Effect of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside infusion on in vivo glucose and lipid metabolism in lean and obese Zucker rats.

Bergeron R, Previs SF, Cline GW, Perret P, Russell RR 3rd, Young LH, Shulman GI

Diabetes1 May 2001Cited by 219 (Europe PMC)

PMID11334411DOI10.2337/diabetes.50.5.1076PepBankPUB-11334411

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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study12

12 results from studies that gave the peptide. No result from this study comes from people.

01AnimalAnimal study (in vivo)Mechanism
In lean and obese Zucker rats, AICAR increased muscle AMPK activity more than fivefold versus control.
In plain wordsIn rats, AICAR increased muscle AMPK activity compared with control.
Peptide
AICAR (acadesine)
Outcome
IncreaseMuscle AMPK activity
Study
ratn: not reported in source
lean and obese Zucker rats
Comparator
Control
Outcome group
Receptor activity and mechanism · Metabolic and weight
Studied dose
bolus 100 mg/kg; constant 10 mg.kg(-1).min(-1) · infusion · 90 minAs given in this study (rat).
Measurements
AMPK activity
> 5 fold
lean and obese rats
Source sentences
Summary
AICAR infusion increased muscle AMPK activity more than fivefold (P < 0.01 vs. control and insulin) in both lean and obese rats.
2 context sentences(design, population, supporting numbers)
Summary · context
To examine the effects of AMPK activation on skeletal muscle glucose transport activity and whole-body carbohydrate and lipid metabolism in an insulin-resistant rat model, awake obese Zuckerfa/fa rats (n = 26) and their lean (n = 23) littermates were infused for 90 min with AICAR, insulin, or saline.
Summary · context
The insulin infusion rate (4 mU.kg(-1).min(-1)) was selected to match the glucose requirements during AICAR (bolus, 100 mg/kg; constant, 10 mg.kg(-1).min(-1)) isoglycemic clamps in the lean rats.
Report a problemFND-1133441101
02AnimalAnimal study (in vivo)Mechanism
In lean and obese Zucker rats, AICAR increased muscle AMPK activity more than fivefold versus insulin.
In plain wordsIn rats, AICAR increased muscle AMPK activity compared with insulin.
Peptide
AICAR (acadesine)
Outcome
IncreaseMuscle AMPK activity
Study
ratn: not reported in source
lean and obese Zucker rats
Comparator
Insulin
Outcome group
Receptor activity and mechanism · Metabolic and weight
Studied dose
bolus 100 mg/kg; constant 10 mg.kg(-1).min(-1) · infusion · 90 minAs given in this study (rat).
Measurements
AMPK activity
> 5 fold
lean and obese rats
Source sentences
Summary
AICAR infusion increased muscle AMPK activity more than fivefold (P < 0.01 vs. control and insulin) in both lean and obese rats.
2 context sentences(design, population, supporting numbers)
Summary · context
To examine the effects of AMPK activation on skeletal muscle glucose transport activity and whole-body carbohydrate and lipid metabolism in an insulin-resistant rat model, awake obese Zuckerfa/fa rats (n = 26) and their lean (n = 23) littermates were infused for 90 min with AICAR, insulin, or saline.
Summary · context
The insulin infusion rate (4 mU.kg(-1).min(-1)) was selected to match the glucose requirements during AICAR (bolus, 100 mg/kg; constant, 10 mg.kg(-1).min(-1)) isoglycemic clamps in the lean rats.
Report a problemFND-1133441102
03AnimalAnimal study (in vivo)Efficacy outcome
In lean and obese Zucker rats, AICAR decreased plasma triglycerides versus basal values.
In plain wordsIn rats, AICAR reduced blood triglycerides from starting values.
Peptide
AICAR (acadesine)
Outcome
DecreasePlasma triglycerides
Study
ratn: not reported in source
lean and obese Zucker rats
Comparator
Basal
Outcome group
Lipids · Metabolic and weight
Studied dose
bolus 100 mg/kg; constant 10 mg.kg(-1).min(-1) · infusion · 90 minAs given in this study (rat).
Source sentences
Summary
Plasma triglycerides, fatty acid concentrations, and glycerol turnover, as assessed by [2-13C]glycerol, were all decreased in both lean and obese rats infused with AICAR (P < 0.05 vs. basal), whereas insulin had no effect on these parameters in the obese rats.
2 context sentences(design, population, supporting numbers)
Summary · context
To examine the effects of AMPK activation on skeletal muscle glucose transport activity and whole-body carbohydrate and lipid metabolism in an insulin-resistant rat model, awake obese Zuckerfa/fa rats (n = 26) and their lean (n = 23) littermates were infused for 90 min with AICAR, insulin, or saline.
