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Study · Not a test of the peptide alone · 2001

3 results

The in vitro effect of leptin on growth hormone secretion from primary cultured ovine somatotrophs.

Chen C, Roh SG, Nie GY, Loneragan K, Xu RW, Ruan M, Clarke LJ, Goding JW, Gertler A

Endocrine1 Feb 2001Cited by 19 (Europe PMC)

PMID11322504 (opens in a new tab)DOI10.1385/endo:14:1:073 (opens in a new tab)PepBankPUB-11322504
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study3

No result from this study comes from a test of the peptide.

Studies that did not test the peptide alone3

In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. These results are not evidence about taking GHRP-2.

  1. 01LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism

    In cultured ovine somatotrophs, 30 min of leptin treatment did not affect GH secretion stimulated by GHRP‑2.

    In plain wordsIn sheep pituitary cells, a short leptin exposure did not change the growth hormone release caused by GHRP‑2.

    Peptide
    GHRP-2
    Outcome
    No significant changeGHRP-2-stimulated GH secretion after 30 min leptin
    Study

    sheepn: not reported in source

    Primary cultured ovine somatotrophs
    Comparator
    No leptin
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    Treatment of primary cultured ovine somatotrophs with leptin (10(-9)-10(-7) M) for 30 min did not affect basal, GH-releasing hormone (GHRH) (10(-7) M)- or GH-releasing peptide-2 (GHRP-2)(10(-7) M)-stimulated GH secretion.
    2 context sentences
    Summary · context
    Using primary cultured ovine pituitary cells, we studied the direct effect of leptin on the secretion of GH.
    Summary · context
    Pituitary cells were dissociated by collagenase and subjected to Percoll gradient centrifugation to enrich the somatotroph population to 60-80% of cells.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

  2. 02LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism

    In cultured ovine somatotrophs, 1-3 days of leptin treatment increased GH secretion stimulated by GHRP‑2.

    In plain wordsIn sheep pituitary cells, one to three days of leptin raised the growth hormone release caused by GHRP‑2.

    Peptide
    GHRP-2
    Outcome
    IncreaseGHRP-2-stimulated GH secretion after 1-3 days leptin
    Study

    sheepn: not reported in source

    Primary cultured ovine somatotrophs
    Comparator
    No leptin
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    Following treatment of cells for 1-3 d with leptin, GHRH-stimulated GH secretion was reduced and GHRP-2-stimulated GH secretion increased.
    2 context sentences
    Summary · context
    Using primary cultured ovine pituitary cells, we studied the direct effect of leptin on the secretion of GH.
    Summary · context
    Pituitary cells were dissociated by collagenase and subjected to Percoll gradient centrifugation to enrich the somatotroph population to 60-80% of cells.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

  3. 03LabNot a test of GHRP-2 aloneIn vitro (cells)Mechanism

    In cultured ovine somatotrophs, 3 days of leptin treatment did not alter the combined effect of GHRH and GHRP‑2 on GH secretion.

    In plain wordsIn sheep pituitary cells, three days of leptin did not change the growth hormone release caused by GHRH and GHRP‑2 together.

    Peptide
    GHRP-2
    Outcome
    No significant changeGHRH plus GHRP-2 combined GH secretion after 3 days leptin
    Study

    sheepn: not reported in source

    Primary cultured ovine somatotrophs
    Comparator
    No leptin
    Outcome group
    Growth hormone and IGF-1 · Endocrine and hormones

    Source sentences

    Summary
    The combined effect of GHRH and GHRP-2 on GH secretion was not altered by the treatment of cells with leptin for 3 d.
    2 context sentences
    Summary · context
    Using primary cultured ovine pituitary cells, we studied the direct effect of leptin on the secretion of GH.
    Summary · context
    Pituitary cells were dissociated by collagenase and subjected to Percoll gradient centrifugation to enrich the somatotroph population to 60-80% of cells.

    In this study, GHRP-2 was a lab tool or a carrier, part of a mix, or the subject of a lab method. This result is not evidence about taking GHRP-2.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    13 Oct 2026, 21:44 UTC

    Study results updated

  2. Version 1

    13 Oct 2026, 16:31 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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