Skip to content

Study · Not a test of the peptide alone · 2001

3 results

Quantitative in situ hybridization for peptide mRNAs in mouse brain.

Key M, Wirick B, Cool D, Morris M

Brain research. Brain research protocols1 Aug 2001Cited by 17 (Europe PMC)

PMID11522523 (opens in a new tab)DOI10.1016/s1385-299x(01)00068-x (opens in a new tab)PepBankPUB-11522523
Publisher pagePubMedAsk about this study
Jump to 3 results

Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone3

In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking Oxytocin.

  1. 01LabNot a test of Oxytocin aloneEx vivo tissueOther

    In mouse brain sections, in situ hybridization gave an intense signal for vasopressin and oxytocin mRNA in the paraventricular and supraoptic nuclei.

    In plain wordsIn mouse brain slices, the test gave a strong signal for oxytocin and vasopressin messenger RNA in two brain regions.

    Peptide
    Oxytocin
    Outcome
    Not applicableHybridization signal in PVN and SON
    Study

    mousen: not reported in source

    Mouse brain sections, in situ hybridization
    Outcome group
    Analytical methods and detection · Nervous system and cognition

    Source sentences

    Summary
    Results showed that there was an intense hybridization reaction in the PVN and SON, making it possible to distinguish the specific brain regions.
    1 context sentence
    Summary · context
    Mouse brains were fixed in 4% paraformaldehyde (PFA) and processed for in situ hybridization using 35S-oligonucleotide probes for VP and OT.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  2. 02LabNot a test of Oxytocin aloneEx vivo tissueOther

    In mouse brain sections, the mRNA signal could be measured after as little as 4 hours of exposure.

    In plain wordsIn mouse brain slices, the signal could be measured after only 4 hours of exposure.

    Peptide
    Oxytocin
    Outcome
    Not applicableMinimum exposure time for signal measurement
    Study

    mousen: not reported in source

    Mouse brain sections, in situ hybridization
    Outcome group
    Analytical methods and detection · Nervous system and cognition

    Source sentences

    Summary
    A comparison of the different exposure times showed that the signal could be measured after as little as 4 h.
    1 context sentence
    Summary · context
    Mouse brains were fixed in 4% paraformaldehyde (PFA) and processed for in situ hybridization using 35S-oligonucleotide probes for VP and OT.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

  3. 03LabNot a test of Oxytocin aloneEx vivo tissueOther

    In mouse brain sections, the free-floating method gave lower background and a higher signal than the slide-based system.

    In plain wordsIn mouse brain slices, free-floating sections gave a cleaner and stronger signal than slide-mounted ones.

    Peptide
    Oxytocin
    Outcome
    Not applicableFree-floating versus slide-mounted sections
    Study

    mousen: not reported in source

    Mouse brain sections, in situ hybridization
    Comparator
    Slide-mounted sections
    Outcome group
    Analytical methods and detection · Nervous system and cognition

    Source sentences

    Summary
    The free-floating method was superior to the slide-based system, providing a lower background and a higher signal.
    1 context sentence
    Summary · context
    Mouse brains were fixed in 4% paraformaldehyde (PFA) and processed for in situ hybridization using 35S-oligonucleotide probes for VP and OT.

    In this study, Oxytocin was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking Oxytocin.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    11 Oct 2026, 20:03 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 02:53 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

PepBank is a research reference. Talk to a doctor before you use any peptide.