AMP kinase activation ameliorates insulin resistance induced by free fatty acids in rat skeletal muscle.

Olsen GS, Hansen BF

American journal of physiology. Endocrinology and metabolism1 Nov 2002Cited by 37 (Europe PMC)

PMID12376323DOI10.1152/ajpendo.00118.2002PepBankPUB-12376323

Publisher page PubMedAsk about this study

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study9

7 results from studies that gave the peptide. No result from this study comes from people.

01LabEx vivo tissueMechanism
In isolated rat muscle, AICAR eliminated palmitate inhibition of insulin-stimulated glycogen synthesis.
In plain wordsIn lab tests, AICAR reversed palmitate inhibition of sugar storage in rat muscle compared with palmitate alone.
Peptide
AICAR (acadesine)
Outcome
IncreaseInsulin-stimulated glycogen synthesis
Study
ratn: not reported in source
palmitate-exposed extensor digitorum longus
Comparator
Palmitate without AICAR
Outcome group
Glycemic control · Musculoskeletal
Studied dose
2 mM · last hour of 5-h incubationAs given in this study (rat).
Source sentences
Summary
When including 2 mM AICAR in the last hour of the 5-h incubation with palmitate, the inhibitory effect of palmitate on insulin-stimulated glycogen synthesis and glucose transport was eliminated.
1 context sentence(design, population, supporting numbers)
Summary · context
We examined whether acute activation of 5'-AMP-activated protein kinase (AMPK) by 5'-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside (AICAR) ameliorates insulin resistance in isolated rat skeletal muscle.
Report a problemFND-1237632301
02LabEx vivo tissueMechanism
In isolated rat muscle, AICAR eliminated palmitate inhibition of insulin-stimulated glucose transport.
In plain wordsIn lab tests, AICAR reversed palmitate inhibition of sugar transport in rat muscle compared with palmitate alone.
Peptide
AICAR (acadesine)
Outcome
IncreaseInsulin-stimulated glucose transport
Study
ratn: not reported in source
palmitate-exposed extensor digitorum longus
Comparator
Palmitate without AICAR
Outcome group
Glycemic control · Musculoskeletal
Studied dose
2 mM · last hour of 5-h incubationAs given in this study (rat).
Source sentences
Summary
When including 2 mM AICAR in the last hour of the 5-h incubation with palmitate, the inhibitory effect of palmitate on insulin-stimulated glycogen synthesis and glucose transport was eliminated.
1 context sentence(design, population, supporting numbers)
Summary · context
We examined whether acute activation of 5'-AMP-activated protein kinase (AMPK) by 5'-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside (AICAR) ameliorates insulin resistance in isolated rat skeletal muscle.
Report a problemFND-1237632302
03LabEx vivo tissueMechanism
In isolated rat muscle, AICAR's effect accompanied AMPK activation.
In plain wordsIn lab tests, AICAR activated AMPK in rat muscle.
Peptide
AICAR (acadesine)
Outcome
IncreaseAMPK activation
Study
ratn: not reported in source
palmitate-exposed extensor digitorum longus
Outcome group
Glycemic control · Musculoskeletal
Studied dose
2 mM · last hour of 5-h incubationAs given in this study (rat).
Source sentences
Summary
This effect of AICAR was accompanied by activation of AMPK.
1 context sentence(design, population, supporting numbers)
Summary · context
We examined whether acute activation of 5'-AMP-activated protein kinase (AMPK) by 5'-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside (AICAR) ameliorates insulin resistance in isolated rat skeletal muscle.
Report a problemFND-1237632303
04LabEx vivo tissueMechanism
In isolated rat muscle, AICAR did not affect total glycogen content.
In plain wordsIn lab tests, AICAR did not change total stored sugar in rat muscle.
Peptide
AICAR (acadesine)
Outcome
No significant changeTotal glycogen content
Study
ratn: not reported in source
palmitate-exposed extensor digitorum longus
Outcome group
Glycemic control · Musculoskeletal
Studied dose
2 mM · last hour of 5-h incubationAs given in this study (rat).
Source sentences
Summary
Neither treatment affected total glycogen content.
2 context sentences(design, population, supporting numbers)
Summary · context
When including 2 mM AICAR in the last hour of the 5-h incubation with palmitate, the inhibitory effect of palmitate on insulin-stimulated glycogen synthesis and glucose transport was eliminated.
Summary · context
We examined whether acute activation of 5'-AMP-activated protein kinase (AMPK) by 5'-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside (AICAR) ameliorates insulin resistance in isolated rat skeletal muscle.
Report a problemFND-1237632305
05LabEx vivo tissueMechanism
In isolated rat muscle, AICAR increased glucose 6-phosphate.
In plain wordsIn lab tests, AICAR increased glucose 6-phosphate in rat muscle.
Peptide
AICAR (acadesine)
Outcome
IncreaseGlucose 6-phosphate
Study
ratn: not reported in source
palmitate-exposed extensor digitorum longus
Outcome group
Glycemic control · Musculoskeletal
Studied dose
2 mM · last hour of 5-h incubationAs given in this study (rat).
Source sentences
Summary
However, glucose 6-phosphate was increased after inclusion of AICAR, indicating increased influx of glucose.
1 context sentence(design, population, supporting numbers)
Summary · context
We examined whether acute activation of 5'-AMP-activated protein kinase (AMPK) by 5'-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside (AICAR) ameliorates insulin resistance in isolated rat skeletal muscle.
Report a problemFND-1237632306
06LabEx vivo tissueMechanism
In isolated rat muscle, AICAR did not alter palmitate inhibition of insulin-stimulated glycogen synthase activity.
In plain wordsIn lab tests, AICAR did not reverse palmitate inhibition of glycogen synthase activity in rat muscle.
Peptide
AICAR (acadesine)
Outcome
No significant changeInsulin-stimulated glycogen synthase activity
Study
ratn: not reported in source
palmitate-exposed extensor digitorum longus
Outcome group
Glycemic control · Musculoskeletal
Studied dose
2 mM · last hour of 5-h incubationAs given in this study (rat).
Source sentences
Summary
No effect of AICAR on the inhibited insulin-stimulated GS activity or increased GS phosphorylation by palmitate could be detected.
1 context sentence(design, population, supporting numbers)
Summary · context
We examined whether acute activation of 5'-AMP-activated protein kinase (AMPK) by 5'-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside (AICAR) ameliorates insulin resistance in isolated rat skeletal muscle.
Report a problemFND-1237632307
07LabEx vivo tissueMechanism
In isolated rat muscle, AICAR did not alter the increased glycogen synthase phosphorylation associated with palmitate.
In plain wordsIn lab tests, AICAR did not change palmitate-associated glycogen synthase phosphorylation in rat muscle.
Peptide
AICAR (acadesine)
Outcome
No significant changeGlycogen synthase phosphorylation
Study
ratn: not reported in source
palmitate-exposed extensor digitorum longus
Outcome group
Glycemic control · Musculoskeletal
Studied dose
2 mM · last hour of 5-h incubationAs given in this study (rat).
Source sentences
Summary
No effect of AICAR on the inhibited insulin-stimulated GS activity or increased GS phosphorylation by palmitate could be detected.
1 context sentence(design, population, supporting numbers)
Summary · context
We examined whether acute activation of 5'-AMP-activated protein kinase (AMPK) by 5'-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside (AICAR) ameliorates insulin resistance in isolated rat skeletal muscle.
Report a problemFND-1237632308

