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Study · In vitro (cells) · 2001

2 results

Stimulation of intracellular free calcium in GH3 cells by gamma3-melanocyte-stimulating hormone. Involvement of a novel melanocortin receptor?

Langouche L, Roudbaraki M, Pals K, Denef C

Endocrinology1 Jan 2001Cited by 12 (Europe PMC)

PMID11145589 (opens in a new tab)DOI10.1210/endo.142.1.7878 (opens in a new tab)PepBankPUB-11145589
Publisher pagePubMedAsk about this study
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Summary

The text of this summary is not shown here, because the article license does not allow reuse. Read it at the source (opens in a new tab)

Text rights: Publisher copyright. Results still quote short attributed sentences.

Results from this study2

2 results from studies that gave the peptide. No result from this study comes from people.

  1. 01LabIn vitro (cells)Activity measurement

    In GH3 cells, MTII showed low potency in increasing intracellular free calcium.

    In plain wordsIn cells, MTII was only weakly able to raise calcium levels in GH3 cells.

    Peptide
    Melanotan II
    Outcome
    IncreaseIntracellular calcium increase in GH3 cells
    Study

    n: not reported in source

    GH3 cells
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    MTII, a potent synthetic agonist of the MC3, MC4, and MC5 receptor as well as an N-terminally extended recombinant analog of gamma3MSH showed low potency in increasing [Ca(2+)](i) in GH3 cells, but high potency in stimulating cAMP accumulation in HEK 293 cells stably transfected with the MC3 receptor.
    1 context sentence
    Summary · context
    In the present study we report that gamma3MSH and some analogs increase [Ca(2+)](i) in the GH- and PRL-secreting GH3 cell line and evaluate on the basis of pharmacological experiments and gene expression studies which MC receptor may be involved.
  2. 02LabIn vitro (cells)Activity measurement

    In HEK 293 cells stably transfected with the MC3 receptor, MTII showed high potency in stimulating cAMP accumulation.

    In plain wordsIn cells, MTII strongly stimulated cAMP production in cells carrying the MC3 receptor.

    Peptide
    Melanotan II
    Outcome
    IncreasecAMP accumulation in MC3 receptor-expressing HEK 293 cells
    Study

    n: not reported in source

    HEK 293 cells expressing the MC3 receptor
    Outcome group
    Receptor activity and mechanism · Endocrine and hormones
    Studied dose
    Dose not stated

    Source sentences

    Summary
    MTII, a potent synthetic agonist of the MC3, MC4, and MC5 receptor as well as an N-terminally extended recombinant analog of gamma3MSH showed low potency in increasing [Ca(2+)](i) in GH3 cells, but high potency in stimulating cAMP accumulation in HEK 293 cells stably transfected with the MC3 receptor.
    1 context sentence
    Summary · context
    In the present study we report that gamma3MSH and some analogs increase [Ca(2+)](i) in the GH- and PRL-secreting GH3 cell line and evaluate on the basis of pharmacological experiments and gene expression studies which MC receptor may be involved.

AI wrote and checked these results. AI can make mistakes, so check important results against the source.

Version history

PepBank keeps every version of the data on this study. Status changes from Retraction Watch, Europe PMC and Crossref are listed with their source.

  1. Version 2Current

    10 Oct 2026, 09:43 UTC

    Study results updated

  2. Version 1

    9 Oct 2026, 03:04 UTC

    First added · source update from Europe PMC

Subjects

Terms as the source lists them.

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