Summary · context
The insulin infusion rate (4 mU.kg(-1).min(-1)) was selected to match the glucose requirements during AICAR (bolus, 100 mg/kg; constant, 10 mg.kg(-1).min(-1)) isoglycemic clamps in the lean rats.
Report a problemFND-1133441103
04AnimalAnimal study (in vivo)Efficacy outcome
In lean and obese Zucker rats, AICAR decreased plasma fatty acid concentrations versus basal values.
In plain wordsIn rats, AICAR reduced blood fatty acids from starting values.
Peptide
AICAR (acadesine)
Outcome
DecreasePlasma fatty acids
Study
ratn: not reported in source
lean and obese Zucker rats
Comparator
Basal
Outcome group
Lipids · Metabolic and weight
Studied dose
bolus 100 mg/kg; constant 10 mg.kg(-1).min(-1) · infusion · 90 minAs given in this study (rat).
Source sentences
Summary
Plasma triglycerides, fatty acid concentrations, and glycerol turnover, as assessed by [2-13C]glycerol, were all decreased in both lean and obese rats infused with AICAR (P < 0.05 vs. basal), whereas insulin had no effect on these parameters in the obese rats.
2 context sentences(design, population, supporting numbers)
Summary · context
To examine the effects of AMPK activation on skeletal muscle glucose transport activity and whole-body carbohydrate and lipid metabolism in an insulin-resistant rat model, awake obese Zuckerfa/fa rats (n = 26) and their lean (n = 23) littermates were infused for 90 min with AICAR, insulin, or saline.
Summary · context
The insulin infusion rate (4 mU.kg(-1).min(-1)) was selected to match the glucose requirements during AICAR (bolus, 100 mg/kg; constant, 10 mg.kg(-1).min(-1)) isoglycemic clamps in the lean rats.
Report a problemFND-1133441104
05AnimalAnimal study (in vivo)Efficacy outcome
In lean and obese Zucker rats, AICAR decreased glycerol turnover versus basal values.
In plain wordsIn rats, AICAR reduced glycerol turnover from starting values.
Peptide
AICAR (acadesine)
Outcome
DecreaseGlycerol turnover
Study
ratn: not reported in source
lean and obese Zucker rats
Comparator
Basal
Outcome group
Lipids · Metabolic and weight
Studied dose
bolus 100 mg/kg; constant 10 mg.kg(-1).min(-1) · infusion · 90 minAs given in this study (rat).
Source sentences
Summary
Plasma triglycerides, fatty acid concentrations, and glycerol turnover, as assessed by [2-13C]glycerol, were all decreased in both lean and obese rats infused with AICAR (P < 0.05 vs. basal), whereas insulin had no effect on these parameters in the obese rats.
2 context sentences(design, population, supporting numbers)
Summary · context
To examine the effects of AMPK activation on skeletal muscle glucose transport activity and whole-body carbohydrate and lipid metabolism in an insulin-resistant rat model, awake obese Zuckerfa/fa rats (n = 26) and their lean (n = 23) littermates were infused for 90 min with AICAR, insulin, or saline.
Summary · context
The insulin infusion rate (4 mU.kg(-1).min(-1)) was selected to match the glucose requirements during AICAR (bolus, 100 mg/kg; constant, 10 mg.kg(-1).min(-1)) isoglycemic clamps in the lean rats.
Report a problemFND-1133441105
06AnimalAnimal study (in vivo)Efficacy outcome
In lean and obese Zucker rats, AICAR suppressed endogenous glucose production by more than 50% versus basal values.
In plain wordsIn rats, AICAR reduced the body's glucose production from starting values.
Peptide
AICAR (acadesine)
Outcome
DecreaseEndogenous glucose production
Study
ratn: not reported in source
lean and obese Zucker rats
Comparator
Basal
Outcome group
Glycemic control · Metabolic and weight
Studied dose
bolus 100 mg/kg; constant 10 mg.kg(-1).min(-1) · infusion · 90 minAs given in this study (rat).
Measurements
Glucose production suppression
> 50%
lean and obese rats
Source sentences
Summary
Endogenous glucose production rates, measured by [U-13C]glucose, were suppressed by >50% during AICAR and insulin infusions in both lean and obese rats (P < 0.05 vs. basal).
2 context sentences(design, population, supporting numbers)
Summary · context
To examine the effects of AMPK activation on skeletal muscle glucose transport activity and whole-body carbohydrate and lipid metabolism in an insulin-resistant rat model, awake obese Zuckerfa/fa rats (n = 26) and their lean (n = 23) littermates were infused for 90 min with AICAR, insulin, or saline.
Summary · context
The insulin infusion rate (4 mU.kg(-1).min(-1)) was selected to match the glucose requirements during AICAR (bolus, 100 mg/kg; constant, 10 mg.kg(-1).min(-1)) isoglycemic clamps in the lean rats.
