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Study · Not a test of the peptide alone · 2002

3 results

Detection of oxytocin mRNA in hypertonic saline Fos-activated PVN neurons: comparison of chromogens in dual immunocytochemical and in situ hybridization procedure.

Pirnik Z, Kiss A

Endocrine regulations1 Mar 2002Cited by 6 (Europe PMC)

PMID11971748 (opens in a new tab)PepBankPUB-11971748
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone3

In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Oxytocin.

  1. 01LabNot a test of Oxytocin aloneEx vivo tissueOther

    Fos-DAB labeling combined with oxytocin mRNA in situ hybridization gave good staining quality on fresh and 24-month stored mounted sections.

    In plain wordsIn lab tests on brain sections, a staining method for oxytocin messenger RNA worked well on fresh sections and sections stored for 24 months.

    Peptide
    Oxytocin
    Outcome
    Not applicableStaining quality of Fos-DAB with oxytocin mRNA in situ hybridization
    Study

    n: not reported in source

    Hypothalamic sections, dual Fos immunostaining and oxytocin mRNA in situ hybridization
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition

    Source sentences

    Summary
    The first staining sequence with Fos-DAB labeling resulted in a good staining quality on both the fresh and for 24 month stored mounted sections.
    1 context sentence
    Summary · context
    Two dual labeling procedures were compared: 1/ Fos-immunostaining with DAB alone and combined with OXY mRNA in situ hybridization and 2/ Fos-immunostaining with DAB-Ni-Co and combined with OXY mRNA in situ hybridization.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  2. 02LabNot a test of Oxytocin aloneEx vivo tissueOther

    Free floating sections were fragile at the end of the hybridization procedure and were difficult to mount and stretch.

    In plain wordsIn lab tests, free floating brain sections broke easily and were hard to mount after the staining procedure.

    Peptide
    Oxytocin
    Outcome
    Not applicableFragility of free floating sections
    Study

    n: not reported in source

    Hypothalamic sections, dual Fos immunostaining and oxytocin mRNA in situ hybridization
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition

    Source sentences

    Summary
    Although the free floating sections during the in situ procedure showed the same staining properties as the mounted ones, with respect to their increased fragility on the end of the hybridization procedure, they were difficult to mount and stretch on poly-L-lysine coated slides.
    1 context sentence
    Summary · context
    Two dual labeling procedures were compared: 1/ Fos-immunostaining with DAB alone and combined with OXY mRNA in situ hybridization and 2/ Fos-immunostaining with DAB-Ni-Co and combined with OXY mRNA in situ hybridization.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  3. 03LabNot a test of Oxytocin aloneEx vivo tissueOther

    The DAB-Ni-Co chromogen complex was unstable and disappeared completely from all section types after the in situ hybridization process.

    In plain wordsIn lab tests, the nickel and cobalt stain faded away completely after the oxytocin messenger RNA step.

    Peptide
    Oxytocin
    Outcome
    Not applicableStability of DAB-Ni-Co after in situ hybridization
    Study

    n: not reported in source

    Hypothalamic sections, dual Fos immunostaining and oxytocin mRNA in situ hybridization
    Outcome group
    Receptor activity and mechanism · Nervous system and cognition

    Source sentences

    Summary
    However, DAB-Ni-Co mixture showed up as an unstable chromogen complex, which after completing the in situ hybridization process completely disappeared from each type of section.
    1 context sentence
    Summary · context
    Two dual labeling procedures were compared: 1/ Fos-immunostaining with DAB alone and combined with OXY mRNA in situ hybridization and 2/ Fos-immunostaining with DAB-Ni-Co and combined with OXY mRNA in situ hybridization.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    11 Oct 2026, 20:03 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:52 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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