Expression and homologous regulation of GH secretagogue receptor mRNA in rat adrenal gland.

Barreiro ML, Pinilla L, Aguilar E, Tena-Sempere M

European journal of endocrinology1 Nov 2002Cited by 21 (Europe PMC)

PMID12444901DOI10.1530/eje.0.1470677PepBankPUB-12444901

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study7

7 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueMechanism
In rat adrenal tissue, GHRP‑6 inhibited the increase in GHS-R mRNA during serum-free incubation.
In plain wordsIn lab tests, GHRP‑6 reduced the receptor gene expression increase during serum-free incubation.
Peptide
GHRP-6
Outcome
DecreaseAdrenal GHS-R mRNA
Study
ratn: not reported in source
incubated adrenal tissue
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
10(-9)-10(-5) mol/lAs given in this study (rat).
Source sentences
Summary
The adrenal expression of the GHS-R message appeared under the regulation of homologous signals in vitro, as short-term incubation of adrenal samples in serum-free medium induced a significant increase in GHS-R mRNA levels that was inhibited by exposure to different doses of GHRP-6 (10(-9)-10(-5) mo…
2 context sentences(design, population, supporting numbers)
Summary · context
The aims of this study were to evaluate the expression and homologous regulation of the GHS-receptor (GHS-R) gene in rat adrenal and to assess the effects of synthetic (GH releasing peptide-6 - GHRP-6) and natural (ghrelin) ligands of GHS-R upon basal and ACTH-stimulated corticosterone secretion in vitro.
Summary · context
Evaluation of corticosterone secretion by incubated adrenal tIssue was carried out by specific RIA.
Report a problemFND-1244490101
02LabEx vivo tissueMechanism
In rat pituitary, GHRP‑6 showed the opposite pattern of GHS-R gene regulation to adrenal tissue.
In plain wordsIn lab tests, GHRP‑6 showed opposite receptor gene regulation in pituitary and adrenal tissue.
Peptide
GHRP-6
Outcome
IncreasePituitary GHS-R gene regulation
Study
ratn: not reported in source
pituitary
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
Dose not stated
Source sentences
Summary
Notably, an opposite pattern of homologous regulation of GHS-R gene expression was observed at the pituitary.
3 context sentences(design, population, supporting numbers)
Summary · context
The adrenal expression of the GHS-R message appeared under the regulation of homologous signals in vitro, as short-term incubation of adrenal samples in serum-free medium induced a significant increase in GHS-R mRNA levels that was inhibited by exposure to different doses of GHRP-6 (10(-9)-10(-5) mo…
Summary · context
The aims of this study were to evaluate the expression and homologous regulation of the GHS-receptor (GHS-R) gene in rat adrenal and to assess the effects of synthetic (GH releasing peptide-6 - GHRP-6) and natural (ghrelin) ligands of GHS-R upon basal and ACTH-stimulated corticosterone secretion in vitro.
Summary · context
Evaluation of corticosterone secretion by incubated adrenal tIssue was carried out by specific RIA.
Report a problemFND-1244490102
03LabEx vivo tissueMechanism
In rat adrenal tissue, GHRP‑6 did not alter basal corticosterone secretion.
In plain wordsIn lab tests, GHRP‑6 did not change basal corticosterone release.
Peptide
GHRP-6
Outcome
No significant changeBasal corticosterone secretion
Study
ratn: not reported in source
incubated adrenal tissue
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
10(-9)-10(-5) mol/lAs given in this study (rat).
Source sentences
Summary
Finally, short-term stimulation with increasing concentrations of GHRP-6 (10(-9)-10(-5) mol/l) or ghrelin (10(-7) mol/l) failed to alter basal and ACTH-stimulated corticosterone secretion in vitro,
2 context sentences(design, population, supporting numbers)
Summary · context
The aims of this study were to evaluate the expression and homologous regulation of the GHS-receptor (GHS-R) gene in rat adrenal and to assess the effects of synthetic (GH releasing peptide-6 - GHRP-6) and natural (ghrelin) ligands of GHS-R upon basal and ACTH-stimulated corticosterone secretion in vitro.
Summary · context
Evaluation of corticosterone secretion by incubated adrenal tIssue was carried out by specific RIA.
Report a problemFND-1244490103
04LabEx vivo tissueMechanism
In rat adrenal tissue, GHRP‑6 did not alter ACTH-stimulated corticosterone secretion.
In plain wordsIn lab tests, GHRP‑6 did not change ACTH-stimulated corticosterone release.
Peptide
GHRP-6
Outcome
No significant changeACTH-stimulated corticosterone secretion
Study
ratn: not reported in source
incubated adrenal tissue
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
10(-9)-10(-5) mol/lAs given in this study (rat).
Source sentences
Summary