Report a problemFND-1133441106
07AnimalAnimal study (in vivo)Efficacy outcome
In lean Zucker rats, AICAR increased whole-body glucose disposal more than twofold versus basal values.
In plain wordsIn lean rats, AICAR increased whole-body sugar disposal from starting values.
Peptide
AICAR (acadesine)
Outcome
IncreaseWhole-body glucose disposal in lean rats
Study
ratn: not reported in source
lean Zucker rats
Comparator
Basal
Outcome group
Glycemic control · Metabolic and weight
Studied dose
bolus 100 mg/kg; constant 10 mg.kg(-1).min(-1) · infusion · 90 minAs given in this study (rat).
Measurements
Glucose disposal increase
> 2 fold
lean rats
Source sentences
Summary
In lean rats, rates of whole-body glucose disposal increased by more than two-fold (P < 0.05 vs. basal) during both AICAR and insulin infusion;
2 context sentences(design, population, supporting numbers)
Summary · context
To examine the effects of AMPK activation on skeletal muscle glucose transport activity and whole-body carbohydrate and lipid metabolism in an insulin-resistant rat model, awake obese Zuckerfa/fa rats (n = 26) and their lean (n = 23) littermates were infused for 90 min with AICAR, insulin, or saline.
Summary · context
The insulin infusion rate (4 mU.kg(-1).min(-1)) was selected to match the glucose requirements during AICAR (bolus, 100 mg/kg; constant, 10 mg.kg(-1).min(-1)) isoglycemic clamps in the lean rats.
Report a problemFND-1133441107
08AnimalAnimal study (in vivo)Efficacy outcome
In lean Zucker rats, AICAR increased soleus glucose transport more than 2.2-fold versus control.
In plain wordsIn lean rats, AICAR increased soleus muscle sugar transport compared with control.
Peptide
AICAR (acadesine)
Outcome
IncreaseSoleus glucose transport in lean rats
Study
ratn: not reported in source
lean Zucker rats
Comparator
Control
Outcome group
Glycemic control · Metabolic and weight
Studied dose
bolus 100 mg/kg; constant 10 mg.kg(-1).min(-1) · infusion · 90 minAs given in this study (rat).
Measurements
Glucose transport increase
> 2.2 fold
soleus, lean rats
Source sentences
Summary
[3H]2-deoxy-D-glucose transport activity increased to a similar extent, by >2.2-fold (both P < 0.05 vs. control), in both soleus and red gastrocnemius muscles of lean rats infused with either AICAR or insulin.
2 context sentences(design, population, supporting numbers)
Summary · context
To examine the effects of AMPK activation on skeletal muscle glucose transport activity and whole-body carbohydrate and lipid metabolism in an insulin-resistant rat model, awake obese Zuckerfa/fa rats (n = 26) and their lean (n = 23) littermates were infused for 90 min with AICAR, insulin, or saline.
Summary · context
The insulin infusion rate (4 mU.kg(-1).min(-1)) was selected to match the glucose requirements during AICAR (bolus, 100 mg/kg; constant, 10 mg.kg(-1).min(-1)) isoglycemic clamps in the lean rats.
Report a problemFND-1133441108
09AnimalAnimal study (in vivo)Efficacy outcome
In lean Zucker rats, AICAR increased red gastrocnemius glucose transport more than 2.2-fold versus control.
In plain wordsIn lean rats, AICAR increased red gastrocnemius muscle sugar transport compared with control.
Peptide
AICAR (acadesine)
Outcome
IncreaseRed gastrocnemius glucose transport in lean rats
Study
ratn: not reported in source
lean Zucker rats
Comparator
Control
Outcome group
Glycemic control · Metabolic and weight
Studied dose
bolus 100 mg/kg; constant 10 mg.kg(-1).min(-1) · infusion · 90 minAs given in this study (rat).
Measurements
Glucose transport increase
> 2.2 fold
red gastrocnemius, lean rats
Source sentences
Summary
[3H]2-deoxy-D-glucose transport activity increased to a similar extent, by >2.2-fold (both P < 0.05 vs. control), in both soleus and red gastrocnemius muscles of lean rats infused with either AICAR or insulin.
2 context sentences(design, population, supporting numbers)
Summary · context
To examine the effects of AMPK activation on skeletal muscle glucose transport activity and whole-body carbohydrate and lipid metabolism in an insulin-resistant rat model, awake obese Zuckerfa/fa rats (n = 26) and their lean (n = 23) littermates were infused for 90 min with AICAR, insulin, or saline.
Summary · context
The insulin infusion rate (4 mU.kg(-1).min(-1)) was selected to match the glucose requirements during AICAR (bolus, 100 mg/kg; constant, 10 mg.kg(-1).min(-1)) isoglycemic clamps in the lean rats.