Studies that did not test the peptide alone2

In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking AICAR (acadesine).

08LabNot a test of AICAR (acadesine) aloneEx vivo tissueMechanism
In isolated rat muscle, AMPK inhibition prevented AICAR reversal of palmitate inhibition of insulin-stimulated glycogen synthesis.
In plain wordsIn lab tests, blocking AMPK prevented AICAR from reversing palmitate inhibition of glycogen synthesis in rat muscle.
Peptide
AICAR (acadesine)
Outcome
DecreaseAMPK dependence of glycogen synthesis rescue
Study
ratn: not reported in source
palmitate-exposed extensor digitorum longus
Outcome group
Glycemic control · Musculoskeletal
Source sentences
Summary
Importantly, AMPK inhibition was able to prevent this effect.
2 context sentences(design, population, supporting numbers)
Summary · context
When including 2 mM AICAR in the last hour of the 5-h incubation with palmitate, the inhibitory effect of palmitate on insulin-stimulated glycogen synthesis and glucose transport was eliminated.
Summary · context
We examined whether acute activation of 5'-AMP-activated protein kinase (AMPK) by 5'-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside (AICAR) ameliorates insulin resistance in isolated rat skeletal muscle.
In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AICAR (acadesine).
Report a problemFND-1237632304
09LabNot a test of AICAR (acadesine) aloneEx vivo tissueMechanism
In isolated rat muscle, AMPK inhibition prevented AICAR reversal of palmitate inhibition of insulin-stimulated glucose transport.
In plain wordsIn lab tests, blocking AMPK prevented AICAR from reversing palmitate inhibition of glucose transport in rat muscle.
Peptide
AICAR (acadesine)
Outcome
DecreaseAMPK dependence of glucose transport rescue
Study
ratn: not reported in source
palmitate-exposed extensor digitorum longus
Outcome group
Glycemic control · Musculoskeletal
Source sentences
Summary
Importantly, AMPK inhibition was able to prevent this effect.
2 context sentences(design, population, supporting numbers)
Summary · context
When including 2 mM AICAR in the last hour of the 5-h incubation with palmitate, the inhibitory effect of palmitate on insulin-stimulated glycogen synthesis and glucose transport was eliminated.
Summary · context
We examined whether acute activation of 5'-AMP-activated protein kinase (AMPK) by 5'-aminoimidazole-4-carboxamide-1-beta-D-ribonucleoside (AICAR) ameliorates insulin resistance in isolated rat skeletal muscle.
In this study, AICAR (acadesine) was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking AICAR (acadesine).
Report a problemFND-1237632309

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

Publication types

MeSH terms

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