
GHRP-2 without inhibitor
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
The specific PKC blocker chelerythrine (1 microM) or inhibitory peptide PKC(19-36) (10 microM) did not show any effects on the GHRP-2-induced decrease in the Kir current.
2 context sentences(design, population, supporting numbers)
Summary · context
We characterised the Kir current in primary cultured ovine somatotropes and examined the effect of growth hormone-releasing peptide-2 (GHRP-2) on this current and its related intracellular signalling pathways.
Summary · context
The Kir current was, in most cases, isolated using nystatin-perforated patch-clamp techniques.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1245682206

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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