Report a problemFND-1133441109
10AnimalAnimal study (in vivo)Efficacy outcome
In obese Zucker rats, AICAR did not stimulate whole-body glucose disposal.
In plain wordsIn obese rats, AICAR did not increase whole-body sugar disposal.
Peptide
AICAR (acadesine)
Outcome
No significant changeWhole-body glucose disposal in obese rats
Study
ratn: not reported in source
obese Zucker rats
Outcome group
Glycemic control · Metabolic and weight
Studied dose
bolus 100 mg/kg; constant 10 mg.kg(-1).min(-1) · infusion · 90 minAs given in this study (rat).
Source sentences
Summary
In the obese Zucker rats, neither AICAR nor insulin stimulated whole-body glucose disposal or soleus muscle glucose transport activity.
2 context sentences(design, population, supporting numbers)
Summary · context
To examine the effects of AMPK activation on skeletal muscle glucose transport activity and whole-body carbohydrate and lipid metabolism in an insulin-resistant rat model, awake obese Zuckerfa/fa rats (n = 26) and their lean (n = 23) littermates were infused for 90 min with AICAR, insulin, or saline.
Summary · context
The insulin infusion rate (4 mU.kg(-1).min(-1)) was selected to match the glucose requirements during AICAR (bolus, 100 mg/kg; constant, 10 mg.kg(-1).min(-1)) isoglycemic clamps in the lean rats.
Report a problemFND-1133441110
11AnimalAnimal study (in vivo)Efficacy outcome
In obese Zucker rats, AICAR did not stimulate soleus glucose transport.
In plain wordsIn obese rats, AICAR did not increase soleus muscle sugar transport.
Peptide
AICAR (acadesine)
Outcome
No significant changeSoleus glucose transport in obese rats
Study
ratn: not reported in source
obese Zucker rats
Outcome group
Glycemic control · Metabolic and weight
Studied dose
bolus 100 mg/kg; constant 10 mg.kg(-1).min(-1) · infusion · 90 minAs given in this study (rat).
Source sentences
Summary
In the obese Zucker rats, neither AICAR nor insulin stimulated whole-body glucose disposal or soleus muscle glucose transport activity.
2 context sentences(design, population, supporting numbers)
Summary · context
To examine the effects of AMPK activation on skeletal muscle glucose transport activity and whole-body carbohydrate and lipid metabolism in an insulin-resistant rat model, awake obese Zuckerfa/fa rats (n = 26) and their lean (n = 23) littermates were infused for 90 min with AICAR, insulin, or saline.
Summary · context
The insulin infusion rate (4 mU.kg(-1).min(-1)) was selected to match the glucose requirements during AICAR (bolus, 100 mg/kg; constant, 10 mg.kg(-1).min(-1)) isoglycemic clamps in the lean rats.
Report a problemFND-1133441111
12AnimalAnimal study (in vivo)Efficacy outcome
In obese Zucker rats, AICAR increased red gastrocnemius glucose transport approximately 2.4-fold versus control.
In plain wordsIn obese rats, AICAR increased red gastrocnemius muscle sugar transport compared with control.
Peptide
AICAR (acadesine)
Outcome
IncreaseRed gastrocnemius glucose transport in obese rats
Study
ratn: not reported in source
obese Zucker rats
Comparator
Control
Outcome group
Glycemic control · Metabolic and weight
Studied dose
bolus 100 mg/kg; constant 10 mg.kg(-1).min(-1) · infusion · 90 minAs given in this study (rat).
Measurements
Glucose transport increase
≈ 2.4 fold
red gastrocnemius, obese rats
Source sentences
Summary
However, AICAR increased glucose transport activity by approximately 2.4-fold (P < 0.05 vs. control) in the red gastrocnemius from obese rats, whereas insulin had no effect.
2 context sentences(design, population, supporting numbers)
Summary · context
To examine the effects of AMPK activation on skeletal muscle glucose transport activity and whole-body carbohydrate and lipid metabolism in an insulin-resistant rat model, awake obese Zuckerfa/fa rats (n = 26) and their lean (n = 23) littermates were infused for 90 min with AICAR, insulin, or saline.
Summary · context
The insulin infusion rate (4 mU.kg(-1).min(-1)) was selected to match the glucose requirements during AICAR (bolus, 100 mg/kg; constant, 10 mg.kg(-1).min(-1)) isoglycemic clamps in the lean rats.
Report a problemFND-1133441112

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Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

Version 2Current
21 Oct 2026, 15:58 UTC
Study results updated
Version 1
21 Oct 2026, 12:50 UTC
First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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