Des (1-3) IGF-I increased IGF-I mRNA levels 2.6-fold in cultured rat granulosa cells.
In plain wordsIn cells from rats, des (1-3) IGF-I increased the genetic message for IGF-I.
- Peptide
- IGF-1 DES (des(1-3)IGF-I)
- Outcome
- IncreaseIGF-I mRNA
- Study
ratn: not reported in source
estrogen-primed rat granulosa cells- Outcome group
- Receptor activity and mechanism · Reproductive and sexual health
- Studied dose
- Dose not stated · 72 hAs given in this study (rat).
Measurements
- IGF-I mRNA increase
- 2.6 fold
- des (1-3) IGF-I
Source sentences
Treatment with insulin and des (1-3) IGF-I proved equally effective, producing 2.0- and 2.6-fold increases, respectively, thereby suggesting that the IGF-I effect may be mediated via the type 1 IGF receptor.
3 context sentences(design, population, supporting numbers)
Treatment of cultured rat granulosa cells with IGF-I resulted in a significant 1.8-fold increase in the steady-state levels of IGF-I mRNA.
Granulosa cells, obtained by follicular puncture from 25-d-old estrogen-primed rats were cultured in polystyrene tubes for 72 h under serum-free conditions, in the absence or presence of the indicated agents.
At the conclusion of each experiment, media were discarded, and RNA was extracted and subjected to an RNase protection assay.