Finally, short-term stimulation with increasing concentrations of GHRP-6 (10(-9)-10(-5) mol/l) or ghrelin (10(-7) mol/l) failed to alter basal and ACTH-stimulated corticosterone secretion in vitro,
2 context sentences(design, population, supporting numbers)
Summary · context
The aims of this study were to evaluate the expression and homologous regulation of the GHS-receptor (GHS-R) gene in rat adrenal and to assess the effects of synthetic (GH releasing peptide-6 - GHRP-6) and natural (ghrelin) ligands of GHS-R upon basal and ACTH-stimulated corticosterone secretion in vitro.
Summary · context
Evaluation of corticosterone secretion by incubated adrenal tIssue was carried out by specific RIA.
Report a problemFND-1244490104
05LabEx vivo tissueMechanism
In rat adrenal tissue, GHRP‑6 did not modify ACTH-stimulated StAR mRNA expression.
In plain wordsIn lab tests, GHRP‑6 did not change ACTH-stimulated StAR gene expression.
Peptide
GHRP-6
Outcome
No significant changeACTH-stimulated StAR mRNA
Study
ratn: not reported in source
incubated adrenal tissue
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
10(-9)-10(-5) mol/lAs given in this study (rat).
Source sentences
Summary
neither did it modify ACTH-stimulated mRNA expression levels of several upstream elements in the steroidogenic route: the steroidogenic acute regulatory (StAR) protein, and the enzymes P450 cholesterol side-chain cleavage (P450scc) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD).
2 context sentences(design, population, supporting numbers)
Summary · context
The aims of this study were to evaluate the expression and homologous regulation of the GHS-receptor (GHS-R) gene in rat adrenal and to assess the effects of synthetic (GH releasing peptide-6 - GHRP-6) and natural (ghrelin) ligands of GHS-R upon basal and ACTH-stimulated corticosterone secretion in vitro.
Summary · context
Evaluation of corticosterone secretion by incubated adrenal tIssue was carried out by specific RIA.
Report a problemFND-1244490105
06LabEx vivo tissueMechanism
In rat adrenal tissue, GHRP‑6 did not modify ACTH-stimulated P450scc mRNA expression.
In plain wordsIn lab tests, GHRP‑6 did not change ACTH-stimulated P450scc gene expression.
Peptide
GHRP-6
Outcome
No significant changeACTH-stimulated P450scc mRNA
Study
ratn: not reported in source
incubated adrenal tissue
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
10(-9)-10(-5) mol/lAs given in this study (rat).
Source sentences
Summary
neither did it modify ACTH-stimulated mRNA expression levels of several upstream elements in the steroidogenic route: the steroidogenic acute regulatory (StAR) protein, and the enzymes P450 cholesterol side-chain cleavage (P450scc) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD).
2 context sentences(design, population, supporting numbers)
Summary · context
The aims of this study were to evaluate the expression and homologous regulation of the GHS-receptor (GHS-R) gene in rat adrenal and to assess the effects of synthetic (GH releasing peptide-6 - GHRP-6) and natural (ghrelin) ligands of GHS-R upon basal and ACTH-stimulated corticosterone secretion in vitro.
Summary · context
Evaluation of corticosterone secretion by incubated adrenal tIssue was carried out by specific RIA.
Report a problemFND-1244490106
07LabEx vivo tissueMechanism
In rat adrenal tissue, GHRP‑6 did not modify ACTH-stimulated 3beta-HSD mRNA expression.
In plain wordsIn lab tests, GHRP‑6 did not change ACTH-stimulated 3beta-HSD gene expression.
Peptide
GHRP-6
Outcome
No significant changeACTH-stimulated 3beta-HSD mRNA
Study
ratn: not reported in source
incubated adrenal tissue
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
10(-9)-10(-5) mol/lAs given in this study (rat).
Source sentences
Summary
neither did it modify ACTH-stimulated mRNA expression levels of several upstream elements in the steroidogenic route: the steroidogenic acute regulatory (StAR) protein, and the enzymes P450 cholesterol side-chain cleavage (P450scc) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD).
2 context sentences(design, population, supporting numbers)
Summary · context
The aims of this study were to evaluate the expression and homologous regulation of the GHS-receptor (GHS-R) gene in rat adrenal and to assess the effects of synthetic (GH releasing peptide-6 - GHRP-6) and natural (ghrelin) ligands of GHS-R upon basal and ACTH-stimulated corticosterone secretion in vitro.
Summary · context
Evaluation of corticosterone secretion by incubated adrenal tIssue was carried out by specific RIA.
Report a problemFND-1244490107

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
9 Oct 2026, 03:13 UTC
First added · source update from Europe PMC

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