The effect of two-day treatment of primary cultured ovine somatotropes with GHRP-2 on membrane voltage-gated K+ currents.

Chen C

Endocrinology1 Jul 2002Cited by 7 (Europe PMC)

PMID12072399DOI10.1210/endo.143.7.8916PepBankPUB-12072399

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study9

4 results from studies that gave the peptide. No result from this study comes from people.

01LabIn vitro (cells)Mechanism
In cultured ovine somatotropes, 48-h GHRP‑2 treatment increased the percentage of cells with spontaneous action potentials, 51% versus 27%.
In plain wordsIn cultured sheep pituitary cells, two days of GHRP‑2 raised the share of cells that fire spontaneously.
Peptide
GHRP-2
Outcome
IncreaseCells with spontaneous action potential
Study
sheepn: not reported in source
Primary cultured ovine somatotropes, whole-cell patch clamp
Comparator
Untreated cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
10(-8) M · 48 hAs given in this study (sheep).
Measurements
Cells with spontaneous action potential
51%
GHRP-2 48 h
Cells with spontaneous action potential
27%
control
Source sentences
Summary
After 48-h treatment with GHRP-2 (10(-8) M), the percentage of cells with spontaneous action potential was increased (51 vs. 27%) without change of resting potential.
2 context sentences(design, population, supporting numbers)
Summary · context
In this experiment, somatotropes were obtained from ovine pituitaries by enzyme dissociation and enriched by density centrifugation.
Summary · context
Membrane voltage and currents were recorded with whole-cell patch-clamp configuration.
Report a problemFND-1207239901
02LabIn vitro (cells)Mechanism
In cultured ovine somatotropes, 48-h GHRP‑2 treatment did not change the resting potential.
In plain wordsIn cultured sheep pituitary cells, two days of GHRP‑2 did not change the resting voltage.
Peptide
GHRP-2
Outcome
No significant changeResting potential
Study
sheepn: not reported in source
Primary cultured ovine somatotropes, whole-cell patch clamp
Comparator
Untreated cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
10(-8) M · 48 hAs given in this study (sheep).
Source sentences
Summary
without change of resting potential.
2 context sentences(design, population, supporting numbers)
Summary · context
In this experiment, somatotropes were obtained from ovine pituitaries by enzyme dissociation and enriched by density centrifugation.
Summary · context
Membrane voltage and currents were recorded with whole-cell patch-clamp configuration.
Report a problemFND-1207239902
03LabIn vitro (cells)Mechanism
In cultured ovine somatotropes, 48-h GHRP‑2 treatment increased the amplitude of voltage-gated K+ currents, mainly the transient A-type-like current.
In plain wordsIn cultured sheep pituitary cells, two days of GHRP‑2 increased potassium currents.
Peptide
GHRP-2
Outcome
IncreaseAmplitude of voltage-gated K+ currents
Study
sheepn: not reported in source
Primary cultured ovine somatotropes, whole-cell patch clamp
Comparator
Untreated cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
10(-8) M · 48 hAs given in this study (sheep).
Source sentences
Summary
This GHRP-2 treatment also increased the amplitude of voltage-gated K+ currents (predominantly transient A-type-like current but also delayed rectifier or K-type-like current) without modification of biophysical kinetics.
2 context sentences(design, population, supporting numbers)
Summary · context
In this experiment, somatotropes were obtained from ovine pituitaries by enzyme dissociation and enriched by density centrifugation.
Summary · context
Membrane voltage and currents were recorded with whole-cell patch-clamp configuration.
Report a problemFND-1207239903
04LabIn vitro (cells)Mechanism
In cultured ovine somatotropes, 48-h GHRP‑2 treatment did not modify the biophysical kinetics of K+ currents.
In plain wordsIn cultured sheep pituitary cells, two days of GHRP‑2 did not change how the potassium currents behave over time.
Peptide
GHRP-2
Outcome
No significant changeBiophysical kinetics of K+ currents
Study
sheepn: not reported in source
Primary cultured ovine somatotropes, whole-cell patch clamp
Comparator
Untreated cells
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Studied dose
10(-8) M · 48 hAs given in this study (sheep).
Source sentences
Summary
without modification of biophysical kinetics.
2 context sentences(design, population, supporting numbers)
Summary · context
In this experiment, somatotropes were obtained from ovine pituitaries by enzyme dissociation and enriched by density centrifugation.
Summary · context
Membrane voltage and currents were recorded with whole-cell patch-clamp configuration.
Report a problemFND-1207239904

Studies that did not test the peptide alone5

In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-2.

05LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In cultured ovine somatotropes, down-regulation of PKC with phorbol 12-myristate 13-acetate blocked the GHRP‑2-induced increase in K+ currents.
In plain wordsIn cultured sheep pituitary cells, switching off PKC blocked the potassium current increase caused by GHRP‑2.
Peptide
GHRP-2
Outcome
DecreaseGHRP-2-induced K+ current increase after PKC down-regulation
Study
sheepn: not reported in source
Primary cultured ovine somatotropes, whole-cell patch clamp
Comparator
GHRP-2 without PMA
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Down-regulation of protein kinase C (PKC) with phorbol 12-myristate 13-acetate at the time of adding GHRP-2 blocked the increase in K+ currents.
2 context sentences(design, population, supporting numbers)
Summary · context
In this experiment, somatotropes were obtained from ovine pituitaries by enzyme dissociation and enriched by density centrifugation.
Summary · context
Membrane voltage and currents were recorded with whole-cell patch-clamp configuration.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1207239905
06LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In cultured ovine somatotropes, the PKC inhibitor calphostin C significantly reduced the GHRP‑2-induced increase in K+ currents.
In plain wordsIn cultured sheep pituitary cells, a PKC blocker reduced the potassium current increase caused by GHRP‑2.
Peptide
GHRP-2
Outcome
DecreaseGHRP-2-induced K+ current increase with calphostin C
Study
sheepn: not reported in source
Primary cultured ovine somatotropes, whole-cell patch clamp
Comparator
GHRP-2 without inhibitor
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Inclusion of calphostin C (PKC inhibitor) but not H(89) (protein kinase A inhibitor) significantly reduced the increase in K+ currents by GHRP-2.
2 context sentences(design, population, supporting numbers)
Summary · context
In this experiment, somatotropes were obtained from ovine pituitaries by enzyme dissociation and enriched by density centrifugation.
Summary · context
Membrane voltage and currents were recorded with whole-cell patch-clamp configuration.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1207239906
07LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In cultured ovine somatotropes, the protein kinase A inhibitor H89 did not significantly reduce the GHRP‑2-induced increase in K+ currents.
In plain wordsIn cultured sheep pituitary cells, a protein kinase A blocker did not reduce the potassium current increase caused by GHRP‑2.
Peptide
GHRP-2
Outcome
No significant changeGHRP-2-induced K+ current increase with H89
Study
sheepn: not reported in source
Primary cultured ovine somatotropes, whole-cell patch clamp
Comparator
GHRP-2 without inhibitor
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Inclusion of calphostin C (PKC inhibitor) but not H(89) (protein kinase A inhibitor) significantly reduced the increase in K+ currents by GHRP-2.
2 context sentences(design, population, supporting numbers)
Summary · context
In this experiment, somatotropes were obtained from ovine pituitaries by enzyme dissociation and enriched by density centrifugation.
Summary · context
Membrane voltage and currents were recorded with whole-cell patch-clamp configuration.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1207239907
08LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In cultured ovine somatotropes, the transcription inhibitor actinomycin D abolished the GHRP‑2-induced increase in K+ currents.
In plain wordsIn cultured sheep pituitary cells, blocking gene transcription stopped the potassium current increase caused by GHRP‑2.
Peptide
GHRP-2
Outcome
DecreaseGHRP-2-induced K+ current increase with actinomycin D
Study
sheepn: not reported in source
Primary cultured ovine somatotropes, whole-cell patch clamp
Comparator
GHRP-2 without inhibitor
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Inclusion of actinomycin D (transcription inhibitor) or cycloheximide (protein synthesis inhibitor) abolished the increase in K+ currents.
2 context sentences(design, population, supporting numbers)
Summary · context
In this experiment, somatotropes were obtained from ovine pituitaries by enzyme dissociation and enriched by density centrifugation.
Summary · context
Membrane voltage and currents were recorded with whole-cell patch-clamp configuration.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1207239908
09LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism
In cultured ovine somatotropes, the protein synthesis inhibitor cycloheximide abolished the GHRP‑2-induced increase in K+ currents.
In plain wordsIn cultured sheep pituitary cells, blocking protein synthesis stopped the potassium current increase caused by GHRP‑2.
Peptide
GHRP-2
Outcome
DecreaseGHRP-2-induced K+ current increase with cycloheximide
Study
sheepn: not reported in source
Primary cultured ovine somatotropes, whole-cell patch clamp
Comparator
GHRP-2 without inhibitor
Outcome group
Receptor activity and mechanism · Endocrine and hormones
Source sentences
Summary
Inclusion of actinomycin D (transcription inhibitor) or cycloheximide (protein synthesis inhibitor) abolished the increase in K+ currents.
2 context sentences(design, population, supporting numbers)
Summary · context
In this experiment, somatotropes were obtained from ovine pituitaries by enzyme dissociation and enriched by density centrifugation.
Summary · context
Membrane voltage and currents were recorded with whole-cell patch-clamp configuration.
In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.
Report a problemFND-1207239909

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
13 Oct 2026, 21:44 UTC
Study results updated
Version 1
13 Oct 2026, 16:31 UTC
First added · source update from Europe PMC

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